Cloning and Expression of <i>ace</i> Gene of <i>Vibrio cholerae</i> in <i>Escherichia coli</i>
Accessory cholera enterotoxin (ace) gene of Vibrio cholerae, amplified by PCR, is cloned into the BamHI-HindIII sites using plasmid pQE30. Expression of ace gene is under the control of T5 promotor. The Escherichia coli strain M15[pREP4]pAce90 (KM 194), containing recombinant plasmid, is an active p...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | Russian |
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Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe”
2012-04-01
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| Series: | Проблемы особо опасных инфекций |
| Subjects: | |
| Online Access: | https://journal.microbe.ru/jour/article/view/792 |
| _version_ | 1826922506195304448 |
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| author | E. V. Monakhova R. V. Pisanov L. M. Verkina A. V. Mironova |
| author_facet | E. V. Monakhova R. V. Pisanov L. M. Verkina A. V. Mironova |
| author_sort | E. V. Monakhova |
| collection | DOAJ |
| description | Accessory cholera enterotoxin (ace) gene of Vibrio cholerae, amplified by PCR, is cloned into the BamHI-HindIII sites using plasmid pQE30. Expression of ace gene is under the control of T5 promotor. The Escherichia coli strain M15[pREP4]pAce90 (KM 194), containing recombinant plasmid, is an active producer of 6His-Ace protein, which possesses biological activity in the models of suckling mice and causes accumulation of fluid in the intestine. The product is contained in inclusion bodies found in the cells of coliform bacterium. The engineered producer strain can be used to obtain Ace preparations with a view to study its significance as pathogenicity factor in cholera vibrio. |
| first_indexed | 2024-03-08T13:44:22Z |
| format | Article |
| id | doaj.art-53b033dcef64431e96cf6f98eb0ab2dd |
| institution | Directory Open Access Journal |
| issn | 0370-1069 2658-719X |
| language | Russian |
| last_indexed | 2025-02-17T14:09:47Z |
| publishDate | 2012-04-01 |
| publisher | Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe” |
| record_format | Article |
| series | Проблемы особо опасных инфекций |
| spelling | doaj.art-53b033dcef64431e96cf6f98eb0ab2dd2024-12-24T12:09:49ZrusFederal Government Health Institution, Russian Research Anti-Plague Institute “Microbe”Проблемы особо опасных инфекций0370-10692658-719X2012-04-0102(112)394210.21055/0370-1069-2012-2(112)-39-42776Cloning and Expression of <i>ace</i> Gene of <i>Vibrio cholerae</i> in <i>Escherichia coli</i>E. V. Monakhova0R. V. Pisanov1L. M. Verkina2A. V. Mironova3Rostov-on-Don Research Anti-Plague InstituteRostov-on-Don Research Anti-Plague InstituteRostov-on-Don Research Anti-Plague InstituteRostov-on-Don Research Anti-Plague InstituteAccessory cholera enterotoxin (ace) gene of Vibrio cholerae, amplified by PCR, is cloned into the BamHI-HindIII sites using plasmid pQE30. Expression of ace gene is under the control of T5 promotor. The Escherichia coli strain M15[pREP4]pAce90 (KM 194), containing recombinant plasmid, is an active producer of 6His-Ace protein, which possesses biological activity in the models of suckling mice and causes accumulation of fluid in the intestine. The product is contained in inclusion bodies found in the cells of coliform bacterium. The engineered producer strain can be used to obtain Ace preparations with a view to study its significance as pathogenicity factor in cholera vibrio.https://journal.microbe.ru/jour/article/view/792accessory cholera enterotoxincholera vibriogene cloningrecombinant plasmid |
| spellingShingle | E. V. Monakhova R. V. Pisanov L. M. Verkina A. V. Mironova Cloning and Expression of <i>ace</i> Gene of <i>Vibrio cholerae</i> in <i>Escherichia coli</i> Проблемы особо опасных инфекций accessory cholera enterotoxin cholera vibrio gene cloning recombinant plasmid |
| title | Cloning and Expression of <i>ace</i> Gene of <i>Vibrio cholerae</i> in <i>Escherichia coli</i> |
| title_full | Cloning and Expression of <i>ace</i> Gene of <i>Vibrio cholerae</i> in <i>Escherichia coli</i> |
| title_fullStr | Cloning and Expression of <i>ace</i> Gene of <i>Vibrio cholerae</i> in <i>Escherichia coli</i> |
| title_full_unstemmed | Cloning and Expression of <i>ace</i> Gene of <i>Vibrio cholerae</i> in <i>Escherichia coli</i> |
| title_short | Cloning and Expression of <i>ace</i> Gene of <i>Vibrio cholerae</i> in <i>Escherichia coli</i> |
| title_sort | cloning and expression of i ace i gene of i vibrio cholerae i in i escherichia coli i |
| topic | accessory cholera enterotoxin cholera vibrio gene cloning recombinant plasmid |
| url | https://journal.microbe.ru/jour/article/view/792 |
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