Comparison of tests for the detection of circulating filarial antigen (Og4C3-ELISA and AD12-ICT) and ultrasound in diagnosis of lymphatic filariasis in individuals with microfilariae
Significant advances were made in the diagnosis of filariasis in the 1990s with the emergence of three new alternative tools: ultrasound and tests to detect circulating antigen using two monoclonal antibodies, Og4C3 and AD12-ICT-card. This study aimed to identify which of these methods is the most s...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2009-07-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762009000400015 |
| _version_ | 1797756973465206784 |
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| author | Abraham Rocha Cynthia Braga Marcela Belém Arturo Carrera Ana Aguiar-Santos Paula Oliveira Maria José Texeira André Furtado |
| author_facet | Abraham Rocha Cynthia Braga Marcela Belém Arturo Carrera Ana Aguiar-Santos Paula Oliveira Maria José Texeira André Furtado |
| author_sort | Abraham Rocha |
| collection | DOAJ |
| description | Significant advances were made in the diagnosis of filariasis in the 1990s with the emergence of three new alternative tools: ultrasound and tests to detect circulating antigen using two monoclonal antibodies, Og4C3 and AD12-ICT-card. This study aimed to identify which of these methods is the most sensitive for diagnosis of infection. A total of 256 individuals, all male and carrying microfilariae (1-15,679 MF/mL), diagnosed by nocturnal venous blood samples, were tested by all three techniques. The tests for circulating filarial antigen concurred 100% and correctly identified 246/256 (96.69%) of the positive individuals, while ultrasound detected only 186/256 (73.44%). Of the circulating antigen tests, ICT-card was the most convenient method for identification of Wuchereria bancrofti carriers. It was easy to perform, practical and quick. |
| first_indexed | 2024-03-12T18:09:18Z |
| format | Article |
| id | doaj.art-53b51cbe6099401b823ae63a41262d8a |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T18:09:18Z |
| publishDate | 2009-07-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-53b51cbe6099401b823ae63a41262d8a2023-08-02T09:16:34ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80602009-07-01104462162510.1590/S0074-02762009000400015Comparison of tests for the detection of circulating filarial antigen (Og4C3-ELISA and AD12-ICT) and ultrasound in diagnosis of lymphatic filariasis in individuals with microfilariaeAbraham RochaCynthia BragaMarcela BelémArturo CarreraAna Aguiar-SantosPaula OliveiraMaria José TexeiraAndré FurtadoSignificant advances were made in the diagnosis of filariasis in the 1990s with the emergence of three new alternative tools: ultrasound and tests to detect circulating antigen using two monoclonal antibodies, Og4C3 and AD12-ICT-card. This study aimed to identify which of these methods is the most sensitive for diagnosis of infection. A total of 256 individuals, all male and carrying microfilariae (1-15,679 MF/mL), diagnosed by nocturnal venous blood samples, were tested by all three techniques. The tests for circulating filarial antigen concurred 100% and correctly identified 246/256 (96.69%) of the positive individuals, while ultrasound detected only 186/256 (73.44%). Of the circulating antigen tests, ICT-card was the most convenient method for identification of Wuchereria bancrofti carriers. It was easy to perform, practical and quick.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762009000400015Wuchereria bancrofticirculating filarial antigenultrasounddiagnosis |
| spellingShingle | Abraham Rocha Cynthia Braga Marcela Belém Arturo Carrera Ana Aguiar-Santos Paula Oliveira Maria José Texeira André Furtado Comparison of tests for the detection of circulating filarial antigen (Og4C3-ELISA and AD12-ICT) and ultrasound in diagnosis of lymphatic filariasis in individuals with microfilariae Memorias do Instituto Oswaldo Cruz Wuchereria bancrofti circulating filarial antigen ultrasound diagnosis |
| title | Comparison of tests for the detection of circulating filarial antigen (Og4C3-ELISA and AD12-ICT) and ultrasound in diagnosis of lymphatic filariasis in individuals with microfilariae |
| title_full | Comparison of tests for the detection of circulating filarial antigen (Og4C3-ELISA and AD12-ICT) and ultrasound in diagnosis of lymphatic filariasis in individuals with microfilariae |
| title_fullStr | Comparison of tests for the detection of circulating filarial antigen (Og4C3-ELISA and AD12-ICT) and ultrasound in diagnosis of lymphatic filariasis in individuals with microfilariae |
| title_full_unstemmed | Comparison of tests for the detection of circulating filarial antigen (Og4C3-ELISA and AD12-ICT) and ultrasound in diagnosis of lymphatic filariasis in individuals with microfilariae |
| title_short | Comparison of tests for the detection of circulating filarial antigen (Og4C3-ELISA and AD12-ICT) and ultrasound in diagnosis of lymphatic filariasis in individuals with microfilariae |
| title_sort | comparison of tests for the detection of circulating filarial antigen og4c3 elisa and ad12 ict and ultrasound in diagnosis of lymphatic filariasis in individuals with microfilariae |
| topic | Wuchereria bancrofti circulating filarial antigen ultrasound diagnosis |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762009000400015 |
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