Sequential Application of Antioxidants Rectifies Ion Imbalance and Strengthens Antioxidant Systems in Salt-Stressed Cucumber
Exogenous antioxidant applications enable salt-stressed plants to successfully cope with different environmental stresses. The objectives of this investigation were to study the effects of sequential treatments of proline (Pro), ascorbic acid (AsA), and/or glutathione (GSH) on 100 mM NaCl-stressed c...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-12-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/9/12/1783 |
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| author | Mahmoud F. Seleiman Wael M. Semida Mostafa M. Rady Gamal F. Mohamed Khaulood A. Hemida Bushra Ahmed Alhammad Mohamed M. Hassan Ashwag Shami |
| author_facet | Mahmoud F. Seleiman Wael M. Semida Mostafa M. Rady Gamal F. Mohamed Khaulood A. Hemida Bushra Ahmed Alhammad Mohamed M. Hassan Ashwag Shami |
| author_sort | Mahmoud F. Seleiman |
| collection | DOAJ |
| description | Exogenous antioxidant applications enable salt-stressed plants to successfully cope with different environmental stresses. The objectives of this investigation were to study the effects of sequential treatments of proline (Pro), ascorbic acid (AsA), and/or glutathione (GSH) on 100 mM NaCl-stressed cucumber transplant’s physio-biochemical and growth traits as well as systems of antioxidant defense. Under salinity stress, different treatment of AsA, Pro, or/and GSH improved growth characteristics, stomatal conductance (gs), enhanced the activities of glutathione reductase (GR), superoxide dismutase (SOD), ascorbate peroxidase (APX), and catalase (CAT) as well as increased contents of AsA, Pro, and GSH. However, sequential application of antioxidants (GSH-Pro- AsA) significantly exceeded all individual applications, reducing leaf and root Cd<sup>2+</sup> and Na<sup>+</sup> contents in comparison to the control. In plants grown under NaCl-salt stress, growth characteristics, photosynthetic efficiency, membrane stability index (MSI), relative water content (RWC), contents of root and leaf K<sup>+</sup> and Ca<sup>2+</sup>, and ratios of K<sup>+</sup>/Na<sup>+</sup> and Ca<sup>2+</sup>/Na<sup>+</sup> were notably reduced, while leaf contents of non-enzymatic and enzymatic antioxidants, as well as root and leaf Cd<sup>2+</sup> and Na<sup>+</sup> concentrations were remarkably increased. However, AsA, Pro, or/and GSH treatments significantly improved all investigated growth characteristics, photosynthetic efficiency, RWC and MSI, as well as AsA, Pro, and GSH, and enzymatic activity, leaf and root K<sup>+</sup> and Ca<sup>2+</sup> contents and their ratios to Na<sup>+</sup>, while significantly reduced leaf and root Cd<sup>2+</sup> and Na<sup>+</sup> contents. |
| first_indexed | 2024-03-10T14:01:58Z |
| format | Article |
| id | doaj.art-539e1b8596b54a8fa911b7e840f99ccf |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T14:01:58Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-539e1b8596b54a8fa911b7e840f99ccf2023-11-21T01:01:36ZengMDPI AGPlants2223-77472020-12-01912178310.3390/plants9121783Sequential Application of Antioxidants Rectifies Ion Imbalance and Strengthens Antioxidant Systems in Salt-Stressed CucumberMahmoud F. Seleiman0Wael M. Semida1Mostafa M. Rady2Gamal F. Mohamed3Khaulood A. Hemida4Bushra Ahmed Alhammad5Mohamed M. Hassan6Ashwag Shami7Plant Production Department, College of Food and Agriculture Sciences, King Saud University, P.O. Box 2460, Riyadh 11451, Saudi ArabiaHorticulture Department, Faculty of Agriculture, Fayoum University, Fayoum 63514, EgyptBotany Department, Faculty of Agriculture, Fayoum University, Fayoum 63514, EgyptBotany Department, Faculty of Agriculture, Fayoum University, Fayoum 63514, EgyptBotany Department, Faculty of Science, Fayoum