Acceleration of burn wound healing by micronized amniotic membrane seeded with umbilical cord-derived mesenchymal stem cells
Umbilical cord-derived mesenchymal stem cells (UC-MSC) are promising candidates for wound healing. However, the low amplification efficiency of MSC in vitro and their low survival rates after transplantation have limited their medical application. In this study, we fabricated a micronized amniotic m...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-06-01
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| Series: | Materials Today Bio |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590006423001461 |
| _version_ | 1797796387024273408 |
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| author | Zixuan Zhou Jingnan Xun Chenghao Wu Chao Ji Shizhao Ji Futing Shu Yuxiang Wang Hao Chen Yongjun Zheng Shichu Xiao |
| author_facet | Zixuan Zhou Jingnan Xun Chenghao Wu Chao Ji Shizhao Ji Futing Shu Yuxiang Wang Hao Chen Yongjun Zheng Shichu Xiao |
| author_sort | Zixuan Zhou |
| collection | DOAJ |
| description | Umbilical cord-derived mesenchymal stem cells (UC-MSC) are promising candidates for wound healing. However, the low amplification efficiency of MSC in vitro and their low survival rates after transplantation have limited their medical application. In this study, we fabricated a micronized amniotic membrane (mAM) as a microcarrier to amplify MSC in vitro and used mAM and MSC (mAM-MSC) complexes to repair burn wounds. Results showed that MSC could live and proliferate on mAM in a 3D culture system, exhibiting higher cell activity than in 2D culture. Transcriptome sequencing of MSC showed that the expression of growth factor-related, angiogenesis-related, and wound healing-related genes was significantly upregulated in mAM-MSC compared to traditional 2D-cultured MSC, which was verified via RT-qPCR. Gene ontology (GO) analysis of differentially expressed genes (DEGs) showed significant enrichment of terms related to cell proliferation, angiogenesis, cytokine activity, and wound healing in mAM-MSC. In a burn wound model of C57BL/6J mice, topical application of mAM-MSC significantly accelerated wound healing compared to MSC injection alone and was accompanied by longer survival of MSC and greater neovascularization in the wound. |
| first_indexed | 2024-03-13T03:32:19Z |
| format | Article |
| id | doaj.art-53bb20a417a443468384dc77ff36be3f |
| institution | Directory Open Access Journal |
| issn | 2590-0064 |
| language | English |
| last_indexed | 2024-03-13T03:32:19Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials Today Bio |
| spelling | doaj.art-53bb20a417a443468384dc77ff36be3f2023-06-24T05:18:48ZengElsevierMaterials Today Bio2590-00642023-06-0120100686Acceleration of burn wound healing by micronized amniotic membrane seeded with umbilical cord-derived mesenchymal stem cellsZixuan Zhou0Jingnan Xun1Chenghao Wu2Chao Ji3Shizhao Ji4Futing Shu5Yuxiang Wang6Hao Chen7Yongjun Zheng8Shichu Xiao9Department of Burn Surgery, The First Affiliated Hospital of Naval Medical University, Shanghai, 200433, People's Republic of ChinaDepartment of Burn Surgery, The First Affiliated Hospital of Naval Medical University, Shanghai, 200433, People's Republic of ChinaDepartment of Obstetrics and Gynecology, Tongji Hospital, Tongji University School of Medicine, Shanghai, 200065, People's Republic of ChinaDepartment of Burn Surgery, The First Affiliated Hospital of Naval Medical University, Shanghai, 200433, People's Republic of ChinaDepartment of Burn Surgery, The First Affiliated Hospital of Naval Medical University, Shanghai, 200433, People's Republic of ChinaDepartment of Burn Surgery, The First Affiliated Hospital of Naval Medical University, Shanghai, 200433, People's Republic of ChinaDepartment of Burn Surgery, The First Affiliated Hospital of Naval Medical University, Shanghai, 200433, People's Republic of ChinaDepartment of Burn Surgery, The First Affiliated Hospital of Naval Medical University, Shanghai, 200433, People's Republic of ChinaDepartment of Burn Surgery, The First Affiliated Hospital of Naval Medical University, Shanghai, 200433, People's Republic of China; Corresponding author.Department of Burn Surgery, The First Affiliated Hospital of Naval Medical University, Shanghai, 200433, People's Republic of China; Corresponding author.Umbilical cord-derived mesenchymal stem cells (UC-MSC) are promising candidates for wound healing. However, the low amplification efficiency of MSC in vitro and their low survival rates after transplantation have limited their medical application. In this study, we fabricated a micronized amniotic membrane (mAM) as a microcarrier to amplify MSC in vitro and used mAM and MSC (mAM-MSC) complexes to repair burn wounds. Results showed that MSC could live and proliferate on mAM in a 3D culture system, exhibiting higher cell activity than in 2D culture. Transcriptome sequencing of MSC showed that the expression of growth factor-related, angiogenesis-related, and wound healing-related genes was significantly upregulated in mAM-MSC compared to traditional 2D-cultured MSC, which was verified via RT-qPCR. Gene ontology (GO) analysis of differentially expressed genes (DEGs) showed significant enrichment of terms related to cell proliferation, angiogenesis, cytokine activity, and wound healing in mAM-MSC. In a burn wound model of C57BL/6J mice, topical application of mAM-MSC significantly accelerated wound healing compared to MSC injection alone and was accompanied by longer survival of MSC and greater neovascularization in the wound.http://www.sciencedirect.com/science/article/pii/S2590006423001461Burn wound healingMicrocarrierAmniotic membraneUmbilical cord-derived mesenchymal stem cells |
| spellingShingle | Zixuan Zhou Jingnan Xun Chenghao Wu Chao Ji Shizhao Ji Futing Shu Yuxiang Wang Hao Chen Yongjun Zheng Shichu Xiao Acceleration of burn wound healing by micronized amniotic membrane seeded with umbilical cord-derived mesenchymal stem cells Materials Today Bio Burn wound healing Microcarrier Amniotic membrane Umbilical cord-derived mesenchymal stem cells |
| title | Acceleration of burn wound healing by micronized amniotic membrane seeded with umbilical cord-derived mesenchymal stem cells |
| title_full | Acceleration of burn wound healing by micronized amniotic membrane seeded with umbilical cord-derived mesenchymal stem cells |
| title_fullStr | Acceleration of burn wound healing by micronized amniotic membrane seeded with umbilical cord-derived mesenchymal stem cells |
| title_full_unstemmed | Acceleration of burn wound healing by micronized amniotic membrane seeded with umbilical cord-derived mesenchymal stem cells |
| title_short | Acceleration of burn wound healing by micronized amniotic membrane seeded with umbilical cord-derived mesenchymal stem cells |
| title_sort | acceleration of burn wound healing by micronized amniotic membrane seeded with umbilical cord derived mesenchymal stem cells |
| topic | Burn wound healing Microcarrier Amniotic membrane Umbilical cord-derived mesenchymal stem cells |
| url | http://www.sciencedirect.com/science/article/pii/S2590006423001461 |
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