Establishing and characterising a new cell line from Calliphora vicina (diptera: calliphoridae) fly embryonic tissues
Insect cell lines represent a promising and expanding field as they have several research applications including biotechnology, virology, immunity, toxicology, cell signalling mechanisms and evolution. They constitute a powerful tool having a direct impact on human and veterinary medicine and agricu...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-09-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844022019624 |
| _version_ | 1828384599891247104 |
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| author | Ingred Pinillos Cindy Pérez Orlando Torres Manuel A. Patarroyo Felio J. Bello |
| author_facet | Ingred Pinillos Cindy Pérez Orlando Torres Manuel A. Patarroyo Felio J. Bello |
| author_sort | Ingred Pinillos |
| collection | DOAJ |
| description | Insect cell lines represent a promising and expanding field as they have several research applications including biotechnology, virology, immunity, toxicology, cell signalling mechanisms and evolution. They constitute a powerful tool having a direct impact on human and veterinary medicine and agriculture. Although more than 1000 cell lines have currently been established from various insect species, Calliphora vicina-derived fly cell lines are lacking. This study was aimed at establishing a new C. vicina embryonic tissue-derived cell line. Adult flies were collected and embryonated eggs were mechanically homogenised and seeded in four types of culture media (L15, Grace's insect medium, Grace's/L15 and DMEM). Cell growth and morphological characteristics were recorded and cytogenetic and molecular patterns were determined. The CV-062020-PPB cell line was established and was shown to have optimal growth in Grace's/L15 medium. CV-062020-PPB cell monolayers that had been sub-cultured over 16 times consisted of firmly adhering cells having different morphologies; a fibroblast-like shape dominated and the karyotype had a 12-chromosome diploid number. RAPD-PCR analysis of the CV-062020-PPB cell line revealed a high similarity index and strong intraspecific relationship with C. vicina adult flies and a weaker relationship with the Lutzomyia longipalpis-derived cell line (Lulo). The CV-062020-PPB cell line constitutes the first cell line obtained from C. vicina embryonic tissues and represents an important basic and applied research tool. |
| first_indexed | 2024-12-10T05:06:06Z |
| format | Article |
| id | doaj.art-53a9ce8b564c4c6582f998539d88183c |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-12-10T05:06:06Z |
| publishDate | 2022-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-53a9ce8b564c4c6582f998539d88183c2022-12-22T02:01:14ZengElsevierHeliyon2405-84402022-09-0189e10674Establishing and characterising a new cell line from Calliphora vicina (diptera: calliphoridae) fly embryonic tissuesIngred Pinillos0Cindy Pérez1Orlando Torres2Manuel A. Patarroyo3Felio J. Bello4Universidad de La Salle, Facultad de Ciencias Agropecuarias, Programa de Medicina Veterinaria, Bogotá DC, Colombia; Universidad Colegio Mayor de Cundinamarca, Facultad de Ciencias de La Salud, Bogotá DC, ColombiaUniversidad Antonio Nariño, Facultad de Medicina Veterinaria, Bogotá DC, ColombiaUniversidad Antonio Nariño, Facultad de Medicina Veterinaria, Bogotá DC, ColombiaFundación Instituto de Inmunología de Colombia (FIDIC), Molecular Biology and Immunology Department, Bogotá DC, Colombia; Universidad Santo Tomás, Health Sciences Division, Main Campus, Bogotá DC, Colombia; Universidad Nacional de Colombia, Faculty of Medicine, Microbiology Department, Bogotá DC, ColombiaUniversidad de La Salle, Facultad de Ciencias Agropecuarias, Programa de Medicina Veterinaria, Bogotá DC, Colombia; Corresponding author.Insect cell lines represent a promising and expanding field as they have several research applications including biotechnology, virology, immunity, toxicology, cell signalling mechanisms and evolution. They constitute a powerful tool having a direct impact on human and veterinary medicine and agriculture. Although more than 1000 cell lines have currently been established from various insect species, Calliphora vicina-derived fly cell lines are lacking. This study was aimed at establishing a new C. vicina embryonic tissue-derived cell line. Adult flies were collected and embryonated eggs were mechanically homogenised and seeded in four types of culture media (L15, Grace's insect medium, Grace's/L15 and DMEM). Cell growth and morphological characteristics were recorded and cytogenetic and molecular patterns were determined. The CV-062020-PPB cell line was established and was shown to have optimal growth in Grace's/L15 medium. CV-062020-PPB cell monolayers that had been sub-cultured over 16 times consisted of firmly adhering cells having different morphologies; a fibroblast-like shape dominated and the karyotype had a 12-chromosome diploid number. RAPD-PCR analysis of the CV-062020-PPB cell line revealed a high similarity index and strong intraspecific relationship with C. vicina adult flies and a weaker relationship with the Lutzomyia longipalpis-derived cell line (Lulo). The CV-062020-PPB cell line constitutes the first cell line obtained from C. vicina embryonic tissues and represents an important basic and applied research tool.http://www.sciencedirect.com/science/article/pii/S2405844022019624Calliphora vicinaCV-062020-PPBCell morphologyKaryotypeRAPD-PCR |
| spellingShingle | Ingred Pinillos Cindy Pérez Orlando Torres Manuel A. Patarroyo Felio J. Bello Establishing and characterising a new cell line from Calliphora vicina (diptera: calliphoridae) fly embryonic tissues Heliyon Calliphora vicina CV-062020-PPB Cell morphology Karyotype RAPD-PCR |
| title | Establishing and characterising a new cell line from Calliphora vicina (diptera: calliphoridae) fly embryonic tissues |
| title_full | Establishing and characterising a new cell line from Calliphora vicina (diptera: calliphoridae) fly embryonic tissues |
| title_fullStr | Establishing and characterising a new cell line from Calliphora vicina (diptera: calliphoridae) fly embryonic tissues |
| title_full_unstemmed | Establishing and characterising a new cell line from Calliphora vicina (diptera: calliphoridae) fly embryonic tissues |
| title_short | Establishing and characterising a new cell line from Calliphora vicina (diptera: calliphoridae) fly embryonic tissues |
| title_sort | establishing and characterising a new cell line from calliphora vicina diptera calliphoridae fly embryonic tissues |
| topic | Calliphora vicina CV-062020-PPB Cell morphology Karyotype RAPD-PCR |
| url | http://www.sciencedirect.com/science/article/pii/S2405844022019624 |
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