Integrating Molecular Network and Culture Media Variation to Explore the Production of Bioactive Metabolites by <i>Vibrio diabolicus</i> A1SM3
<i>Vibrio diabolicus</i> A1SM3 strain was isolated from a sediment sample from Manaure Solar Saltern in La Guajira and the produced crude extracts have shown antibacterial activity against methicillin-resistant <i>Staphylococcus aureus</i> and cytotoxic activity against human...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-03-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/17/4/196 |
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| author | Natalia Conde-Martínez Anelize Bauermeister Alan Cesar Pilon Norberto Peporine Lopes Edisson Tello |
| author_facet | Natalia Conde-Martínez Anelize Bauermeister Alan Cesar Pilon Norberto Peporine Lopes Edisson Tello |
| author_sort | Natalia Conde-Martínez |
| collection | DOAJ |
| description | <i>Vibrio diabolicus</i> A1SM3 strain was isolated from a sediment sample from Manaure Solar Saltern in La Guajira and the produced crude extracts have shown antibacterial activity against methicillin-resistant <i>Staphylococcus aureus</i> and cytotoxic activity against human lung cell line. Thus, the aim of this research was to identify the main compound responsible for the biological activity observed and to systematically study how each carbon and nitrogen source in the growth media, and variation of the salinity, affect its production. For the characterization of the bioactive metabolites, 15 fractions obtained from <i>Vibrio diabolicus</i> A1SM3 crude extract were analyzed by HPLC-MS/MS and their activity was established. The bioactive fractions were dereplicated with Antibase and Marinlit databases, which combined with nuclear magnetic resonance (NMR) spectra and fragmentation by MS/MS, led to the identification of 2,2-di(3-indolyl)-3-indolone (isotrisindoline), an indole-derivative antibiotic, previously isolated from marine organisms. The influence of the variations of the culture media in isotrisindoline production was established by molecular network and MZmine showing that the media containing starch and peptone at 7% NaCl was the best culture media to produce it. Also, polyhydroxybutyrates (PHB) identification was established by MS/MS mainly in casamino acids media, contributing to the first report on PHB production by this strain. |
| first_indexed | 2024-12-10T07:15:35Z |
| format | Article |
| id | doaj.art-53b9138de2f04a888dfb54361c12fdd5 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-12-10T07:15:35Z |
| publishDate | 2019-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-53b9138de2f04a888dfb54361c12fdd52022-12-22T01:57:57ZengMDPI AGMarine Drugs1660-33972019-03-0117419610.3390/md17040196md17040196Integrating Molecular Network and Culture Media Variation to Explore the Production of Bioactive Metabolites by <i>Vibrio diabolicus</i> A1SM3Natalia Conde-Martínez0Anelize Bauermeister1Alan Cesar Pilon2Norberto Peporine Lopes3Edisson Tello4Bioscience Doctoral Program, Grupo de Investigación en Bioprospección, Faculty of Engineering, Universidad de La Sabana, Campus Puente del Común, Km 7, Autopista Norte de Bogotá, 250001 Chía, ColombiaNúcleo de Pesquisa em Produtos Naturais e Sintéticos (NPPNS), Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, 14040-903 Ribeirão Preto/SP, BrazilNúcleo de Pesquisa em Produtos Naturais e Sintéticos (NPPNS), Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, 14040-903 Ribeirão Preto/SP, BrazilNúcleo de Pesquisa em Produtos Naturais e Sintéticos (NPPNS), Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, 14040-903 Ribeirão Preto/SP, BrazilBioscience Doctoral Program, Grupo de Investigación en Bioprospección, Faculty of Engineering, Universidad de La Sabana, Campus Puente del Común, Km 7, Autopista Norte de Bogotá, 250001 Chía, Colombia<i>Vibrio diabolicus</i> A1SM3 strain was isolated from a sediment sample from Manaure Solar Saltern in La Guajira and the produced crude extracts have shown antibacterial activity against methicillin-resistant <i>Staphylococcus aureus</i> and cytotoxic activity against human lung cell line. Thus, the aim of this research was to identify the main compound responsible for the biological activity observed and to systematically study how each carbon and nitrogen source in the growth media, and variation of the salinity, affect its production. For the characterization of the bioactive metabolites, 15 fractions obtained from <i>Vibrio diabolicus</i> A1SM3 crude extract were analyzed by HPLC-MS/MS and their activity was established. The bioactive fractions were dereplicated with Antibase and Marinlit databases, which combined with nuclear magnetic resonance (NMR) spectra and fragmentation by MS/MS, led to the identification of 2,2-di(3-indolyl)-3-indolone (isotrisindoline), an indole-derivative antibiotic, previously isolated from marine organisms. The influence of the variations of the culture media in isotrisindoline production was established by molecular network and MZmine showing that the media containing starch and peptone at 7% NaCl was the best culture media to produce it. Also, polyhydroxybutyrates (PHB) identification was established by MS/MS mainly in casamino acids media, contributing to the first report on PHB production by this strain.https://www.mdpi.com/1660-3397/17/4/196<i>Vibrio diabolicus</i>molecular networking2,2-di(3-indolyl)-3-indolonePHBMS/MSsolar saltern |
| spellingShingle | Natalia Conde-Martínez Anelize Bauermeister Alan Cesar Pilon Norberto Peporine Lopes Edisson Tello Integrating Molecular Network and Culture Media Variation to Explore the Production of Bioactive Metabolites by <i>Vibrio diabolicus</i> A1SM3 Marine Drugs <i>Vibrio diabolicus</i> molecular networking 2,2-di(3-indolyl)-3-indolone PHB MS/MS solar saltern |
| title | Integrating Molecular Network and Culture Media Variation to Explore the Production of Bioactive Metabolites by <i>Vibrio diabolicus</i> A1SM3 |
| title_full | Integrating Molecular Network and Culture Media Variation to Explore the Production of Bioactive Metabolites by <i>Vibrio diabolicus</i> A1SM3 |
| title_fullStr | Integrating Molecular Network and Culture Media Variation to Explore the Production of Bioactive Metabolites by <i>Vibrio diabolicus</i> A1SM3 |
| title_full_unstemmed | Integrating Molecular Network and Culture Media Variation to Explore the Production of Bioactive Metabolites by <i>Vibrio diabolicus</i> A1SM3 |
| title_short | Integrating Molecular Network and Culture Media Variation to Explore the Production of Bioactive Metabolites by <i>Vibrio diabolicus</i> A1SM3 |
| title_sort | integrating molecular network and culture media variation to explore the production of bioactive metabolites by i vibrio diabolicus i a1sm3 |
| topic | <i>Vibrio diabolicus</i> molecular networking 2,2-di(3-indolyl)-3-indolone PHB MS/MS solar saltern |
| url | https://www.mdpi.com/1660-3397/17/4/196 |
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