Rifampicin-loaded electrospun polycaprolactone membranes: Characterization of stability, antibacterial effects and urotheliocytes proliferation
Pathological conditions such as infections, cancer, inflammation, or iatrogenic lesions, can hinder the correct functionality of the ureter and its structural integrity. Several strategies for the regeneration of the ureter and the restoration of its functionality are available but the best strategy...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | Materials & Design |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S026412752200908X |
| _version_ | 1811199376439115776 |
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| author | Luigi Musciacchio Mario Mardirossian Benedetta Guagnini Alessandra Raffini Michele Rizzo Carlo Trombetta Giovanni Liguori Gianluca Turco Davide Porrelli |
| author_facet | Luigi Musciacchio Mario Mardirossian Benedetta Guagnini Alessandra Raffini Michele Rizzo Carlo Trombetta Giovanni Liguori Gianluca Turco Davide Porrelli |
| author_sort | Luigi Musciacchio |
| collection | DOAJ |
| description | Pathological conditions such as infections, cancer, inflammation, or iatrogenic lesions, can hinder the correct functionality of the ureter and its structural integrity. Several strategies for the regeneration of the ureter and the restoration of its functionality are available but the best strategy has not been reached yet. Among the proposed strategies, a promising one is the development of tubular scaffolds, in particular exploiting electrospinning technique. In this work, antibacterial electrospun polycaprolactone/rifampicin (PCL/Rif) membranes were prepared and characterized. The membranes are characterized by randomly oriented nanofibers with a homogeneous diameter, as determined by scanning electron microscopy. The mechanical characterization, performed with uniaxial tensile tests, showed a suitable stability over time and a proper deformability. The rifampicin release, investigated by UV spectrophotometry, showed a burst release in the first part of the experiment and a sustained release over time. The membranes are biocompatible and able to support the adhesion and proliferation of urotheliocytes. Moreover, PCL/Rif membranes showed an antibacterial activity against Escherichia coli and other bacterial strains belonging to the “ESKAPE” group. Considering the possibility to use the electrospinning for the production of tubular scaffolds, the described membranes represent a promising starting point for the preparation of ureteral scaffolds with antibacterial properties. |
| first_indexed | 2024-04-12T01:47:17Z |
| format | Article |
| id | doaj.art-53a22c58faab45f595d4179a0f8b138a |
| institution | Directory Open Access Journal |
| issn | 0264-1275 |
| language | English |
| last_indexed | 2024-04-12T01:47:17Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials & Design |
| spelling | doaj.art-53a22c58faab45f595d4179a0f8b138a2022-12-22T03:53:02ZengElsevierMaterials & Design0264-12752022-12-01224111286Rifampicin-loaded electrospun polycaprolactone membranes: Characterization of stability, antibacterial effects and urotheliocytes proliferationLuigi Musciacchio0Mario Mardirossian1Benedetta Guagnini2Alessandra Raffini3Michele Rizzo4Carlo Trombetta5Giovanni Liguori6Gianluca Turco7Davide Porrelli8Department of Medicine, Surgery and Health Sciences, University of Trieste, Piazza dell’Ospitale 1, 34125 Trieste, ItalyDepartment of Medicine, Surgery and Health Sciences, University of Trieste, Piazza dell’Ospitale 1, 34125 Trieste, Italy; Corresponding authors at: Department of Life Sciences, University of Trieste, Via Licio Giorgieri 5, 34127 Trieste, Italy (M. Mardirossian), Department of Life Sciences, University of Trieste, Via Alexander Fleming 31/B, 34127 Trieste, Italy (D. Porrelli).Department of Medicine, Surgery and Health Sciences, University of Trieste, Piazza dell’Ospitale 1, 34125 Trieste, ItalyDepartment of Engineering and Architecture, University of Trieste, Via Alfonso Valerio, 6/1 – 34127 Trieste (TS), ItalyDepartment of Medicine, Surgery