A Novel NIR-FRET Biosensor for Reporting PS/γ-Secretase Activity in Live Cells

A Novel NIR-FRET Biosensor for Reporting PS/γ-Secretase Activity in Live Cells

Presenilin (PS)/γ-secretase plays a pivotal role in essential cellular events via proteolytic processing of transmembrane proteins that include APP and Notch receptors. However, how PS/γ-secretase activity is spatiotemporally regulated by other molecular and cellular factors and how the changes in P...

Full description

Bibliographic Details
Main Authors:	Mei CQ Houser, Steven S Hou, Florian Perrin, Yuliia Turchyna, Brian J Bacskai, Oksana Berezovska, Masato Maesako
Format:	Article
Language:	English
Published:	MDPI AG 2020-10-01
Series:	Sensors
Subjects:	Presenilin (PS)/γ-secretase FRET biosensor near-infrared (NIR) miRFP670 and 720
Online Access:	https://www.mdpi.com/1424-8220/20/21/5980

Similar Items

Limited Substrate Specificity of PS/γ-Secretase Is Supported by Novel Multiplexed FRET Analysis in Live Cells
by: Mei C. Q. Houser, et al.
Published: (2021-05-01)

The Potential of Gamma Secretase as a Therapeutic Target for Cardiac Diseases
by: Sujoita Sen, et al.
Published: (2021-12-01)

Functional γ-secretase complex assembly in Golgi/trans-Golgi network: interactions among presenilin, nicastrin, Aph1, Pen-2, and γ-secretase substrates
by: Stephanie Baulac, et al.
Published: (2003-11-01)

Conserved “PAL” sequence in presenilins is essential for γ-secretase activity, but not required for formation or stabilization of γ-secretase complexes
by: Jun Wang, et al.
Published: (2004-04-01)

Rapid Biodistribution of Fluorescent Outer-Membrane Vesicles from the Intestine to Distant Organs via the Blood in Mice
by: Béatrice Schaack, et al.
Published: (2024-02-01)

Overexpression of N141I PS2 increases γ-secretase activity through up-regulation of Presenilin and Pen-2 in brain mitochondria of NSE/hPS2m transgenic mice
by: Woo Bin Yun, et al.
Published: (2016-12-01)

Presenilins and γ-Secretase in Membrane Proteostasis
by: Naoto Oikawa, et al.
Published: (2019-03-01)

Complex Regulation of gamma-Secretase: from Obligatory to Modulatory Subunits
by: Natalya eGertsik, et al.
Published: (2015-01-01)

How γ-secretase hits a moving target
by: Charles R Sanders
Published: (2016-08-01)

Endosome and Lysosome Membrane Properties Functionally Link to γ-Secretase in Live/Intact Cells
by: Mei C. Q. Houser, et al.
Published: (2023-02-01)

γ-Secretase modulators exhibit selectivity for modulation of APP cleavage but inverse γ-secretase modulators do not
by: Christian B. Lessard, et al.
Published: (2020-05-01)

Non-Invasive Fluorescent Monitoring of Ovarian Cancer in an Immunocompetent Mouse Model
by: Amy L. Wilson, et al.
Published: (2018-12-01)

Pathological activity of familial Alzheimer’s disease-associated mutant presenilin can be executed by six different γ-secretase complexes
by: Keiro Shirotani, et al.
Published: (2007-07-01)

Super-resolution microscopy reveals majorly mono- and dimeric presenilin1/γ-secretase at the cell surface
by: Abril Angélica Escamilla-Ayala, et al.
Published: (2020-07-01)

Substrate–Enzyme Interactions in Intramembrane Proteolysis: γ-Secretase as the Prototype
by: Xinyue Liu, et al.
Published: (2020-05-01)

Pathogenic PS1 phosphorylation at Ser367
by: Masato Maesako, et al.
Published: (2017-01-01)

Clues to γ-secretase, huntingtin and Hirano body normal function using the model organism <it>Dictyostelium discoideum</it>
by: Myre Michael A
Published: (2012-04-01)

