Environmental DNA monitoring for short‐term reproductive migration of endemic anadromous species, Shishamo smelt (Spirinchus lanceolatus)
Abstract Monitoring reproductive migration is essential for the conservation of anadromous species. Shishamo smelt (Spirinchus lanceolatus) is endemic to Hokkaido, the northernmost large island in Japan. S. lanceolatus is an anadromous species that is known to migrate into rivers for a very short pe...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2020-04-01
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| Series: | Environmental DNA |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/edn3.50 |
| _version_ | 1819147618764193792 |
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| author | Tetsu Yatsuyanagi Ryotaro Ishida Masayuki K. Sakata Takashi Kanbe Hiroki Mizumoto Yumi Kobayashi Shoko Kamada Satoko Namba Hisaya Nii Toshifumi Minamoto Hitoshi Araki |
| author_facet | Tetsu Yatsuyanagi Ryotaro Ishida Masayuki K. Sakata Takashi Kanbe Hiroki Mizumoto Yumi Kobayashi Shoko Kamada Satoko Namba Hisaya Nii Toshifumi Minamoto Hitoshi Araki |
| author_sort | Tetsu Yatsuyanagi |
| collection | DOAJ |
| description | Abstract Monitoring reproductive migration is essential for the conservation of anadromous species. Shishamo smelt (Spirinchus lanceolatus) is endemic to Hokkaido, the northernmost large island in Japan. S. lanceolatus is an anadromous species that is known to migrate into rivers for a very short period in early winter. While this species has a special value for local fisheries, the catch amount has drastically declined in the last few decades. Information about S. lanceolatus reproductive migration dynamics is limited, which prevents them from being efficiently managed as a resource. In this study, we used environmental DNA (eDNA) methods as a noninvasive molecular tool for estimating presence/absence and abundance/biomass of S. lanceolatus during their migration into rivers. We developed a species‐specific qPCR system for S. lanceolatus, examining (a) temporal variation in S. lanceolatus eDNA concentrations compared with catch data gathered by traditional methods and (b) variability of migratory patterns among river systems. In a core river for their spawning migration, we consistently detected S. lanceolatus eDNA throughout the spawning season, and the temporal distribution of eDNA concentration was consistent with that of the number of migrating S. lanceolatus estimated by catch survey data. In addition, we were able to detect S. lanceolatus eDNA even from rivers without any official record of their migration. Among rivers with eDNA detection, the relative eDNA concentrations varied, indicating that the population biomass differs largely among the river populations. Our study suggests that eDNA detection systems are useful for tracking reproductive migration of S. lanceolatus at fine spatio‐temporal scales. |
| first_indexed | 2024-12-22T13:32:41Z |
| format | Article |
| id | doaj.art-53ae47659bcf42548268126380e7b25c |
| institution | Directory Open Access Journal |
| issn | 2637-4943 |
| language | English |
| last_indexed | 2024-12-22T13:32:41Z |
| publishDate | 2020-04-01 |
| publisher | Wiley |
| record_format | Article |
| series | Environmental DNA |
| spelling | doaj.art-53ae47659bcf42548268126380e7b25c2022-12-21T18:24:09ZengWileyEnvironmental DNA2637-49432020-04-012213013910.1002/edn3.50Environmental DNA monitoring for short‐term reproductive migration of endemic anadromous species, Shishamo smelt (Spirinchus lanceolatus)Tetsu Yatsuyanagi0Ryotaro Ishida1Masayuki K. Sakata2Takashi Kanbe3Hiroki Mizumoto4Yumi Kobayashi5Shoko Kamada6Satoko Namba7Hisaya Nii8Toshifumi Minamoto9Hitoshi Araki10Graduate School of Agriculture Hokkaido University Sapporo JapanHokkaido Research OrganizationFishery Research Department Salmon and Freshwater Fisheries Research Institute Eniwa JapanGraduate School of Human Development and Environment Kobe University Kobe JapanResearch Faculty of Agriculture Hokkaido University Sapporo JapanResearch Faculty of Agriculture Hokkaido University Sapporo JapanResearch Faculty of Agriculture Hokkaido University Sapporo JapanResearch Faculty of Agriculture Hokkaido University Sapporo JapanResearch Faculty of Agriculture Hokkaido University Sapporo JapanHokkaido Aquaculture Promotion Corporation Sapporo JapanGraduate School of Human Development and Environment Kobe University Kobe JapanResearch Faculty of Agriculture Hokkaido University Sapporo JapanAbstract Monitoring reproductive migration is essential for the conservation of anadromous species. Shishamo smelt (Spirinchus lanceolatus) is endemic to Hokkaido, the northernmost large island in Japan. S. lanceolatus is an anadromous species that is known to migrate into rivers for a very short period in early winter. While this species has a special value for local fisheries, the catch amount has drastically declined in the last few decades. Information about S. lanceolatus reproductive migration dynamics is limited, which prevents them from being efficiently managed as a resource. In this study, we used environmental DNA (eDNA) methods as a noninvasive molecular tool for estimating presence/absence and abundance/biomass of S. lanceolatus during their migration into rivers. We developed a species‐specific qPCR system for S. lanceolatus, examining (a) temporal variation in S. lanceolatus eDNA concentrations compared with catch data gathered by traditional methods and (b) variability of migratory patterns among river systems. In a core river for their spawning migration, we consistently detected S. lanceolatus eDNA throughout the spawning season, and the temporal distribution of eDNA concentration was consistent with that of the number of migrating S. lanceolatus estimated by catch survey data. In addition, we were able to detect S. lanceolatus eDNA even from rivers without any official record of their migration. Among rivers with eDNA detection, the relative eDNA concentrations varied, indicating that the population biomass differs largely among the river populations. Our study suggests that eDNA detection systems are useful for tracking reproductive migration of S. lanceolatus at fine spatio‐temporal scales.https://doi.org/10.1002/edn3.50anadromous fishbiomonitoringendemic speciesenvironmental DNAreproductive migration |
| spellingShingle | Tetsu Yatsuyanagi Ryotaro Ishida Masayuki K. Sakata Takashi Kanbe Hiroki Mizumoto Yumi Kobayashi Shoko Kamada Satoko Namba Hisaya Nii Toshifumi Minamoto Hitoshi Araki Environmental DNA monitoring for short‐term reproductive migration of endemic anadromous species, Shishamo smelt (Spirinchus lanceolatus) Environmental DNA anadromous fish biomonitoring endemic species environmental DNA reproductive migration |
| title | Environmental DNA monitoring for short‐term reproductive migration of endemic anadromous species, Shishamo smelt (Spirinchus lanceolatus) |
| title_full | Environmental DNA monitoring for short‐term reproductive migration of endemic anadromous species, Shishamo smelt (Spirinchus lanceolatus) |
| title_fullStr | Environmental DNA monitoring for short‐term reproductive migration of endemic anadromous species, Shishamo smelt (Spirinchus lanceolatus) |
| title_full_unstemmed | Environmental DNA monitoring for short‐term reproductive migration of endemic anadromous species, Shishamo smelt (Spirinchus lanceolatus) |
| title_short | Environmental DNA monitoring for short‐term reproductive migration of endemic anadromous species, Shishamo smelt (Spirinchus lanceolatus) |
| title_sort | environmental dna monitoring for short term reproductive migration of endemic anadromous species shishamo smelt spirinchus lanceolatus |
| topic | anadromous fish biomonitoring endemic species environmental DNA reproductive migration |
| url | https://doi.org/10.1002/edn3.50 |
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