Identification of genetic determinants of bedaquiline resistance in Mycobacterium tuberculosis in Ural region, Russia
ABSTRACTCollecting data on rare Mycobacterium tuberculosis (Mtb) clinical isolates with resistance to the new anti-tuberculosis drug bedaquiline is an important task for improving antimicrobial susceptibility testing methods. Nanopore whole genome sequencing, the proportion method on Middlebrook 7H1...
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| Format: | Article |
| Language: | English |
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American Society for Microbiology
2024-03-01
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| Series: | Microbiology Spectrum |
| Subjects: | |
| Online Access: | https://journals.asm.org/doi/10.1128/spectrum.03749-23 |
| _version_ | 1797276737869971456 |
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| author | Tatiana Umpeleva Elena Chetverikova Danila Belyaev Natalya Eremeeva Tatiana Boteva Ludmila Golubeva Diana Vakhrusheva Irina Vasilieva |
| author_facet | Tatiana Umpeleva Elena Chetverikova Danila Belyaev Natalya Eremeeva Tatiana Boteva Ludmila Golubeva Diana Vakhrusheva Irina Vasilieva |
| author_sort | Tatiana Umpeleva |
| collection | DOAJ |
| description | ABSTRACTCollecting data on rare Mycobacterium tuberculosis (Mtb) clinical isolates with resistance to the new anti-tuberculosis drug bedaquiline is an important task for improving antimicrobial susceptibility testing methods. Nanopore whole genome sequencing, the proportion method on Middlebrook 7H11 medium, and BACTEC MGIT 960 assays were used to analyze genotypic and phenotypic resistance to bedaquiline. We found four mutations: atpE I66M, atpE А63Р, Rv0678 А36Т, and Rv0678 S53P in five isolates with different levels of phenotypic bedaquiline resistance.IMPORTANCEBedaquiline (BDQ) is a new anti-tuberculosis drug. The phenotypic and genotypic data describing the mechanism of drug resistance are critical for the design of rapid and accurate diagnostic tests. We consider that our work, which describes genotypic and phenotypic resistance to BDQ, can contribute to the standardization of drug susceptibility testing. |
| first_indexed | 2024-03-07T15:33:34Z |
| format | Article |
| id | doaj.art-53ad8d02f02d40f5ad83321e65dcad08 |
| institution | Directory Open Access Journal |
| issn | 2165-0497 |
| language | English |
| last_indexed | 2024-03-07T15:33:34Z |
| publishDate | 2024-03-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | Microbiology Spectrum |
| spelling | doaj.art-53ad8d02f02d40f5ad83321e65dcad082024-03-05T14:04:36ZengAmerican Society for MicrobiologyMicrobiology Spectrum2165-04972024-03-0112310.1128/spectrum.03749-23Identification of genetic determinants of bedaquiline resistance in Mycobacterium tuberculosis in Ural region, RussiaTatiana Umpeleva0Elena Chetverikova1Danila Belyaev2Natalya Eremeeva3Tatiana Boteva4Ludmila Golubeva5Diana Vakhrusheva6Irina Vasilieva7Department of Microbiology and Preclinical Research, National Medical Research Center of Phthisiopulmonology and Infection Disease, Yekaterinburg, RussiaDepartment of Microbiology and Preclinical Research, National Medical Research Center of Phthisiopulmonology and Infection Disease, Yekaterinburg, RussiaDepartment of Microbiology and Preclinical Research, National Medical Research Center of Phthisiopulmonology and Infection Disease, Yekaterinburg, RussiaDepartment of Microbiology and Preclinical Research, National Medical Research Center of Phthisiopulmonology and Infection Disease, Yekaterinburg, RussiaDepartment of Microbiology and Preclinical Research, National Medical Research Center of Phthisiopulmonology and Infection Disease, Yekaterinburg, RussiaDepartment of Microbiology and Preclinical Research, National Medical Research Center of Phthisiopulmonology and Infection Disease, Yekaterinburg, RussiaDepartment of Microbiology and Preclinical Research, National Medical Research Center of Phthisiopulmonology and Infection Disease, Yekaterinburg, RussiaAdministration, National Medical Research Center of Phthisiopulmonology and Infection Disease, Moscow, RussiaABSTRACTCollecting data on rare Mycobacterium tuberculosis (Mtb) clinical isolates with resistance to the new anti-tuberculosis drug bedaquiline is an important task for improving antimicrobial susceptibility testing methods. Nanopore whole genome sequencing, the proportion method on Middlebrook 7H11 medium, and BACTEC MGIT 960 assays were used to analyze genotypic and phenotypic resistance to bedaquiline. We found four mutations: atpE I66M, atpE А63Р, Rv0678 А36Т, and Rv0678 S53P in five isolates with different levels of phenotypic bedaquiline resistance.IMPORTANCEBedaquiline (BDQ) is a new anti-tuberculosis drug. The phenotypic and genotypic data describing the mechanism of drug resistance are critical for the design of rapid and accurate diagnostic tests. We consider that our work, which describes genotypic and phenotypic resistance to BDQ, can contribute to the standardization of drug susceptibility testing.https://journals.asm.org/doi/10.1128/spectrum.03749-23M. tuberculosismutationsbedaquilinenanopore sequencingdrug susceptibility testing |
| spellingShingle | Tatiana Umpeleva Elena Chetverikova Danila Belyaev Natalya Eremeeva Tatiana Boteva Ludmila Golubeva Diana Vakhrusheva Irina Vasilieva Identification of genetic determinants of bedaquiline resistance in Mycobacterium tuberculosis in Ural region, Russia Microbiology Spectrum M. tuberculosis mutations bedaquiline nanopore sequencing drug susceptibility testing |
| title | Identification of genetic determinants of bedaquiline resistance in Mycobacterium tuberculosis in Ural region, Russia |
| title_full | Identification of genetic determinants of bedaquiline resistance in Mycobacterium tuberculosis in Ural region, Russia |
| title_fullStr | Identification of genetic determinants of bedaquiline resistance in Mycobacterium tuberculosis in Ural region, Russia |
| title_full_unstemmed | Identification of genetic determinants of bedaquiline resistance in Mycobacterium tuberculosis in Ural region, Russia |
| title_short | Identification of genetic determinants of bedaquiline resistance in Mycobacterium tuberculosis in Ural region, Russia |
| title_sort | identification of genetic determinants of bedaquiline resistance in mycobacterium tuberculosis in ural region russia |
| topic | M. tuberculosis mutations bedaquiline nanopore sequencing drug susceptibility testing |
| url | https://journals.asm.org/doi/10.1128/spectrum.03749-23 |
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