Analysis of Non-Amyloidogenic Mutations in APP Supports Loss of Function Hypothesis of Alzheimer’s Disease
by: Meewhi Kim, et al.
Published: (2023-01-01)

In-Depth Characterization of Endo-Lysosomal Aβ in Intact Neurons
by: Alec K. McKendell, et al.
Published: (2022-08-01)

Loss of presenilin function enhances tau phosphorylation and aggregation in mice
by: Carlos M. Soto-Faguás, et al.
Published: (2021-09-01)

Presenilin/γ-secretase-dependent EphA3 processing mediates axon elongation through non-muscle myosin IIA
by: Míriam Javier-Torrent, et al.
Published: (2019-10-01)

Design and Initial Characterization of a Small Near-Infrared Fluorescent Calcium Indicator
by: Mikhail E. Matlashov, et al.
Published: (2022-06-01)

Jagged1 protein processing in the developing mammalian lens
by: Mina Azimi, et al.
Published: (2019-03-01)

Expression of Amyloid Precursor Protein, Caveolin-1, Alpha-, Beta-, and Gamma-Secretases in Penumbra Cells after Photothrombotic Stroke and Evaluation of Neuroprotective Effect of Secretase and Caveolin-1 Inhibitors
by: Svetlana Sharifulina, et al.
Published: (2022-10-01)

Celastrol and Triptolide Suppress Stemness in Triple Negative Breast Cancer: Notch as a Therapeutic Target for Stem Cells
by: Prabhu Ramamoorthy, et al.
Published: (2021-04-01)

Participation of Amyloid and Tau Protein in Neuronal Death and Neurodegeneration after Brain Ischemia
by: Ryszard Pluta, et al.
Published: (2020-06-01)

Active site geometry stabilization of a presenilin homolog by the lipid bilayer promotes intramembrane proteolysis
by: Lukas P Feilen, et al.
Published: (2022-05-01)

Localization and regional distribution of p23/TMP21 in the brain
by: Kulandaivelu S. Vetrivel, et al.
Published: (2008-10-01)

Excess of nicastrin in brain results in heterozygosity having no effect on endogenous APP processing and amyloid peptide levels in vivo
by: Sonya Brijbassi, et al.
Published: (2007-02-01)

SYNTHESIS OF MECHANISMS OF FRETTING PHENOMEN
by: Stefan GHIMISI
Published: (2018-05-01)

Multimodal cell tracking from systemic administration to tumour growth by combining gold nanorods and reporter genes
by: Joan Comenge, et al.
Published: (2018-06-01)

Dendrobium alkaloids decrease Aβ by regulating α- and β-secretases in hippocampal neurons of SD rats
by: Juan Huang, et al.
Published: (2019-09-01)

Pharmacogenetic Study on the Effect of Rivastigmine on PS2 and APOE Genes in Iranian Alzheimer Patients
by: M. Zamani, et al.
Published: (2011-06-01)

Regulated intramembrane proteolysis, innate immunity and therapeutic targets in Alzheimer’s disease
by: Caroline Coleman-Vaughan, et al.
Published: (2016-04-01)

Brain Ischemia as a Prelude to Alzheimer's Disease
by: Ryszard Pluta, et al.
Published: (2021-02-01)

Characterization of the ER-Targeted Low Affinity Ca2+ Probe D4ER
by: Elisa Greotti, et al.
Published: (2016-09-01)

TEST STANDS AND METHODS FOR INVESTIGATING THE FRETTING PHENOMENON
by: Stefan Ghimisi
Published: (2018-12-01)

Gamma-secretase modulators: a promising route for the treatment of Alzheimer's disease
by: Gunnar Nordvall, et al.
Published: (2023-10-01)

Comparative Analysis of Bacteriophytochrome Agp2 and Its Engineered Photoactivatable NIR Fluorescent Proteins PAiRFP1 and PAiRFP2
by: Faez Iqbal Khan, et al.
Published: (2020-09-01)

Soluble Gamma-secretase Modulators Attenuate Alzheimer's β-amyloid Pathology and Induce Conformational Changes in Presenilin 1
by: Frank Raven, et al.
Published: (2017-10-01)