FACTORS AFFECTING THE (PRODUCTION OF EXTRACELLULAR NEUTRAL PROTEASE) BY STREPTOMYCES MICROFLAVUS
In order to produce neutral protease from Streptomyces microflavus, it was cultivated in basal medium containing soluble starch, potassium nitrate, dipotassium hydrogen phosphate, magnesium sulphate, sodium chloride, calcium carbonate and ferrous sulphate. Protease production increased with decreasi...
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| Format: | Article |
| Language: | Arabic |
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The Union of Arab Universities
2009-03-01
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| Series: | Arab Universities Journal of Agricultural Sciences |
| Subjects: | |
| Online Access: | https://ajs.journals.ekb.eg/article_14547_0180ce509f70ebdf7a661ea4fb6c169d.pdf |
| _version_ | 1827323451448229888 |
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| author | Saadia, M. Hassanein Hala M. Rifaat Osama Hamed El-Said Souad A. Saleh Manal S. M. Selim |
| author_facet | Saadia, M. Hassanein Hala M. Rifaat Osama Hamed El-Said Souad A. Saleh Manal S. M. Selim |
| author_sort | Saadia, M. Hassanein |
| collection | DOAJ |
| description | In order to produce neutral protease from Streptomyces microflavus, it was cultivated in basal medium containing soluble starch, potassium nitrate, dipotassium hydrogen phosphate, magnesium sulphate, sodium chloride, calcium carbonate and ferrous sulphate. Protease production increased with decreasing the ratio of culture broth to vessel volume 1:5 and shaking at 150 rpm. Protease production was low when pH was < 5 or > 9. The productivity of protease decreased sharply when the incubation temperature increased from 30 to 450C. The maximum yield of protease was obtained at the third day of incubation. Soluble starch and yeast extract were the best carbon and nitrogen sources, respectively. Supplementation with calcium carbonate enhanced protease production. In addition, the dipotassium hydrogen phosphate was the best phosphorous source. Para chloromercuribenzoic acid and phenyl methylsulfonylfloride had significant inhibitory effect on protease production. |
| first_indexed | 2024-04-25T01:51:54Z |
| format | Article |
| id | doaj.art-53b0ff847fe94f0fbb7cc305eecfad0f |
| institution | Directory Open Access Journal |
| issn | 1110-2675 2636-3585 |
| language | Arabic |
| last_indexed | 2024-04-25T01:51:54Z |
| publishDate | 2009-03-01 |
| publisher | The Union of Arab Universities |
| record_format | Article |
| series | Arab Universities Journal of Agricultural Sciences |
| spelling | doaj.art-53b0ff847fe94f0fbb7cc305eecfad0f2024-03-07T17:45:44ZaraThe Union of Arab UniversitiesArab Universities Journal of Agricultural Sciences1110-26752636-35852009-03-01171334110.21608/ajs.2009.1454714547FACTORS AFFECTING THE (PRODUCTION OF EXTRACELLULAR NEUTRAL PROTEASE) BY STREPTOMYCES MICROFLAVUSSaadia, M. Hassanein0Hala M. Rifaat1Osama Hamed El-Said2Souad A. Saleh3Manal S. M. Selim4- Microbiology Department, Ain Shams University, Abbasiya,Cairo, Egypt- Microbial Chemistry Department, National Research Centre, Dokki, Cairo, EgyptMicrobial Biotechnology Department, National Research Centre, Dokki, Cairo, EgyptMicrobial Biotechnology Department, National Research Centre, Dokki, Cairo, EgyptMicrobial Biotechnology Department, National Research Centre, Dokki, Cairo, EgyptIn order to produce neutral protease from Streptomyces microflavus, it was cultivated in basal medium containing soluble starch, potassium nitrate, dipotassium hydrogen phosphate, magnesium sulphate, sodium chloride, calcium carbonate and ferrous sulphate. Protease production increased with decreasing the ratio of culture broth to vessel volume 1:5 and shaking at 150 rpm. Protease production was low when pH was < 5 or > 9. The productivity of protease decreased sharply when the incubation temperature increased from 30 to 450C. The maximum yield of protease was obtained at the third day of incubation. Soluble starch and yeast extract were the best carbon and nitrogen sources, respectively. Supplementation with calcium carbonate enhanced protease production. In addition, the dipotassium hydrogen phosphate was the best phosphorous source. Para chloromercuribenzoic acid and phenyl methylsulfonylfloride had significant inhibitory effect on protease production.https://ajs.journals.ekb.eg/article_14547_0180ce509f70ebdf7a661ea4fb6c169d.pdfstreptomycetes microflavuscultivationproductionprotease enzyme |
| spellingShingle | Saadia, M. Hassanein Hala M. Rifaat Osama Hamed El-Said Souad A. Saleh Manal S. M. Selim FACTORS AFFECTING THE (PRODUCTION OF EXTRACELLULAR NEUTRAL PROTEASE) BY STREPTOMYCES MICROFLAVUS Arab Universities Journal of Agricultural Sciences streptomycetes microflavus cultivation production protease enzyme |
| title | FACTORS AFFECTING THE (PRODUCTION OF EXTRACELLULAR NEUTRAL PROTEASE) BY STREPTOMYCES MICROFLAVUS |
| title_full | FACTORS AFFECTING THE (PRODUCTION OF EXTRACELLULAR NEUTRAL PROTEASE) BY STREPTOMYCES MICROFLAVUS |
| title_fullStr | FACTORS AFFECTING THE (PRODUCTION OF EXTRACELLULAR NEUTRAL PROTEASE) BY STREPTOMYCES MICROFLAVUS |
| title_full_unstemmed | FACTORS AFFECTING THE (PRODUCTION OF EXTRACELLULAR NEUTRAL PROTEASE) BY STREPTOMYCES MICROFLAVUS |
| title_short | FACTORS AFFECTING THE (PRODUCTION OF EXTRACELLULAR NEUTRAL PROTEASE) BY STREPTOMYCES MICROFLAVUS |
| title_sort | factors affecting the production of extracellular neutral protease by streptomyces microflavus |
| topic | streptomycetes microflavus cultivation production protease enzyme |
| url | https://ajs.journals.ekb.eg/article_14547_0180ce509f70ebdf7a661ea4fb6c169d.pdf |
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