Effects of Angiotensin II on Erythropoietin Production in the Kidney and Liver
The kidney is a main site of erythropoietin production in the body. We developed a new method for the detection of Epo protein by deglycosylation-coupled Western blotting. Detection of deglycosylated Epo enables the examination of small changes in Epo production. Using this method, we investigated t...
| Main Authors: | , , , , , , , , , , , , , , |
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MDPI AG
2021-09-01
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| Online Access: | https://www.mdpi.com/1420-3049/26/17/5399 |
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| author | Yukiko Yasuoka Yuichiro Izumi Takashi Fukuyama Hideki Inoue Tomomi Oshima Taiga Yamazaki Takayuki Uematsu Noritada Kobayashi Yoshitaka Shimada Yasushi Nagaba Masashi Mukoyama Yuichi Sato Jeff M Sands Katsumasa Kawahara Hiroshi Nonoguchi |
| author_facet | Yukiko Yasuoka Yuichiro Izumi Takashi Fukuyama Hideki Inoue Tomomi Oshima Taiga Yamazaki Takayuki Uematsu Noritada Kobayashi Yoshitaka Shimada Yasushi Nagaba Masashi Mukoyama Yuichi Sato Jeff M Sands Katsumasa Kawahara Hiroshi Nonoguchi |
| author_sort | Yukiko Yasuoka |
| collection | DOAJ |
| description | The kidney is a main site of erythropoietin production in the body. We developed a new method for the detection of Epo protein by deglycosylation-coupled Western blotting. Detection of deglycosylated Epo enables the examination of small changes in Epo production. Using this method, we investigated the effects of angiotensin II (ATII) on Epo production in the kidney. ATII stimulated the plasma Epo concentration; Epo, HIF2α, and PHD2 mRNA expression in nephron segments in the renal cortex and outer medulla; and Epo protein expression in the renal cortex. In situ hybridization and immunohistochemistry revealed that ATII stimulates Epo mRNA and protein expression not only in proximal tubules but also in collecting ducts, especially in intercalated cells. These data support the regulation of Epo production in the kidney by the renin–angiotensin–aldosterone system (RAS). |
| first_indexed | 2024-03-10T08:05:49Z |
| format | Article |
| id | doaj.art-53b4ec3a22984384821c45b5bf2f1e37 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T08:05:49Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-53b4ec3a22984384821c45b5bf2f1e372023-11-22T11:03:35ZengMDPI AGMolecules1420-30492021-09-012617539910.3390/molecules26175399Effects of Angiotensin II on Erythropoietin Production in the Kidney and LiverYukiko Yasuoka0Yuichiro Izumi1Takashi Fukuyama2Hideki Inoue3Tomomi Oshima4Taiga Yamazaki5Takayuki Uematsu6Noritada Kobayashi7Yoshitaka Shimada8Yasushi Nagaba9Masashi Mukoyama10Yuichi Sato11Jeff M Sands12Katsumasa Kawahara13Hiroshi Nonoguchi14Department of Physiology, Kitasato University School of Medicine, 1-15-1 Kitasato, Minami-ku, Sagamihara 252-0374, Kanagawa, JapanDepartment of Nephrology, Graduate School of Medical Sciences, Kumamoto University, 1-1-1 Honjo, Chuo-ku, Kumamoto 860-8556, Kumamoto, JapanDivision of Biomedical Research, Kitasato University Medical Center, 6-100 Arai, Kitamoto 364-8501, Saitama, JapanDepartment of Nephrology, Graduate School of Medical Sciences, Kumamoto University, 1-1-1 Honjo, Chuo-ku, Kumamoto 860-8556, Kumamoto, JapanDepartment of Physiology, Kitasato University School of Medicine, 1-15-1 Kitasato, Minami-ku, Sagamihara 252-0374, Kanagawa, JapanDivision of Biomedical Research, Kitasato University Medical Center, 6-100 Arai, Kitamoto 364-8501, Saitama, JapanDivision of Biomedical Research, Kitasato University Medical Center, 6-100 Arai, Kitamoto 364-8501, Saitama, JapanDivision of Biomedical Research, Kitasato University Medical Center, 6-100 Arai, Kitamoto 364-8501, Saitama, JapanDivision of Internal Medicine, Kitasato University Medical Center, 6-100 Arai, Kitamoto 364-8501, Saitama, JapanDivision of Internal Medicine, Kitasato University Medical Center, 6-100 Arai, Kitamoto 364-8501, Saitama, JapanDepartment of Nephrology, Graduate School of Medical Sciences, Kumamoto University, 1-1-1 Honjo, Chuo-ku, Kumamoto 860-8556, Kumamoto, JapanDepartment of Molecular Diagnostics, School of Allied Health Sciences, Kitasato University, 1-15-1 Kitasato, Minami-ku, Sagamihara 252-0373, Kanagawa, JapanRenal Division, Department of Medicine, Emory University School of Medicine, 1639 Pierce Drive, WMB Room 3313, Atlanta, GA 30322, USADepartment of Physiology, Kitasato University School of Medicine, 1-15-1 Kitasato, Minami-ku, Sagamihara 252-0374, Kanagawa, JapanDivision of Internal Medicine, Kitasato University Medical Center, 6-100 Arai, Kitamoto 364-8501, Saitama, JapanThe kidney is a main site of erythropoietin production in the body. We developed a new method for the detection of Epo protein by deglycosylation-coupled Western blotting. Detection of deglycosylated Epo enables the examination of small changes in Epo production. Using this method, we investigated the effects of angiotensin II (ATII) on Epo production in the kidney. ATII stimulated the plasma Epo concentration; Epo, HIF2α, and PHD2 mRNA expression in nephron segments in the renal cortex and outer medulla; and Epo protein expression in the renal cortex. In situ hybridization and immunohistochemistry revealed that ATII stimulates Epo mRNA and protein expression not only in proximal tubules but also in collecting ducts, especially in intercalated cells. These data support the regulation of Epo production in the kidney by the renin–angiotensin–aldosterone system (RAS).https://www.mdpi.com/1420-3049/26/17/5399erythropoietinangiotensin IIrenin–angiotensin–aldosterone systemdeglycosylationWestern blottingHIF2α |
| spellingShingle | Yukiko Yasuoka Yuichiro Izumi Takashi Fukuyama Hideki Inoue Tomomi Oshima Taiga Yamazaki Takayuki Uematsu Noritada Kobayashi Yoshitaka Shimada Yasushi Nagaba Masashi Mukoyama Yuichi Sato Jeff M Sands Katsumasa Kawahara Hiroshi Nonoguchi Effects of Angiotensin II on Erythropoietin Production in the Kidney and Liver Molecules erythropoietin angiotensin II renin–angiotensin–aldosterone system deglycosylation Western blotting HIF2α |
| title | Effects of Angiotensin II on Erythropoietin Production in the Kidney and Liver |
| title_full | Effects of Angiotensin II on Erythropoietin Production in the Kidney and Liver |
| title_fullStr | Effects of Angiotensin II on Erythropoietin Production in the Kidney and Liver |
| title_full_unstemmed | Effects of Angiotensin II on Erythropoietin Production in the Kidney and Liver |
| title_short | Effects of Angiotensin II on Erythropoietin Production in the Kidney and Liver |
| title_sort | effects of angiotensin ii on erythropoietin production in the kidney and liver |
| topic | erythropoietin angiotensin II renin–angiotensin–aldosterone system deglycosylation Western blotting HIF2α |
| url | https://www.mdpi.com/1420-3049/26/17/5399 |
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