Evaluation of Diagnostic Potential of Echinococcus granulosus Recombinant EgAgB8/1, EgAgB8/2 and EPC1 Antigens for Cystic Echinococcosis in Goats
Echinococcus granulosus recombinant proteins including two antigen B sub-units EgAgB8/1 and EgAgB8/2 and Echinococcus protoscolex calcium binding protein (EPC1) were expressed in prokaryotic expression vectors. The diagnostic potential of these three recombinant proteins was evaluated in the detecti...
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Journal of Pure and Applied Microbiology
2017-03-01
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| Series: | Journal of Pure and Applied Microbiology |
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| Online Access: | https://microbiologyjournal.org/evaluation-of-diagnostic-potential-of-echinococcus-granulosus-recombinant-egagb8-1-egagb8-2-and-epc1-antigens-for-cystic-echinococcosis-in-goats/ |
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| author | Ajayta Rialch Mary Nisha Tigga Arun A S. Samanta O.K. Raina |
| author_facet | Ajayta Rialch Mary Nisha Tigga Arun A S. Samanta O.K. Raina |
| author_sort | Ajayta Rialch |
| collection | DOAJ |
| description | Echinococcus granulosus recombinant proteins including two antigen B sub-units EgAgB8/1 and EgAgB8/2 and Echinococcus protoscolex calcium binding protein (EPC1) were expressed in prokaryotic expression vectors. The diagnostic potential of these three recombinant proteins was evaluated in the detection of cystic echinococcosis in goats in IgG-ELISA. The EgAgB8/1 and EgAgB8/2 recombinant proteins reacted fairly with the hydatid infected goats with sensitivity of 66.7% and 80.0% and specificity of 71.3% and 73.3%, respectively while EPC1 recombinant protein showed lower sensitivity (60%) but comparable specificity (72.3%). Cross-reactivity of these three antigens with goat gastro-intestinal strongyle nematodes and Taenia hydatigena under field conditions was studied. Results showed that EgAgB8/1, EgAgB8/2 and EPC1 antigens cross-reacted with most of the parasites in the goat host. |
| first_indexed | 2024-12-20T18:50:52Z |
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| id | doaj.art-53c2f5c322f84fdda0381c16c0a9d1da |
| institution | Directory Open Access Journal |
| issn | 0973-7510 2581-690X |
| language | English |
| last_indexed | 2024-12-20T18:50:52Z |
| publishDate | 2017-03-01 |
| publisher | Journal of Pure and Applied Microbiology |
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| series | Journal of Pure and Applied Microbiology |
| spelling | doaj.art-53c2f5c322f84fdda0381c16c0a9d1da2022-12-21T19:29:36ZengJournal of Pure and Applied MicrobiologyJournal of Pure and Applied Microbiology0973-75102581-690X2017-03-0111144144810.22207/JPAM.11.1.58Evaluation of Diagnostic Potential of Echinococcus granulosus Recombinant EgAgB8/1, EgAgB8/2 and EPC1 Antigens for Cystic Echinococcosis in GoatsAjayta Rialch0Mary Nisha Tigga1Arun A2S. Samanta3O.K. Raina4Division of Parasitology, Indian Veterinary Research Institute, Izatnagar - 243 122, IndiaDivision of Parasitology, Indian Veterinary Research Institute, Izatnagar - 243 122, IndiaDivision of Parasitology, Indian Veterinary Research Institute, Izatnagar - 243 122, IndiaDivision of Parasitology, Indian Veterinary Research Institute, Izatnagar - 243 122, IndiaDivision of Parasitology, Indian Veterinary Research Institute, Izatnagar - 243 122, IndiaEchinococcus granulosus recombinant proteins including two antigen B sub-units EgAgB8/1 and EgAgB8/2 and Echinococcus protoscolex calcium binding protein (EPC1) were expressed in prokaryotic expression vectors. The diagnostic potential of these three recombinant proteins was evaluated in the detection of cystic echinococcosis in goats in IgG-ELISA. The EgAgB8/1 and EgAgB8/2 recombinant proteins reacted fairly with the hydatid infected goats with sensitivity of 66.7% and 80.0% and specificity of 71.3% and 73.3%, respectively while EPC1 recombinant protein showed lower sensitivity (60%) but comparable specificity (72.3%). Cross-reactivity of these three antigens with goat gastro-intestinal strongyle nematodes and Taenia hydatigena under field conditions was studied. Results showed that EgAgB8/1, EgAgB8/2 and EPC1 antigens cross-reacted with most of the parasites in the goat host.https://microbiologyjournal.org/evaluation-of-diagnostic-potential-of-echinococcus-granulosus-recombinant-egagb8-1-egagb8-2-and-epc1-antigens-for-cystic-echinococcosis-in-goats/echinococcus granulosusgoatantigen b8 sub-unitsepc1diagnosisigg-elisa |
| spellingShingle | Ajayta Rialch Mary Nisha Tigga Arun A S. Samanta O.K. Raina Evaluation of Diagnostic Potential of Echinococcus granulosus Recombinant EgAgB8/1, EgAgB8/2 and EPC1 Antigens for Cystic Echinococcosis in Goats Journal of Pure and Applied Microbiology echinococcus granulosus goat antigen b8 sub-units epc1 diagnosis igg-elisa |
| title | Evaluation of Diagnostic Potential of Echinococcus granulosus Recombinant EgAgB8/1, EgAgB8/2 and EPC1 Antigens for Cystic Echinococcosis in Goats |
| title_full | Evaluation of Diagnostic Potential of Echinococcus granulosus Recombinant EgAgB8/1, EgAgB8/2 and EPC1 Antigens for Cystic Echinococcosis in Goats |
| title_fullStr | Evaluation of Diagnostic Potential of Echinococcus granulosus Recombinant EgAgB8/1, EgAgB8/2 and EPC1 Antigens for Cystic Echinococcosis in Goats |
| title_full_unstemmed | Evaluation of Diagnostic Potential of Echinococcus granulosus Recombinant EgAgB8/1, EgAgB8/2 and EPC1 Antigens for Cystic Echinococcosis in Goats |
| title_short | Evaluation of Diagnostic Potential of Echinococcus granulosus Recombinant EgAgB8/1, EgAgB8/2 and EPC1 Antigens for Cystic Echinococcosis in Goats |
| title_sort | evaluation of diagnostic potential of echinococcus granulosus recombinant egagb8 1 egagb8 2 and epc1 antigens for cystic echinococcosis in goats |
| topic | echinococcus granulosus goat antigen b8 sub-units epc1 diagnosis igg-elisa |
| url | https://microbiologyjournal.org/evaluation-of-diagnostic-potential-of-echinococcus-granulosus-recombinant-egagb8-1-egagb8-2-and-epc1-antigens-for-cystic-echinococcosis-in-goats/ |
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