and Health Sciences, University of Trieste, Strada di Fiume 447, 34149 Trieste, ItalyDepartment of Medicine, Surgery and Health Sciences, University of Trieste, Strada di Fiume 447, 34149 Trieste, ItalyDepartment of Medicine, Surgery and Health Sciences, University of Trieste, Strada di Fiume 447, 34149 Trieste, ItalyDepartment of Medicine, Surgery and Health Sciences, University of Trieste, Piazza dell’Ospitale 1, 34125 Trieste, ItalyDepartment of Medicine, Surgery and Health Sciences, University of Trieste, Piazza dell’Ospitale 1, 34125 Trieste, Italy; Corresponding authors at: Department of Life Sciences, University of Trieste, Via Licio Giorgieri 5, 34127 Trieste, Italy (M. Mardirossian), Department of Life Sciences, University of Trieste, Via Alexander Fleming 31/B, 34127 Trieste, Italy (D. Porrelli).Pathological conditions such as infections, cancer, inflammation, or iatrogenic lesions, can hinder the correct functionality of the ureter and its structural integrity. Several strategies for the regeneration of the ureter and the restoration of its functionality are available but the best strategy has not been reached yet. Among the proposed strategies, a promising one is the development of tubular scaffolds, in particular exploiting electrospinning technique. In this work, antibacterial electrospun polycaprolactone/rifampicin (PCL/Rif) membranes were prepared and characterized. The membranes are characterized by randomly oriented nanofibers with a homogeneous diameter, as determined by scanning electron microscopy. The mechanical characterization, performed with uniaxial tensile tests, showed a suitable stability over time and a proper deformability. The rifampicin release, investigated by UV spectrophotometry, showed a burst release in the first part of the experiment and a sustained release over time. The membranes are biocompatible and able to support the adhesion and proliferation of urotheliocytes. Moreover, PCL/Rif membranes showed an antibacterial activity against Escherichia coli and other bacterial strains belonging to the “ESKAPE” group. Considering the possibility to use the electrospinning for the production of tubular scaffolds, the described membranes represent a promising starting point for the preparation of ureteral scaffolds with antibacterial properties.http://www.sciencedirect.com/science/article/pii/S026412752200908XAntibacterialBiomaterialElectrospinningPolycaprolactoneRifampicinUreteral regeneration |
| spellingShingle | Luigi Musciacchio Mario Mardirossian Benedetta Guagnini Alessandra Raffini Michele Rizzo Carlo Trombetta Giovanni Liguori Gianluca Turco Davide Porrelli Rifampicin-loaded electrospun polycaprolactone membranes: Characterization of stability, antibacterial effects and urotheliocytes proliferation Materials & Design Antibacterial Biomaterial Electrospinning Polycaprolactone Rifampicin Ureteral regeneration |
| title | Rifampicin-loaded electrospun polycaprolactone membranes: Characterization of stability, antibacterial effects and urotheliocytes proliferation |
| title_full | Rifampicin-loaded electrospun polycaprolactone membranes: Characterization of stability, antibacterial effects and urotheliocytes proliferation |
| title_fullStr | Rifampicin-loaded electrospun polycaprolactone membranes: Characterization of stability, antibacterial effects and urotheliocytes proliferation |
| title_full_unstemmed | Rifampicin-loaded electrospun polycaprolactone membranes: Characterization of stability, antibacterial effects and urotheliocytes proliferation |
| title_short | Rifampicin-loaded electrospun polycaprolactone membranes: Characterization of stability, antibacterial effects and urotheliocytes proliferation |
| title_sort | rifampicin loaded electrospun polycaprolactone membranes characterization of stability antibacterial effects and urotheliocytes proliferation |
| topic | Antibacterial Biomaterial Electrospinning Polycaprolactone Rifampicin Ureteral regeneration |
| url | http://www.sciencedirect.com/science/article/pii/S026412752200908X |
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