The effect of time and temperature on viability and performance of Langerhans islets separated from Balb/c mouse after death
Background: Tissue transplantation plays a pivotal role in the treatment of diseases. Pancreatic beta cell transplantation is the best way to obtain normal blood glucose in patients with diabetes type 1. However, it is not clear how long endocrine pancreas cells can be used for transplantation after...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2015-01-01
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| Series: | Advanced Biomedical Research |
| Subjects: | |
| Online Access: | http://www.advbiores.net/article.asp?issn=2277-9175;year=2015;volume=4;issue=1;spage=93;epage=93;aulast=Ghorbani |
| _version_ | 1818176662675652608 |
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| author | Rostam Ghorbani Cyrus Jalili Mohammad Reza Salahshoor Maryam Shiasi |
| author_facet | Rostam Ghorbani Cyrus Jalili Mohammad Reza Salahshoor Maryam Shiasi |
| author_sort | Rostam Ghorbani |
| collection | DOAJ |
| description | Background: Tissue transplantation plays a pivotal role in the treatment of diseases. Pancreatic beta cell transplantation is the best way to obtain normal blood glucose in patients with diabetes type 1. However, it is not clear how long endocrine pancreas cells can be used for transplantation after the donor′s death. The present study was conducted to analyze the performance and viability of pancreatic islet cells after death.
Materials and Methods: Pancreas was separated from Balb/c mice at different times (0, 1, 4, 6, 12, and 24 h after death) at temperatures of 4°C and 23°C, and was cultured in Roswell_Park_Memorial_Institute (RPMI) 1640. Insulin shock, MTT assay, aldehyde fuchsin staining, dithizone staining, and florescence microscopy methods were applied to analyze the performance of beta cells, cell viability, islets′ diagnosis, islet cells′ diagnosis, and viable and necrotic cells diagnosis, respectively.
Results: Islets of Langerhans and beta cells were diagnosed. By increasing the temperature and time, the viability and performance of beta cells decreased significantly (P < 0.05).
Conclusion: The best condition for keeping the islets of Langerhans in terms of viability and performance is 4 h after death at temperature of 4°C. |
| first_indexed | 2024-12-11T20:19:45Z |
| format | Article |
| id | doaj.art-53b164c7ef6b4eb7adce71e6ac71668f |
| institution | Directory Open Access Journal |
| issn | 2277-9175 2277-9175 |
| language | English |
| last_indexed | 2024-12-11T20:19:45Z |
| publishDate | 2015-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Advanced Biomedical Research |
| spelling | doaj.art-53b164c7ef6b4eb7adce71e6ac71668f2022-12-22T00:52:06ZengWolters Kluwer Medknow PublicationsAdvanced Biomedical Research2277-91752277-91752015-01-0141939310.4103/2277-9175.156657The effect of time and temperature on viability and performance of Langerhans islets separated from Balb/c mouse after deathRostam GhorbaniCyrus JaliliMohammad Reza SalahshoorMaryam ShiasiBackground: Tissue transplantation plays a pivotal role in the treatment of diseases. Pancreatic beta cell transplantation is the best way to obtain normal blood glucose in patients with diabetes type 1. However, it is not clear how long endocrine pancreas cells can be used for transplantation after the donor′s death. The present study was conducted to analyze the performance and viability of pancreatic islet cells after death. Materials and Methods: Pancreas was separated from Balb/c mice at different times (0, 1, 4, 6, 12, and 24 h after death) at temperatures of 4°C and 23°C, and was cultured in Roswell_Park_Memorial_Institute (RPMI) 1640. Insulin shock, MTT assay, aldehyde fuchsin staining, dithizone staining, and florescence microscopy methods were applied to analyze the performance of beta cells, cell viability, islets′ diagnosis, islet cells′ diagnosis, and viable and necrotic cells diagnosis, respectively. Results: Islets of Langerhans and beta cells were diagnosed. By increasing the temperature and time, the viability and performance of beta cells decreased significantly (P < 0.05). Conclusion: The best condition for keeping the islets of Langerhans in terms of viability and performance is 4 h after death at temperature of 4°C.http://www.advbiores.net/article.asp?issn=2277-9175;year=2015;volume=4;issue=1;spage=93;epage=93;aulast=GhorbaniCell viabilityLangerhans isletsperformancetemperaturetime |
| spellingShingle | Rostam Ghorbani Cyrus Jalili Mohammad Reza Salahshoor Maryam Shiasi The effect of time and temperature on viability and performance of Langerhans islets separated from Balb/c mouse after death Advanced Biomedical Research Cell viability Langerhans islets performance temperature time |
| title | The effect of time and temperature on viability and performance of Langerhans islets separated from Balb/c mouse after death |
| title_full | The effect of time and temperature on viability and performance of Langerhans islets separated from Balb/c mouse after death |
| title_fullStr | The effect of time and temperature on viability and performance of Langerhans islets separated from Balb/c mouse after death |
| title_full_unstemmed | The effect of time and temperature on viability and performance of Langerhans islets separated from Balb/c mouse after death |
| title_short | The effect of time and temperature on viability and performance of Langerhans islets separated from Balb/c mouse after death |
| title_sort | effect of time and temperature on viability and performance of langerhans islets separated from balb c mouse after death |
| topic | Cell viability Langerhans islets performance temperature time |
| url | http://www.advbiores.net/article.asp?issn=2277-9175;year=2015;volume=4;issue=1;spage=93;epage=93;aulast=Ghorbani |
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