3-aminophenylboronic acid conjugation on responsive polymer and gold nanoparticles for qualitative bacterial detection
Background: Because of their sensitive and selective responses to a wide variety of analytes, colorimetric sensors have gained widespread acceptance in recent years. Gold nanoparticles (AuNPs) are widely employed in visual sensor strategies due to their high stability and ease of use. Combining AuN...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer Medknow Publications
2023-01-01
|
| Series: | Journal of Pharmacy and Bioallied Sciences |
| Subjects: | |
| Online Access: | http://www.jpbsonline.org/article.asp?issn=0975-7406;year=2023;volume=15;issue=2;spage=81;epage=87;aulast=Wikantyasning |
| _version_ | 1797773810989006848 |
|---|---|
| author | Erindyah Retno Wikantyasning Muhammad Da'i Zakky Cholisoh Ummi Kalsum |
| author_facet | Erindyah Retno Wikantyasning Muhammad Da'i Zakky Cholisoh Ummi Kalsum |
| author_sort | Erindyah Retno Wikantyasning |
| collection | DOAJ |
| description | Background: Because of their sensitive and selective responses to a wide variety of analytes, colorimetric sensors have gained widespread acceptance in recent years. Gold nanoparticles (AuNPs) are widely employed in visual sensor strategies due to their high stability and ease of use. Combining AuNPs with a responsive polymer can result in distinct surface plasmon resonance (SPR) changes that can be utilized as colorimetric biosensors. Objectives: The purpose of this research is to develop a colorimetric-based sensor through the utilization of the optical properties of gold nanoparticles (AuNPs) crosslinked with pH-responsive polymers poly (acrylic acid) (PAA) conjugated to 3-aminophenyl boronic acid (APBA). Methods: The polymer (PAA) was synthesized via RAFT polymerization. The inversed Turkevic method was used to produce AuNPs, which were subsequently used in a self-assembly process using poly (acrylic acid)-aminophenyl boronic acid (PAA-APBA) to create the self-assembled AuNPs-APBA-PAA. The particle size, zeta potential, and reversibility of the polymer-modified gold nanoparticles were determined using a transmission electron microscope (TEM), a particle size analyzer (PSA), and an Ultraviolet-Visible spectrophotometer (UV-Vis spectrophotometer). Visual, UV-Vis spectrophotometer and TEM observations confirmed the system's ability to identify bacteria. Statistical analysis was performed using a one-way analysis of variance using Excel software. Results: Using UV-Vis spectrophotometry, the particle size of AuNPs was determined to be 25.7 nm, and the maximum absorbance occurred at 530 nm. AuNPs PAA APBA colloid exhibited an absorbance maximum of 532 nm, a zeta potential of -41.53, and a pH transition point between 4 and 5. At E. coli concentrations of 4.5 x 107 CFU/mL, the color of the system sensors changed from red to blue after 15 hours of incubation, whereas at S. aureus concentrations of 1.2 x 109 CFU/mL, the color changed to purple immediately after mixing. The TEM confirmed that the detection mechanism is based on the boronate-polyol bonding of saccharides on the outer membranes of Escherichia coli and Staphylococcus aureus. Conclusions: The use of APBA in conjunction with pH-responsive PAA polymers containing AuNPs to detect E. coli and S. aureus bacteria induces a maximum wavelength transition, followed by a color change from red to blue. By the process of de-swelling of the responsive polymer, which induces the aggregation of the AuNPs, the established sensor system is able to alter the color. The conjugated polymer and gold nanoparticle-based sensor system demonstrated a promising method for bacterial detection. |
| first_indexed | 2024-03-12T22:10:53Z |
| format | Article |
| id | doaj.art-53ab216505254967b777b896f2baa0ac |
| institution | Directory Open Access Journal |
| issn | 0975-7406 |
| language | English |
| last_indexed | 2024-03-12T22:10:53Z |
| publishDate | 2023-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Pharmacy and Bioallied Sciences |
