<i>Populus</i><i>PtERF85</i> Balances Xylem Cell Expansion and Secondary Cell Wall Formation in Hybrid Aspen
Secondary growth relies on precise and specialized transcriptional networks that determine cell division, differentiation, and maturation of xylem cells. We identified a novel role for the ethylene-induced <i>Populus Ethylene Response Factor PtERF85</i> (<i>Potri.015G023200</i&g...
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MDPI AG
2021-08-01
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| Online Access: | https://www.mdpi.com/2073-4409/10/8/1971 |
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| author | Carolin Seyfferth Bernard A. Wessels Jorma Vahala Jaakko Kangasjärvi Nicolas Delhomme Torgeir R. Hvidsten Hannele Tuominen Judith Lundberg-Felten |
| author_facet | Carolin Seyfferth Bernard A. Wessels Jorma Vahala Jaakko Kangasjärvi Nicolas Delhomme Torgeir R. Hvidsten Hannele Tuominen Judith Lundberg-Felten |
| author_sort | Carolin Seyfferth |
| collection | DOAJ |
| description | Secondary growth relies on precise and specialized transcriptional networks that determine cell division, differentiation, and maturation of xylem cells. We identified a novel role for the ethylene-induced <i>Populus Ethylene Response Factor PtERF85</i> (<i>Potri.015G023200</i>) in balancing xylem cell expansion and secondary cell wall (SCW) formation in hybrid aspen (<i>Populus tremula x tremuloides</i>). Expression of <i>PtERF85</i> is high in phloem and cambium cells and during the expansion of xylem cells, while it is low in maturing xylem tissue. Extending <i>PtERF85</i> expression into SCW forming zones of woody tissues through ectopic expression reduced wood density and SCW thickness of xylem fibers but increased fiber diameter. Xylem transcriptomes from the transgenic trees revealed transcriptional induction of genes involved in cell expansion, translation, and growth. The expression of genes associated with plant vascular development and the biosynthesis of SCW chemical components such as xylan and lignin, was down-regulated in the transgenic trees. Our results suggest that PtERF85 activates genes related to xylem cell expansion, while preventing transcriptional activation of genes related to SCW formation. The importance of precise spatial expression of <i>PtERF85</i> during wood development together with the observed phenotypes in response to ectopic <i>PtERF85</i> expression suggests that PtERF85 contributes to the transition of fiber cells from elongation to secondary cell wall deposition. |
| first_indexed | 2024-03-10T08:55:27Z |
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| language | English |
| last_indexed | 2024-03-10T08:55:27Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-53af51ead8304283a4487065b95517242023-11-22T07:09:54ZengMDPI AGCells2073-44092021-08-01108197110.3390/cells10081971<i>Populus</i><i>PtERF85</i> Balances Xylem Cell Expansion and Secondary Cell Wall Formation in Hybrid AspenCarolin Seyfferth0Bernard A. Wessels1Jorma Vahala2Jaakko Kangasjärvi3Nicolas Delhomme4Torgeir R. Hvidsten5Hannele Tuominen6Judith Lundberg-Felten7Umeå Plant Science Centre, Department of Plant Physiology, Umeå University, SE-90187 Umeå, SwedenUmeå Plant Science Centre, Department of Plant Physiology, Umeå University, SE-90187 Umeå, SwedenOrganismal and Evolutionary Biology Research Programme, Faculty of Biological and Environmental Sciences, University of Helsinki, FI-00014 Helsinki, FinlandOrganismal and Evolutionary Biology Research Programme, Faculty of Biological and Environmental Sciences, University of Helsinki, FI-00014 Helsinki, FinlandUmeå Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, SE-90184 Umeå, SwedenUmeå Plant Science Centre, Department of Plant Physiology, Umeå University, SE-90187 Umeå, SwedenUmeå Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, SE-90184 Umeå, SwedenUmeå Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, SE-90184 Umeå, SwedenSecondary growth relies on precise and specialized transcriptional networks that determine cell division, differentiation, and maturation of xylem cells. We identified a novel role for the ethylene-induced <i>Populus Ethylene Response Factor PtERF85</i> (<i>Potri.015G023200</i>) in balancing xylem cell expansion and secondary cell wall (SCW) formation in hybrid aspen (<i>Populus tremula x tremuloides</i>). Expression of <i>PtERF85</i> is high in phloem and cambium cells and during the expansion of xylem cells, while it is low in maturing xylem tissue. Extending <i>PtERF85</i> expression into SCW forming zones of woody tissues through ectopic expression reduced wood density and SCW thickness of xylem fibers but increased fiber diameter. Xylem transcriptomes from the transgenic trees revealed transcriptional induction of genes involved in cell expansion, translation, and growth. The expression of genes associated with plant vascular development and the biosynthesis of SCW chemical components such as xylan and lignin, was down-regulated in the transgenic trees. Our results suggest that PtERF85 activates genes related to xylem cell expansion, while preventing transcriptional activation of genes related to SCW formation. The importance of precise spatial expression of <i>PtERF85</i> during wood development together with the observed phenotypes in response to ectopic <i>PtERF85</i> expression suggests that PtERF85 contributes to the transition of fiber cells from elongation to secondary cell wall deposition.https://www.mdpi.com/2073-4409/10/8/1971cell wall thicknessERF85 (CRF4)ligninribosome biogenesiswood developmentxylem expansion |
| spellingShingle | Carolin Seyfferth Bernard A. Wessels Jorma Vahala Jaakko Kangasjärvi Nicolas Delhomme Torgeir R. Hvidsten Hannele Tuominen Judith Lundberg-Felten <i>Populus</i><i>PtERF85</i> Balances Xylem Cell Expansion and Secondary Cell Wall Formation in Hybrid Aspen Cells cell wall thickness ERF85 (CRF4) lignin ribosome biogenesis wood development xylem expansion |
| title | <i>Populus</i><i>PtERF85</i> Balances Xylem Cell Expansion and Secondary Cell Wall Formation in Hybrid Aspen |
| title_full | <i>Populus</i><i>PtERF85</i> Balances Xylem Cell Expansion and Secondary Cell Wall Formation in Hybrid Aspen |
| title_fullStr | <i>Populus</i><i>PtERF85</i> Balances Xylem Cell Expansion and Secondary Cell Wall Formation in Hybrid Aspen |
| title_full_unstemmed | <i>Populus</i><i>PtERF85</i> Balances Xylem Cell Expansion and Secondary Cell Wall Formation in Hybrid Aspen |
| title_short | <i>Populus</i><i>PtERF85</i> Balances Xylem Cell Expansion and Secondary Cell Wall Formation in Hybrid Aspen |
| title_sort | i populus i i pterf85 i balances xylem cell expansion and secondary cell wall formation in hybrid aspen |
| topic | cell wall thickness ERF85 (CRF4) lignin ribosome biogenesis wood development xylem expansion |
| url | https://www.mdpi.com/2073-4409/10/8/1971 |
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