Pomegranate (<i>Punica granatum</i> L.) Peel Extracts as Antimicrobial and Antioxidant Additives Used in Alfalfa Sprouts
Aqueous and ethanolic pomegranate peel extracts (PPE) were studied as a source of phenolic compounds with antimicrobial, anti-quorum sensing, and antioxidant properties. The aqueous extract showed higher total phenolic and flavonoid content (153.43 mg GAE/g and 45.74, respectively) and antioxidant c...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-08-01
|
| Series: | Foods |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2304-8158/11/17/2588 |
| _version_ | 1827666310326124544 |
|---|---|
| author | Manuel Reynaldo Cruz-Valenzuela Rosa E. Ayala-Soto Jesus Fernando Ayala-Zavala Brenda A. Espinoza-Silva Gustavo A. González-Aguilar Olga Martín-Belloso Robert Soliva-Fortuny Filomena Nazzaro Florinda Fratianni Melvin R. Tapia-Rodríguez Ariadna Thalia Bernal-Mercado |
| author_facet | Manuel Reynaldo Cruz-Valenzuela Rosa E. Ayala-Soto Jesus Fernando Ayala-Zavala Brenda A. Espinoza-Silva Gustavo A. González-Aguilar Olga Martín-Belloso Robert Soliva-Fortuny Filomena Nazzaro Florinda Fratianni Melvin R. Tapia-Rodríguez Ariadna Thalia Bernal-Mercado |
| author_sort | Manuel Reynaldo Cruz-Valenzuela |
| collection | DOAJ |
| description | Aqueous and ethanolic pomegranate peel extracts (PPE) were studied as a source of phenolic compounds with antimicrobial, anti-quorum sensing, and antioxidant properties. The aqueous extract showed higher total phenolic and flavonoid content (153.43 mg GAE/g and 45.74, respectively) and antioxidant capacity (DPPH radical inhibition: 86.12%, ABTS radical scavenging capacity: 958.21 mg TE/dw) compared to the ethanolic extract. The main phenolic compounds identified by UPLC-DAD were chlorogenic and gallic acids. The aqueous PPE extract showed antimicrobial activity against <i>Listeria monocytogenes</i>, <i>Salmonella</i> Typhimurium, <i>Candida tropicalis</i> (MICs 19–30 mg/mL), and anti-quorum sensing activity expressed as inhibition of <i>Chromobacterium violaceum</i> violacein production (%). The aqueous PPE extracts at 25 mg/mL applied on alfalfa sprouts reduced psychrophilic bacteria (1.12 Log CFU/100 g) and total coliforms (1.23 Log CFU/100 g) and increased the antioxidant capacity of the treated sprouts (55.13 µmol TE/100 g (DPPH) and 126.56 µmol TE/100 g (ABTS)) compared to untreated alfalfa. This study emphasizes PPE’s antioxidant and antimicrobial activities in alfalfa sprouts preservation. |
| first_indexed | 2024-03-10T01:49:58Z |
| format | Article |
| id | doaj.art-53ac776fc45e435e81a23aba604a7b8d |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-10T01:49:58Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-53ac776fc45e435e81a23aba604a7b8d2023-11-23T13:07:53ZengMDPI AGFoods2304-81582022-08-011117258810.3390/foods11172588Pomegranate (<i>Punica granatum</i> L.) Peel Extracts as Antimicrobial and Antioxidant Additives Used in Alfalfa SproutsManuel Reynaldo Cruz-Valenzuela0Rosa E. Ayala-Soto1Jesus Fernando Ayala-Zavala2Brenda A. Espinoza-Silva3Gustavo A. González-Aguilar4Olga Martín-Belloso5Robert Soliva-Fortuny6Filomena Nazzaro7Florinda Fratianni8Melvin R. Tapia-Rodríguez9Ariadna Thalia Bernal-Mercado10Centro de Investigación en Alimentación y Desarrollo, A.C, Carretera Gustavo Enrique Astiazarán Rosas, No. 46, Col. La Victoria, Hermosillo 83304, MexicoCentro de Investigación en Alimentación y Desarrollo, A.C, Carretera Gustavo Enrique Astiazarán Rosas, No. 46, Col. La Victoria, Hermosillo 83304, MexicoCentro de Investigación en Alimentación y Desarrollo, A.C, Carretera Gustavo Enrique Astiazarán Rosas, No. 46, Col. La Victoria, Hermosillo 83304, MexicoCentro de Investigación en Alimentación y Desarrollo, A.C, Carretera Gustavo Enrique Astiazarán Rosas, No. 46, Col. La Victoria, Hermosillo 83304, MexicoCentro de Investigación en Alimentación y Desarrollo, A.C, Carretera Gustavo Enrique Astiazarán Rosas, No. 46, Col. La Victoria, Hermosillo 83304, MexicoDepartment of Food Technology, Agrotecnio Center, University of Lleida, Av. Rovira Roure 191, 25198 Lleida, SpainDepartment of Food Technology, Agrotecnio Center, University of Lleida, Av. Rovira Roure 191, 25198 Lleida, SpainIstituto di Scienze dell’Alimentazione, ISA-CN R, Via Roma, 64, 83100 Avellino, ItalyIstituto di Scienze dell’Alimentazione, ISA-CN R, Via Roma, 64, 83100 Avellino, ItalyDepartamento de Biotecnologia y Ciencias Alimentarias, Instituto