| spelling | doaj.art-53ab216505254967b777b896f2baa0ac2023-07-23T11:42:04ZengWolters Kluwer Medknow PublicationsJournal of Pharmacy and Bioallied Sciences0975-74062023-01-01152818710.4103/jpbs.jpbs_646_223-aminophenylboronic acid conjugation on responsive polymer and gold nanoparticles for qualitative bacterial detectionErindyah Retno WikantyasningMuhammad Da'iZakky CholisohUmmi KalsumBackground: Because of their sensitive and selective responses to a wide variety of analytes, colorimetric sensors have gained widespread acceptance in recent years. Gold nanoparticles (AuNPs) are widely employed in visual sensor strategies due to their high stability and ease of use. Combining AuNPs with a responsive polymer can result in distinct surface plasmon resonance (SPR) changes that can be utilized as colorimetric biosensors. Objectives: The purpose of this research is to develop a colorimetric-based sensor through the utilization of the optical properties of gold nanoparticles (AuNPs) crosslinked with pH-responsive polymers poly (acrylic acid) (PAA) conjugated to 3-aminophenyl boronic acid (APBA). Methods: The polymer (PAA) was synthesized via RAFT polymerization. The inversed Turkevic method was used to produce AuNPs, which were subsequently used in a self-assembly process using poly (acrylic acid)-aminophenyl boronic acid (PAA-APBA) to create the self-assembled AuNPs-APBA-PAA. The particle size, zeta potential, and reversibility of the polymer-modified gold nanoparticles were determined using a transmission electron microscope (TEM), a particle size analyzer (PSA), and an Ultraviolet-Visible spectrophotometer (UV-Vis spectrophotometer). Visual, UV-Vis spectrophotometer and TEM observations confirmed the system's ability to identify bacteria. Statistical analysis was performed using a one-way analysis of variance using Excel software. Results: Using UV-Vis spectrophotometry, the particle size of AuNPs was determined to be 25.7 nm, and the maximum absorbance occurred at 530 nm. AuNPs PAA APBA colloid exhibited an absorbance maximum of 532 nm, a zeta potential of -41.53, and a pH transition point between 4 and 5. At E. coli concentrations of 4.5 x 107 CFU/mL, the color of the system sensors changed from red to blue after 15 hours of incubation, whereas at S. aureus concentrations of 1.2 x 109 CFU/mL, the color changed to purple immediately after mixing. The TEM confirmed that the detection mechanism is based on the boronate-polyol bonding of saccharides on the outer membranes of Escherichia coli and Staphylococcus aureus. Conclusions: The use of APBA in conjunction with pH-responsive PAA polymers containing AuNPs to detect E. coli and S. aureus bacteria induces a maximum wavelength transition, followed by a color change from red to blue. By the process of de-swelling of the responsive polymer, which induces the aggregation of the AuNPs, the established sensor system is able to alter the color. The conjugated polymer and gold nanoparticle-based sensor system demonstrated a promising method for bacterial detection.http://www.jpbsonline.org/article.asp?issn=0975-7406;year=2023;volume=15;issue=2;spage=81;epage=87;aulast=Wikantyasningbacteriaboronic acidcolorimetric sensorsgold nanoparticlessensitive polymer |
| spellingShingle | Erindyah Retno Wikantyasning Muhammad Da'i Zakky Cholisoh Ummi Kalsum 3-aminophenylboronic acid conjugation on responsive polymer and gold nanoparticles for qualitative bacterial detection Journal of Pharmacy and Bioallied Sciences bacteria boronic acid colorimetric sensors gold nanoparticles sensitive polymer |
| title | 3-aminophenylboronic acid conjugation on responsive polymer and gold nanoparticles for qualitative bacterial detection |
| title_full | 3-aminophenylboronic acid conjugation on responsive polymer and gold nanoparticles for qualitative bacterial detection |
| title_fullStr | 3-aminophenylboronic acid conjugation on responsive polymer and gold nanoparticles for qualitative bacterial detection |
| title_full_unstemmed | 3-aminophenylboronic acid conjugation on responsive polymer and gold nanoparticles for qualitative bacterial detection |
| title_short | 3-aminophenylboronic acid conjugation on responsive polymer and gold nanoparticles for qualitative bacterial detection |
| title_sort | 3 aminophenylboronic acid conjugation on responsive polymer and gold nanoparticles for qualitative bacterial detection |
| topic | bacteria boronic acid colorimetric sensors gold nanoparticles sensitive polymer |
| url | http://www.jpbsonline.org/article.asp?issn=0975-7406;year=2023;volume=15;issue=2;spage=81;epage=87;aulast=Wikantyasning |
| work_keys_str_mv | AT erindyahretnowikantyasning 3aminophenylboronicacidconjugationonresponsivepolymerandgoldnanoparticlesforqualitativebacterialdetection AT muhammaddai 3aminophenylboronicacidconjugationonresponsivepolymerandgoldnanoparticlesforqualitativebacterialdetection AT zakkycholisoh 3aminophenylboronicacidconjugationonresponsivepolymerandgoldnanoparticlesforqualitativebacterialdetection AT ummikalsum 3aminophenylboronicacidconjugationonresponsivepolymerandgoldnanoparticlesforqualitativebacterialdetection |