University, Fayoum 63514, EgyptBiology Department, College of Science and Humanity Studies, Prince Sattam Bin Abdulaziz University, Al Kharj Box 292, Riyadh 11942, Saudi ArabiaDepartment of Biology, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaBiology Department, College of Sciences, Princess Nourah Bint Abdulrahman University, Riyadh 11617, Saudi ArabiaExogenous antioxidant applications enable salt-stressed plants to successfully cope with different environmental stresses. The objectives of this investigation were to study the effects of sequential treatments of proline (Pro), ascorbic acid (AsA), and/or glutathione (GSH) on 100 mM NaCl-stressed cucumber transplant’s physio-biochemical and growth traits as well as systems of antioxidant defense. Under salinity stress, different treatment of AsA, Pro, or/and GSH improved growth characteristics, stomatal conductance (gs), enhanced the activities of glutathione reductase (GR), superoxide dismutase (SOD), ascorbate peroxidase (APX), and catalase (CAT) as well as increased contents of AsA, Pro, and GSH. However, sequential application of antioxidants (GSH-Pro- AsA) significantly exceeded all individual applications, reducing leaf and root Cd<sup>2+</sup> and Na<sup>+</sup> contents in comparison to the control. In plants grown under NaCl-salt stress, growth characteristics, photosynthetic efficiency, membrane stability index (MSI), relative water content (RWC), contents of root and leaf K<sup>+</sup> and Ca<sup>2+</sup>, and ratios of K<sup>+</sup>/Na<sup>+</sup> and Ca<sup>2+</sup>/Na<sup>+</sup> were notably reduced, while leaf contents of non-enzymatic and enzymatic antioxidants, as well as root and leaf Cd<sup>2+</sup> and Na<sup>+</sup> concentrations were remarkably increased. However, AsA, Pro, or/and GSH treatments significantly improved all investigated growth characteristics, photosynthetic efficiency, RWC and MSI, as well as AsA, Pro, and GSH, and enzymatic activity, leaf and root K<sup>+</sup> and Ca<sup>2+</sup> contents and their ratios to Na<sup>+</sup>, while significantly reduced leaf and root Cd<sup>2+</sup> and Na<sup>+</sup> contents.https://www.mdpi.com/2223-7747/9/12/1783sequential application of antioxidantssalinity<i>Cucumis sativus</i>photosynthetic efficiencyantioxidant defense systems |
| spellingShingle | Mahmoud F. Seleiman Wael M. Semida Mostafa M. Rady Gamal F. Mohamed Khaulood A. Hemida Bushra Ahmed Alhammad Mohamed M. Hassan Ashwag Shami Sequential Application of Antioxidants Rectifies Ion Imbalance and Strengthens Antioxidant Systems in Salt-Stressed Cucumber Plants sequential application of antioxidants salinity <i>Cucumis sativus</i> photosynthetic efficiency antioxidant defense systems |
| title | Sequential Application of Antioxidants Rectifies Ion Imbalance and Strengthens Antioxidant Systems in Salt-Stressed Cucumber |
| title_full | Sequential Application of Antioxidants Rectifies Ion Imbalance and Strengthens Antioxidant Systems in Salt-Stressed Cucumber |
| title_fullStr | Sequential Application of Antioxidants Rectifies Ion Imbalance and Strengthens Antioxidant Systems in Salt-Stressed Cucumber |
| title_full_unstemmed | Sequential Application of Antioxidants Rectifies Ion Imbalance and Strengthens Antioxidant Systems in Salt-Stressed Cucumber |
| title_short | Sequential Application of Antioxidants Rectifies Ion Imbalance and Strengthens Antioxidant Systems in Salt-Stressed Cucumber |
| title_sort | sequential application of antioxidants rectifies ion imbalance and strengthens antioxidant systems in salt stressed cucumber |
| topic | sequential application of antioxidants salinity <i>Cucumis sativus</i> photosynthetic efficiency antioxidant defense systems |
| url | https://www.mdpi.com/2223-7747/9/12/1783 |
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