Regulation of eukaryotic mRNA deadenylation and degradation by the Ccr4-Not complex
Accurate and precise regulation of gene expression programmes in eukaryotes involves the coordinated control of transcription, mRNA stability and translation. In recent years, significant progress has been made about the role of sequence elements in the 3′ untranslated region for the regulation of m...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2023-04-01
|
| Series: | Frontiers in Cell and Developmental Biology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fcell.2023.1153624/full |
| _version_ | 1797843569129553920 |
|---|---|
| author | Lorenzo Pavanello Michael Hall Gerlof Sebastiaan Winkler |
| author_facet | Lorenzo Pavanello Michael Hall Gerlof Sebastiaan Winkler |
| author_sort | Lorenzo Pavanello |
| collection | DOAJ |
| description | Accurate and precise regulation of gene expression programmes in eukaryotes involves the coordinated control of transcription, mRNA stability and translation. In recent years, significant progress has been made about the role of sequence elements in the 3′ untranslated region for the regulation of mRNA degradation, and a model has emerged in which recruitment of the Ccr4-Not complex is the critical step in the regulation of mRNA decay. Recruitment of the Ccr4-Not complex to a target mRNA results in deadenylation mediated by the Caf1 and Ccr4 catalytic subunits of the complex. Following deadenylation, the 5′ cap structure is removed, and the mRNA subjected to 5′-3′ degradation. Here, the role of the human Ccr4-Not complex in cytoplasmic deadenylation of mRNA is reviewed, with a particular focus on mechanisms of its recruitment to mRNA by sequence motifs in the 3′ untranslated region, codon usage, as well as general mechanisms involving the poly(A) tail. |
| first_indexed | 2024-04-09T17:08:31Z |
| format | Article |
| id | doaj.art-53be0c7e7d394f4ab0e6819eb79ecc9f |
| institution | Directory Open Access Journal |
| issn | 2296-634X |
| language | English |
| last_indexed | 2024-04-09T17:08:31Z |
| publishDate | 2023-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj.art-53be0c7e7d394f4ab0e6819eb79ecc9f2023-04-20T09:02:30ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2023-04-011110.3389/fcell.2023.11536241153624Regulation of eukaryotic mRNA deadenylation and degradation by the Ccr4-Not complexLorenzo Pavanello0Michael Hall1Gerlof Sebastiaan Winkler2School of Pharmacy, University of Nottingham, University Park, Nottingham, United KingdomSchool of Pharmacy, University of Nottingham, University Park, Nottingham, United KingdomSchool of Pharmacy, University of Nottingham, University Park, Nottingham, United KingdomAccurate and precise regulation of gene expression programmes in eukaryotes involves the coordinated control of transcription, mRNA stability and translation. In recent years, significant progress has been made about the role of sequence elements in the 3′ untranslated region for the regulation of mRNA degradation, and a model has emerged in which recruitment of the Ccr4-Not complex is the critical step in the regulation of mRNA decay. Recruitment of the Ccr4-Not complex to a target mRNA results in deadenylation mediated by the Caf1 and Ccr4 catalytic subunits of the complex. Following deadenylation, the 5′ cap structure is removed, and the mRNA subjected to 5′-3′ degradation. Here, the role of the human Ccr4-Not complex in cytoplasmic deadenylation of mRNA is reviewed, with a particular focus on mechanisms of its recruitment to mRNA by sequence motifs in the 3′ untranslated region, codon usage, as well as general mechanisms involving the poly(A) tail.https://www.frontiersin.org/articles/10.3389/fcell.2023.1153624/fullmRNAdeadenylationdegradationCcr4-Notdeadenylasegene regulation |
| spellingShingle | Lorenzo Pavanello Michael Hall Gerlof Sebastiaan Winkler Regulation of eukaryotic mRNA deadenylation and degradation by the Ccr4-Not complex Frontiers in Cell and Developmental Biology mRNA deadenylation degradation Ccr4-Not deadenylase gene regulation |
| title | Regulation of eukaryotic mRNA deadenylation and degradation by the Ccr4-Not complex |
| title_full | Regulation of eukaryotic mRNA deadenylation and degradation by the Ccr4-Not complex |
| title_fullStr | Regulation of eukaryotic mRNA deadenylation and degradation by the Ccr4-Not complex |
| title_full_unstemmed | Regulation of eukaryotic mRNA deadenylation and degradation by the Ccr4-Not complex |
| title_short | Regulation of eukaryotic mRNA deadenylation and degradation by the Ccr4-Not complex |
| title_sort | regulation of eukaryotic mrna deadenylation and degradation by the ccr4 not complex |
| topic | mRNA deadenylation degradation Ccr4-Not deadenylase gene regulation |
| url | https://www.frontiersin.org/articles/10.3389/fcell.2023.1153624/full |
| work_keys_str_mv | AT lorenzopavanello regulationofeukaryoticmrnadeadenylationanddegradationbytheccr4notcomplex AT michaelhall regulationofeukaryoticmrnadeadenylationanddegradationbytheccr4notcomplex AT gerlofsebastiaanwinkler regulationofeukaryoticmrnadeadenylationanddegradationbytheccr4notcomplex |