Genome Characterisation of an Isoprene-Degrading <i>Alcaligenes</i> sp. Isolated from a Tropical Restored Forest
Isoprene is a climate-active biogenic volatile organic compound (BVOC), emitted into the atmosphere in abundance, mainly from terrestrial plants. Soil is an important sink for isoprene due to its consumption by microbes. In this study, we report the ability of a soil bacterium to degrade isoprene. S...
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MDPI AG
2022-03-01
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| Online Access: | https://www.mdpi.com/2079-7737/11/4/519 |
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| author | Toungporn Uttarotai Sawannee Sutheeworapong Andrew T. Crombie J. Colin Murrell Wuttichai Mhuantong Nuttapol Noirungsee Sunanta Wangkarn Sakunnee Bovonsombut Terry J. McGenity Thararat Chitov |
| author_facet | Toungporn Uttarotai Sawannee Sutheeworapong Andrew T. Crombie J. Colin Murrell Wuttichai Mhuantong Nuttapol Noirungsee Sunanta Wangkarn Sakunnee Bovonsombut Terry J. McGenity Thararat Chitov |
| author_sort | Toungporn Uttarotai |
| collection | DOAJ |
| description | Isoprene is a climate-active biogenic volatile organic compound (BVOC), emitted into the atmosphere in abundance, mainly from terrestrial plants. Soil is an important sink for isoprene due to its consumption by microbes. In this study, we report the ability of a soil bacterium to degrade isoprene. Strain 13f was isolated from soil beneath wild Himalayan cherry trees in a tropical restored forest. Based on phylogenomic analysis and an Average Nucleotide Identity score of >95%, it most probably belongs to the species <i>Alcaligenes faecalis</i>. Isoprene degradation by <i>Alcaligenes</i> sp. strain 13f was measured by using gas chromatography. When isoprene was supplied as the sole carbon and energy source at the concentration of 7.2 × 10<sup>5</sup> ppbv and 7.2 × 10<sup>6</sup> ppbv, 32.6% and 19.6% of isoprene was consumed after 18 days, respectively. Genome analysis of <i>Alcaligenes</i> sp. strain 13f revealed that the genes that are typically found as part of the isoprene monooxygenase gene cluster in other isoprene-degrading bacteria were absent. This discovery suggests that there may be alternative pathways for isoprene metabolism. |
| first_indexed | 2024-03-09T11:08:59Z |
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| id | doaj.art-53b23ca5207f4814b3032843a31c9ad4 |
| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-09T11:08:59Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology |
| spelling | doaj.art-53b23ca5207f4814b3032843a31c9ad42023-12-01T00:50:51ZengMDPI AGBiology2079-77372022-03-0111451910.3390/biology11040519Genome Characterisation of an Isoprene-Degrading <i>Alcaligenes</i> sp. Isolated from a Tropical Restored ForestToungporn Uttarotai0Sawannee Sutheeworapong1Andrew T. Crombie2J. Colin Murrell3Wuttichai Mhuantong4Nuttapol Noirungsee5Sunanta Wangkarn6Sakunnee Bovonsombut7Terry J. McGenity8Thararat Chitov9Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, ThailandSchool of Bioresources and Technology, King Mongkut’s University of Technology Thonburi, Bangkok 10150, ThailandSchool of Environmental Sciences, University of East Anglia, Norwich NR4 7TJ, UKSchool of Environmental Sciences, University of East Anglia, Norwich NR4 7TJ, UKEnzyme Technology Research Team, Biorefinery and Bioproduct Technology Research Group, National Center for Genetic Engineering and Biotechnology, Pathumthani 12120, ThailandDepartment of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, ThailandDepartment of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, ThailandDepartment of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, ThailandSchool of Life Sciences, University of Essex, Colchester CO4 3SQ, UKDepartment of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, ThailandIsoprene is a climate-active biogenic volatile organic compound (BVOC), emitted into the atmosphere in abundance, mainly from terrestrial plants. Soil is an important sink for isoprene due to its consumption by microbes. In this study, we report the ability of a soil bacterium to degrade isoprene. Strain 13f was isolated from soil beneath wild Himalayan cherry trees in a tropical restored forest. Based on phylogenomic analysis and an Average Nucleotide Identity score of >95%, it most probably belongs to the species <i>Alcaligenes faecalis</i>. Isoprene degradation by <i>Alcaligenes</i> sp. strain 13f was measured by using gas chromatography. When isoprene was supplied as the sole carbon and energy source at the concentration of 7.2 × 10<sup>5</sup> ppbv and 7.2 × 10<sup>6</sup> ppbv, 32.6% and 19.6% of isoprene was consumed after 18 days, respectively. Genome analysis of <i>Alcaligenes</i> sp. strain 13f revealed that the genes that are typically found as part of the isoprene monooxygenase gene cluster in other isoprene-degrading bacteria were absent. This discovery suggests that there may be alternative pathways for isoprene metabolism.https://www.mdpi.com/2079-7737/11/4/519isopreneclimate-active gasisoprene degradationgenome<i>Alcaligenes</i> |
| spellingShingle | Toungporn Uttarotai Sawannee Sutheeworapong Andrew T. Crombie J. Colin Murrell Wuttichai Mhuantong Nuttapol Noirungsee Sunanta Wangkarn Sakunnee Bovonsombut Terry J. McGenity Thararat Chitov Genome Characterisation of an Isoprene-Degrading <i>Alcaligenes</i> sp. Isolated from a Tropical Restored Forest Biology isoprene climate-active gas isoprene degradation genome <i>Alcaligenes</i> |
| title | Genome Characterisation of an Isoprene-Degrading <i>Alcaligenes</i> sp. Isolated from a Tropical Restored Forest |
| title_full | Genome Characterisation of an Isoprene-Degrading <i>Alcaligenes</i> sp. Isolated from a Tropical Restored Forest |
| title_fullStr | Genome Characterisation of an Isoprene-Degrading <i>Alcaligenes</i> sp. Isolated from a Tropical Restored Forest |
| title_full_unstemmed | Genome Characterisation of an Isoprene-Degrading <i>Alcaligenes</i> sp. Isolated from a Tropical Restored Forest |
| title_short | Genome Characterisation of an Isoprene-Degrading <i>Alcaligenes</i> sp. Isolated from a Tropical Restored Forest |
| title_sort | genome characterisation of an isoprene degrading i alcaligenes i sp isolated from a tropical restored forest |
| topic | isoprene climate-active gas isoprene degradation genome <i>Alcaligenes</i> |
| url | https://www.mdpi.com/2079-7737/11/4/519 |
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