<em>Gemmata algarum</em>, a Novel Planctomycete Isolated from an Algal Mat, Displays Antimicrobial Activity
Axenic cultures of two strains, JC673<sup>T</sup> and JC717, both belonging to the phylum <i>Planctomycetota</i>, were isolated from distinct geographical locations in India. Strain JC673<sup>T</sup> was obtained from algal mats of a wetland situated in the state...
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| Format: | Article |
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MDPI AG
2023-12-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/22/1/10 |
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| author | Gaurav Kumar Nicolai Kallscheuer Mohammad Kashif Shabbir Ahamad Uppada Jagadeeshwari Sreya Pannikurungottu Tom Haufschild Moses Kabuu Chintalapati Sasikala Christian Jogler Chintalapati Venkata Ramana |
| author_facet | Gaurav Kumar Nicolai Kallscheuer Mohammad Kashif Shabbir Ahamad Uppada Jagadeeshwari Sreya Pannikurungottu Tom Haufschild Moses Kabuu Chintalapati Sasikala Christian Jogler Chintalapati Venkata Ramana |
| author_sort | Gaurav Kumar |
| collection | DOAJ |
| description | Axenic cultures of two strains, JC673<sup>T</sup> and JC717, both belonging to the phylum <i>Planctomycetota</i>, were isolated from distinct geographical locations in India. Strain JC673<sup>T</sup> was obtained from algal mats of a wetland situated in the state of Kerala, India, while strain JC717 originated from the Central Marine Fisheries Research Institute (CMFRI), state of Tamil Nadu, India. The two strains share 99.9% 16S rRNA gene sequence similarity and are most closely related to <i>Gemmata obscuriglobus</i> UQM 2246<sup>T</sup> (99.3% 16S rRNA gene sequence identity). The newly isolated strains are Gram-negative, grow aerobically and tolerate up to 4% (<i>w</i>/<i>v</i>) NaCl and a pH of up to 9.0. Cells are spherical and form pink-pigmented colonies. The respiratory quinone is MK-6. Major fatty acids are C<sub>18:0</sub>, C<sub>16:1</sub>ω5c and C<sub>16:0</sub>. Polar lipids include phosphatidylcholine, phosphatidylethanolamine, several unidentified amino lipids, unidentified phospholipids, additional unidentified lipids, and an unidentified choline lipid. The polyamine spermidine is produced by the two strains. The strains have a genome size of about 8.2 Mb with a DNA G+C content of 67.6%. Solvent-based culture extracts of the isolates showed antimicrobial activity against three bacterial test strains. Their phylogenetic position along with differences in morphological, physiological, and genomic features support the classification as a new species of the genus <i>Gemmata</i>, for which we propose the name <i>Gemmata algarum</i> sp. nov. Strain JC673<sup>T</sup> (=KCTC 72851<sup>T</sup> = NBRC 114340<sup>T</sup>) and JC717 are the type and non-type strain of the new species, respectively. |
| first_indexed | 2024-03-08T10:43:11Z |
| format | Article |
| id | doaj.art-53b525c8bd0c42f49f5767fb59df731b |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-08T10:43:11Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-53b525c8bd0c42f49f5767fb59df731b2024-01-26T17:25:48ZengMDPI AGMarine Drugs1660-33972023-12-012211010.3390/md22010010<em>Gemmata algarum</em>, a Novel Planctomycete Isolated from an Algal Mat, Displays Antimicrobial ActivityGaurav Kumar0Nicolai Kallscheuer1Mohammad Kashif2Shabbir Ahamad3Uppada Jagadeeshwari4Sreya Pannikurungottu5Tom Haufschild6Moses Kabuu7Chintalapati Sasikala8Christian Jogler9Chintalapati Venkata Ramana10Department of Plant Sciences, School of Life Sciences, University of Hyderabad, P.O. Central University, Hyderabad 500046, IndiaDepartment of Microbial Interactions, Institute of Microbiology, Friedrich Schiller University, 07743 Jena, GermanyDepartment of Plant Sciences, School of Life Sciences, University of Hyderabad, P.O. Central University, Hyderabad 500046, IndiaDepartment of Plant Sciences, School of Life Sciences, University of Hyderabad, P.O. Central University, Hyderabad 500046, IndiaBacterial Discovery Laboratory, Centre for Environment, Institute of Science