ATG3 is subjected to redox regulation to quarantee ATG8 lipidation under ROS-generating stresses
ABSTRACTThe conjugation of ATG8 (autophagy-related 8) proteins to the lipid phosphatidylethanolamine (PE) is the result of the coordinated and highly regulated action of several ATG core proteins, including ATG4 proteases and the E1 (ATG7)- and E2 (ATG3)- activating enzymes. Although it has been sta...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | Autophagy Reports |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/27694127.2023.2300622 |
| _version_ | 1797361207412260864 |
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| author | Manuel J. Mallén-Ponce María Esther Pérez-Pérez |
| author_facet | Manuel J. Mallén-Ponce María Esther Pérez-Pérez |
| author_sort | Manuel J. Mallén-Ponce |
| collection | DOAJ |
| description | ABSTRACTThe conjugation of ATG8 (autophagy-related 8) proteins to the lipid phosphatidylethanolamine (PE) is the result of the coordinated and highly regulated action of several ATG core proteins, including ATG4 proteases and the E1 (ATG7)- and E2 (ATG3)- activating enzymes. Although it has been stablished that ROS signaling plays an important role in autophagy activation, the molecular mechanisms underlying the redox control of ATG proteins remain largely unclear. We have recently shown that ATG3 activity in Chlamydomonas reinhardtii is subjected to reversible redox regulation to ensure ATG8 lipidation and autophagy progression under ROS-linked stress conditions.Abbreviations: ATG, Autophagy-related; Cys, Cysteine; DTTred, Reduced Dithiothreitol; MM(PEG24), Methyl Polyethylene Glycol Maleimide; NEM: N-ethylmaleimide; PE, Phosphatidylethanolamine; ROS, Reactive Oxygen Species; TOR, Target Of Rapamycin; Trx, Thioredoxin; WT, Wild Type |
| first_indexed | 2024-03-08T15:50:37Z |
| format | Article |
| id | doaj.art-53b6ce16bfa240ab8b36b8a6743f2b50 |
| institution | Directory Open Access Journal |
| issn | 2769-4127 |
| language | English |
| last_indexed | 2024-03-08T15:50:37Z |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Autophagy Reports |
| spelling | doaj.art-53b6ce16bfa240ab8b36b8a6743f2b502024-01-09T05:42:48ZengTaylor & Francis GroupAutophagy Reports2769-41272024-12-013110.1080/27694127.2023.2300622ATG3 is subjected to redox regulation to quarantee ATG8 lipidation under ROS-generating stressesManuel J. Mallén-Ponce0María Esther Pérez-Pérez1Instituto de Bioquímica Vegetal y Fotosíntesis, Consejo Superior de Investigaciones Científicas (CSIC)-Universidad de Sevilla Avda. Américo Vespucio 49, 41092 Sevilla, SpainInstituto de Bioquímica Vegetal y Fotosíntesis, Consejo Superior de Investigaciones Científicas (CSIC)-Universidad de Sevilla Avda. Américo Vespucio 49, 41092 Sevilla, SpainABSTRACTThe conjugation of ATG8 (autophagy-related 8) proteins to the lipid phosphatidylethanolamine (PE) is the result of the coordinated and highly regulated action of several ATG core proteins, including ATG4 proteases and the E1 (ATG7)- and E2 (ATG3)- activating enzymes. Although it has been stablished that ROS signaling plays an important role in autophagy activation, the molecular mechanisms underlying the redox control of ATG proteins remain largely unclear. We have recently shown that ATG3 activity in Chlamydomonas reinhardtii is subjected to reversible redox regulation to ensure ATG8 lipidation and autophagy progression under ROS-linked stress conditions.Abbreviations: ATG, Autophagy-related; Cys, Cysteine; DTTred, Reduced Dithiothreitol; MM(PEG24), Methyl Polyethylene Glycol Maleimide; NEM: N-ethylmaleimide; PE, Phosphatidylethanolamine; ROS, Reactive Oxygen Species; TOR, Target Of Rapamycin; Trx, Thioredoxin; WT, Wild Typehttps://www.tandfonline.com/doi/10.1080/27694127.2023.2300622ATG3ATG8 lipidationChlamydomonasROSredoxstress |
| spellingShingle | Manuel J. Mallén-Ponce María Esther Pérez-Pérez ATG3 is subjected to redox regulation to quarantee ATG8 lipidation under ROS-generating stresses Autophagy Reports ATG3 ATG8 lipidation Chlamydomonas ROS redox stress |
| title | ATG3 is subjected to redox regulation to quarantee ATG8 lipidation under ROS-generating stresses |
| title_full | ATG3 is subjected to redox regulation to quarantee ATG8 lipidation under ROS-generating stresses |
| title_fullStr | ATG3 is subjected to redox regulation to quarantee ATG8 lipidation under ROS-generating stresses |
| title_full_unstemmed | ATG3 is subjected to redox regulation to quarantee ATG8 lipidation under ROS-generating stresses |
| title_short | ATG3 is subjected to redox regulation to quarantee ATG8 lipidation under ROS-generating stresses |
| title_sort | atg3 is subjected to redox regulation to quarantee atg8 lipidation under ros generating stresses |
| topic | ATG3 ATG8 lipidation Chlamydomonas ROS redox stress |
| url | https://www.tandfonline.com/doi/10.1080/27694127.2023.2300622 |
| work_keys_str_mv | AT manueljmallenponce atg3issubjectedtoredoxregulationtoquaranteeatg8lipidationunderrosgeneratingstresses AT mariaestherperezperez atg3issubjectedtoredoxregulationtoquaranteeatg8lipidationunderrosgeneratingstresses |