Tecnológico de Sonora, 5 de Febrero 818 sur, Col. Centro, Ciudad Obregón 85000, MexicoDepartamento de Investigación y Posgrado en Alimentos, Universidad de Sonora, Blvd. Luis Encinas y Rosales S/N, Col. Centro, Hermosillo 83000, MexicoAqueous and ethanolic pomegranate peel extracts (PPE) were studied as a source of phenolic compounds with antimicrobial, anti-quorum sensing, and antioxidant properties. The aqueous extract showed higher total phenolic and flavonoid content (153.43 mg GAE/g and 45.74, respectively) and antioxidant capacity (DPPH radical inhibition: 86.12%, ABTS radical scavenging capacity: 958.21 mg TE/dw) compared to the ethanolic extract. The main phenolic compounds identified by UPLC-DAD were chlorogenic and gallic acids. The aqueous PPE extract showed antimicrobial activity against <i>Listeria monocytogenes</i>, <i>Salmonella</i> Typhimurium, <i>Candida tropicalis</i> (MICs 19–30 mg/mL), and anti-quorum sensing activity expressed as inhibition of <i>Chromobacterium violaceum</i> violacein production (%). The aqueous PPE extracts at 25 mg/mL applied on alfalfa sprouts reduced psychrophilic bacteria (1.12 Log CFU/100 g) and total coliforms (1.23 Log CFU/100 g) and increased the antioxidant capacity of the treated sprouts (55.13 µmol TE/100 g (DPPH) and 126.56 µmol TE/100 g (ABTS)) compared to untreated alfalfa. This study emphasizes PPE’s antioxidant and antimicrobial activities in alfalfa sprouts preservation.https://www.mdpi.com/2304-8158/11/17/2588food byproductphenolic compoundsanti-quorum sensing agentsnatural food additiveadded value |
| spellingShingle | Manuel Reynaldo Cruz-Valenzuela Rosa E. Ayala-Soto Jesus Fernando Ayala-Zavala Brenda A. Espinoza-Silva Gustavo A. González-Aguilar Olga Martín-Belloso Robert Soliva-Fortuny Filomena Nazzaro Florinda Fratianni Melvin R. Tapia-Rodríguez Ariadna Thalia Bernal-Mercado Pomegranate (<i>Punica granatum</i> L.) Peel Extracts as Antimicrobial and Antioxidant Additives Used in Alfalfa Sprouts Foods food byproduct phenolic compounds anti-quorum sensing agents natural food additive added value |
| title | Pomegranate (<i>Punica granatum</i> L.) Peel Extracts as Antimicrobial and Antioxidant Additives Used in Alfalfa Sprouts |
| title_full | Pomegranate (<i>Punica granatum</i> L.) Peel Extracts as Antimicrobial and Antioxidant Additives Used in Alfalfa Sprouts |
| title_fullStr | Pomegranate (<i>Punica granatum</i> L.) Peel Extracts as Antimicrobial and Antioxidant Additives Used in Alfalfa Sprouts |
| title_full_unstemmed | Pomegranate (<i>Punica granatum</i> L.) Peel Extracts as Antimicrobial and Antioxidant Additives Used in Alfalfa Sprouts |
| title_short | Pomegranate (<i>Punica granatum</i> L.) Peel Extracts as Antimicrobial and Antioxidant Additives Used in Alfalfa Sprouts |
| title_sort | pomegranate i punica granatum i l peel extracts as antimicrobial and antioxidant additives used in alfalfa sprouts |
| topic | food byproduct phenolic compounds anti-quorum sensing agents natural food additive added value |
| url | https://www.mdpi.com/2304-8158/11/17/2588 |
| work_keys_str_mv | AT manuelreynaldocruzvalenzuela pomegranateipunicagranatumilpeelextractsasantimicrobialandantioxidantadditivesusedinalfalfasprouts AT rosaeayalasoto pomegranateipunicagranatumilpeelextractsasantimicrobialandantioxidantadditivesusedinalfalfasprouts AT jesusfernandoayalazavala pomegranateipunicagranatumilpeelextractsasantimicrobialandantioxidantadditivesusedinalfalfasprouts AT brendaaespinozasilva pomegranateipunicagranatumilpeelextractsasantimicrobialandantioxidantadditivesusedinalfalfasprouts AT gustavoagonzalezaguilar pomegranateipunicagranatumilpeelextractsasantimicrobialandantioxidantadditivesusedinalfalfasprouts AT olgamartinbelloso pomegranateipunicagranatumilpeelextractsasantimicrobialandantioxidantadditivesusedinalfalfasprouts AT robertsolivafortuny pomegranateipunicagranatumilpeelextractsasantimicrobialandantioxidantadditivesusedinalfalfasprouts AT filomenanazzaro pomegranateipunicagranatumilpeelextractsasantimicrobialandantioxidantadditivesusedinalfalfasprouts AT florindafratianni pomegranateipunicagranatumilpeelextractsasantimicrobialandantioxidantadditivesusedinalfalfasprouts AT melvinrtapiarodriguez pomegranateipunicagranatumilpeelextractsasantimicrobialandantioxidantadditivesusedinalfalfasprouts AT ariadnathaliabernalmercado pomegranateipunicagranatumilpeelextractsasantimicrobialandantioxidantadditivesusedinalfalfasprouts |