Assessing undiluted, diluted and frozen-thawed Nili-Ravi buffalo bull sperm by using standard semen assays
Hypo-osmotic swelling test (HOST), eosin-nigrosin staining and normal apical ridge test (NAR) were used to determine integrity of plasma membrane and acrosome of undiluted, diluted (cooled to 5oC) and frozen-thawed sperm. Semen from seven bulls was used. For diluted and frozen-thawed sperm, three do...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2010-02-01
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| Series: | Italian Journal of Animal Science |
| Subjects: | |
| Online Access: | http://www.aspajournal.it/index.php/ijas/article/view/1108 |
| _version_ | 1819174325438119936 |
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| author | A. Ijaz M.I.U.R. Khan |
| author_facet | A. Ijaz M.I.U.R. Khan |
| author_sort | A. Ijaz |
| collection | DOAJ |
| description | Hypo-osmotic swelling test (HOST), eosin-nigrosin staining and normal apical ridge test (NAR) were used to determine integrity of plasma membrane and acrosome of undiluted, diluted (cooled to 5oC) and frozen-thawed sperm. Semen from seven bulls was used. For diluted and frozen-thawed sperm, three doses were pooled at 37oC. Percentage motility was assessed using a phase contrast microscope. A 50μl each of undiluted, diluted and frozen-thawed semen was mixed with 500μl of 50, 100, 150, 150, 190 or 250 mOsm hypo-osmotic treatments of sodium citrate plus fructose and incubated at 37oC for 1 h. Total number of intact sperm (live) of undiluted, diluted and frozen-thawed were assessed before HOST. Percentage motility decreased (P<0.05) among undiluted (81.0 ± 1.57), diluted (69.6 ± 2.24) and frozen thawed (60.1 ± 1.34) sperm. Swellings of plasma membrane of diluted sperm were higher (P<0.05) at 50 and 100 mOsm than undiluted sperm. Similarly, swellings of diluted sperm were higher (P<0.05) than frozen-thawed sperm. Swellings of undiluted sperm were higher (P<0.05) at 100, 150, 190 and 250 mOsm than frozenthawed sperm. Live sperm were higher (P<0.05) in undiluted (174.4 ± 7.33) and diluted (175.6 ± 3.76) as compared to frozen-thawed (142.3 ± 4.84) semen. Integrity of acrosome in undiluted, diluted and frozen-thawed sperm did not differ (P>0.05), but varied significantly (P<0.05) within bulls. In conclusion, plasma membrane integrity of undiluted and diluted sperm was compromised during freezing and thawing. However, freezing had no effect on acrosomal integrity. |
| first_indexed | 2024-12-22T20:37:11Z |
| format | Article |
| id | doaj.art-53ae0cb3428c400cb97e3f3b491b1627 |
| institution | Directory Open Access Journal |
| issn | 1594-4077 1828-051X |
| language | English |
| last_indexed | 2024-12-22T20:37:11Z |
| publishDate | 2010-02-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Italian Journal of Animal Science |
| spelling | doaj.art-53ae0cb3428c400cb97e3f3b491b16272022-12-21T18:13:26ZengTaylor & Francis GroupItalian Journal of Animal Science1594-40771828-051X2010-02-0162s78478710.4081/ijas.2007.s2.784Assessing undiluted, diluted and frozen-thawed Nili-Ravi buffalo bull sperm by using standard semen assaysA. IjazM.I.U.R. KhanHypo-osmotic swelling test (HOST), eosin-nigrosin staining and normal apical ridge test (NAR) were used to determine integrity of plasma membrane and acrosome of undiluted, diluted (cooled to 5oC) and frozen-thawed sperm. Semen from seven bulls was used. For diluted and frozen-thawed sperm, three doses were pooled at 37oC. Percentage motility was assessed using a phase contrast microscope. A 50μl each of undiluted, diluted and frozen-thawed semen was mixed with 500μl of 50, 100, 150, 150, 190 or 250 mOsm hypo-osmotic treatments of sodium citrate plus fructose and incubated at 37oC for 1 h. Total number of intact sperm (live) of undiluted, diluted and frozen-thawed were assessed before HOST. Percentage motility decreased (P<0.05) among undiluted (81.0 ± 1.57), diluted (69.6 ± 2.24) and frozen thawed (60.1 ± 1.34) sperm. Swellings of plasma membrane of diluted sperm were higher (P<0.05) at 50 and 100 mOsm than undiluted sperm. Similarly, swellings of diluted sperm were higher (P<0.05) than frozen-thawed sperm. Swellings of undiluted sperm were higher (P<0.05) at 100, 150, 190 and 250 mOsm than frozenthawed sperm. Live sperm were higher (P<0.05) in undiluted (174.4 ± 7.33) and diluted (175.6 ± 3.76) as compared to frozen-thawed (142.3 ± 4.84) semen. Integrity of acrosome in undiluted, diluted and frozen-thawed sperm did not differ (P>0.05), but varied significantly (P<0.05) within bulls. In conclusion, plasma membrane integrity of undiluted and diluted sperm was compromised during freezing and thawing. However, freezing had no effect on acrosomal integrity.http://www.aspajournal.it/index.php/ijas/article/view/1108Plasma membrane, Undiluted, Diluted semen, Frozen-thawed semen |
| spellingShingle | A. Ijaz M.I.U.R. Khan Assessing undiluted, diluted and frozen-thawed Nili-Ravi buffalo bull sperm by using standard semen assays Italian Journal of Animal Science Plasma membrane, Undiluted, Diluted semen, Frozen-thawed semen |
| title | Assessing undiluted, diluted and frozen-thawed Nili-Ravi buffalo bull sperm by using standard semen assays |
| title_full | Assessing undiluted, diluted and frozen-thawed Nili-Ravi buffalo bull sperm by using standard semen assays |
| title_fullStr | Assessing undiluted, diluted and frozen-thawed Nili-Ravi buffalo bull sperm by using standard semen assays |
| title_full_unstemmed | Assessing undiluted, diluted and frozen-thawed Nili-Ravi buffalo bull sperm by using standard semen assays |
| title_short | Assessing undiluted, diluted and frozen-thawed Nili-Ravi buffalo bull sperm by using standard semen assays |
| title_sort | assessing undiluted diluted and frozen thawed nili ravi buffalo bull sperm by using standard semen assays |
| topic | Plasma membrane, Undiluted, Diluted semen, Frozen-thawed semen |
| url | http://www.aspajournal.it/index.php/ijas/article/view/1108 |
| work_keys_str_mv | AT aijaz assessingundiluteddilutedandfrozenthawedniliravibuffalobullspermbyusingstandardsemenassays AT miurkhan assessingundiluteddilutedandfrozenthawedniliravibuffalobullspermbyusingstandardsemenassays |