and Technology, JNT University Hyderabad, Kukatpally, Hyderabad 500085, IndiaDepartment of Plant Sciences, School of Life Sciences, University of Hyderabad, P.O. Central University, Hyderabad 500046, IndiaDepartment of Microbial Interactions, Institute of Microbiology, Friedrich Schiller University, 07743 Jena, GermanyDepartment of Microbial Interactions, Institute of Microbiology, Friedrich Schiller University, 07743 Jena, GermanyBacterial Discovery Laboratory, Centre for Environment, Institute of Science and Technology, JNT University Hyderabad, Kukatpally, Hyderabad 500085, IndiaDepartment of Microbial Interactions, Institute of Microbiology, Friedrich Schiller University, 07743 Jena, GermanyDepartment of Plant Sciences, School of Life Sciences, University of Hyderabad, P.O. Central University, Hyderabad 500046, IndiaAxenic cultures of two strains, JC673<sup>T</sup> and JC717, both belonging to the phylum <i>Planctomycetota</i>, were isolated from distinct geographical locations in India. Strain JC673<sup>T</sup> was obtained from algal mats of a wetland situated in the state of Kerala, India, while strain JC717 originated from the Central Marine Fisheries Research Institute (CMFRI), state of Tamil Nadu, India. The two strains share 99.9% 16S rRNA gene sequence similarity and are most closely related to <i>Gemmata obscuriglobus</i> UQM 2246<sup>T</sup> (99.3% 16S rRNA gene sequence identity). The newly isolated strains are Gram-negative, grow aerobically and tolerate up to 4% (<i>w</i>/<i>v</i>) NaCl and a pH of up to 9.0. Cells are spherical and form pink-pigmented colonies. The respiratory quinone is MK-6. Major fatty acids are C<sub>18:0</sub>, C<sub>16:1</sub>ω5c and C<sub>16:0</sub>. Polar lipids include phosphatidylcholine, phosphatidylethanolamine, several unidentified amino lipids, unidentified phospholipids, additional unidentified lipids, and an unidentified choline lipid. The polyamine spermidine is produced by the two strains. The strains have a genome size of about 8.2 Mb with a DNA G+C content of 67.6%. Solvent-based culture extracts of the isolates showed antimicrobial activity against three bacterial test strains. Their phylogenetic position along with differences in morphological, physiological, and genomic features support the classification as a new species of the genus <i>Gemmata</i>, for which we propose the name <i>Gemmata algarum</i> sp. nov. Strain JC673<sup>T</sup> (=KCTC 72851<sup>T</sup> = NBRC 114340<sup>T</sup>) and JC717 are the type and non-type strain of the new species, respectively.https://www.mdpi.com/1660-3397/22/1/10<i>Gemmataceae</i><i>Planctomycetota</i>bioactivitybuddingwetland bacteriaagar plate assay |
| spellingShingle | Gaurav Kumar Nicolai Kallscheuer Mohammad Kashif Shabbir Ahamad Uppada Jagadeeshwari Sreya Pannikurungottu Tom Haufschild Moses Kabuu Chintalapati Sasikala Christian Jogler Chintalapati Venkata Ramana <em>Gemmata algarum</em>, a Novel Planctomycete Isolated from an Algal Mat, Displays Antimicrobial Activity Marine Drugs <i>Gemmataceae</i> <i>Planctomycetota</i> bioactivity budding wetland bacteria agar plate assay |
| title | <em>Gemmata algarum</em>, a Novel Planctomycete Isolated from an Algal Mat, Displays Antimicrobial Activity |
| title_full | <em>Gemmata algarum</em>, a Novel Planctomycete Isolated from an Algal Mat, Displays Antimicrobial Activity |
| title_fullStr | <em>Gemmata algarum</em>, a Novel Planctomycete Isolated from an Algal Mat, Displays Antimicrobial Activity |
| title_full_unstemmed | <em>Gemmata algarum</em>, a Novel Planctomycete Isolated from an Algal Mat, Displays Antimicrobial Activity |
| title_short | <em>Gemmata algarum</em>, a Novel Planctomycete Isolated from an Algal Mat, Displays Antimicrobial Activity |
| title_sort | em gemmata algarum em a novel planctomycete isolated from an algal mat displays antimicrobial activity |
| topic | <i>Gemmataceae</i> <i>Planctomycetota</i> bioactivity budding wetland bacteria agar plate assay |
| url | https://www.mdpi.com/1660-3397/22/1/10 |
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