Larynx proteomics after jellyfish collagen IL: Increased ECM/collagen and suppressed inflammation
Abstract Objectives/Hypothesis Compare proteomic profiles of rabbit vocal folds (VFs) injected with micronized cross‐linked jellyfish collagen “collagen Type 0” (MX‐JC) against two clinical products for injection medialization laryngoplasty (IL). Study Design Animal model. Methods Left recurrent lar...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2022-10-01
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| Series: | Laryngoscope Investigative Otolaryngology |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/lio2.924 |
| _version_ | 1811238298239107072 |
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| author | Andrew J. Bowen Dale C. Ekbom Danielle Hunter Stephen Voss Kathleen Bartemes Andrew Mearns‐Spragg Michael S. Oldenburg Serban San‐Marina |
| author_facet | Andrew J. Bowen Dale C. Ekbom Danielle Hunter Stephen Voss Kathleen Bartemes Andrew Mearns‐Spragg Michael S. Oldenburg Serban San‐Marina |
| author_sort | Andrew J. Bowen |
| collection | DOAJ |
| description | Abstract Objectives/Hypothesis Compare proteomic profiles of rabbit vocal folds (VFs) injected with micronized cross‐linked jellyfish collagen “collagen Type 0” (MX‐JC) against two clinical products for injection medialization laryngoplasty (IL). Study Design Animal model. Methods Left recurrent laryngeal nerve sectioning and IL were performed in New Zealand White rabbits (N = 6/group). Group 1 received (MX‐JC) and adipose‐derived stem cells (ADSCs), Group 2, MX‐JC alone; Group 3, cross‐linked hyaluronic acid; and Group 4, micronized acellular dermis. Animals were sacrificed at 4 and 12 weeks. Proteomic profiling of injected versus noninjected VFs by nano‐liquid chromatography, tandem mass spectrometry, and reactome gene ontology analysis was performed. Results Overall, 37–61 proteins were found to be upregulated and 60–284 downregulated in injected versus non‐injected VFs (>1.5 fold, false discovery rate‐adjusted p < .05). Over‐representation analysis (% of total) revealed top up‐regulated pathways at 4 and 12 weeks, respectively: Group 1, keratan sulfate metabolism (46%) and cellular processes (29%); Group 2, extracellular matrix (ECM)/collagen processes (33%) and beta oxidation (39%); Group 3, cellular processes (50%) and energy metabolism (100%); and Group 4, keratan sulfate metabolism (31%) and inflammation (50%). Top downregulated pathways were: Group 1, Inflammation (36%) and glucose/citric acid metabolism (42%); Group 2, cell signaling (38%) and glucose/citric acid metabolism (35%); Group 3, keratan sulfate metabolism (31%) and ECM/collagen processes (48%); and Group 4, glucose/citric acid metabolism (33%) and ECM/collagen processes (43%). Conclusions MX‐JC “collagen Type 0” upregulates pathways related to ECM/collagen formation and downregulates pathways related to inflammation suggesting that it is promising biomaterial for IL. Level of Evidence NA |
| first_indexed | 2024-04-12T12:39:19Z |
| format | Article |
| id | doaj.art-53c642b82d91436e829d199090111ee4 |
| institution | Directory Open Access Journal |
| issn | 2378-8038 |
| language | English |
| last_indexed | 2024-04-12T12:39:19Z |
| publishDate | 2022-10-01 |
| publisher | Wiley |
| record_format | Article |
| series | Laryngoscope Investigative Otolaryngology |
| spelling | doaj.art-53c642b82d91436e829d199090111ee42022-12-22T03:32:49ZengWileyLaryngoscope Investigative Otolaryngology2378-80382022-10-01751513152010.1002/lio2.924Larynx proteomics after jellyfish collagen IL: Increased ECM/collagen and suppressed inflammationAndrew J. Bowen0Dale C. Ekbom1Danielle Hunter2Stephen Voss3Kathleen Bartemes4Andrew Mearns‐Spragg5Michael S. Oldenburg6Serban San‐Marina7Mayo Clinic ENT Rochester Minnesota USAMayo Clinic ENT Rochester Minnesota USAMayo Clinic ENT Rochester Minnesota USAMayo Clinic ENT Rochester Minnesota USAMayo Clinic ENT Rochester Minnesota USAJellagen Ltd. Wentloog Industrial Estate Cardiff UKPrevea Health Services Green Bay Wisconsin USAMayo Clinic ENT Rochester Minnesota USAAbstract Objectives/Hypothesis Compare proteomic profiles of rabbit vocal folds (VFs) injected with micronized cross‐linked jellyfish collagen “collagen Type 0” (MX‐JC) against two clinical products for injection medialization laryngoplasty (IL). Study Design Animal model. Methods Left recurrent laryngeal nerve sectioning and IL were performed in New Zealand White rabbits (N = 6/group). Group 1 received (MX‐JC) and adipose‐derived stem cells (ADSCs), Group 2, MX‐JC alone; Group 3, cross‐linked hyaluronic acid; and Group 4, micronized acellular dermis. Animals were sacrificed at 4 and 12 weeks. Proteomic profiling of injected versus noninjected VFs by nano‐liquid chromatography, tandem mass spectrometry, and reactome gene ontology analysis was performed. Results Overall, 37–61 proteins were found to be upregulated and 60–284 downregulated in injected versus non‐injected VFs (>1.5 fold, false discovery rate‐adjusted p < .05). Over‐representation analysis (% of total) revealed top up‐regulated pathways at 4 and 12 weeks, respectively: Group 1, keratan sulfate metabolism (46%) and cellular processes (29%); Group 2, extracellular matrix (ECM)/collagen processes (33%) and beta oxidation (39%); Group 3, cellular processes (50%) and energy metabolism (100%); and Group 4, keratan sulfate metabolism (31%) and inflammation (50%). Top downregulated pathways were: Group 1, Inflammation (36%) and glucose/citric acid metabolism (42%); Group 2, cell signaling (38%) and glucose/citric acid metabolism (35%); Group 3, keratan sulfate metabolism (31%) and ECM/collagen processes (48%); and Group 4, glucose/citric acid metabolism (33%) and ECM/collagen processes (43%). Conclusions MX‐JC “collagen Type 0” upregulates pathways related to ECM/collagen formation and downregulates pathways related to inflammation suggesting that it is promising biomaterial for IL. Level of Evidence NAhttps://doi.org/10.1002/lio2.924collagen type 0Cymetra®hyaluronic acidinjection laryngoplastyJellagen®jellyfish collagen |
| spellingShingle | Andrew J. Bowen Dale C. Ekbom Danielle Hunter Stephen Voss Kathleen Bartemes Andrew Mearns‐Spragg Michael S. Oldenburg Serban San‐Marina Larynx proteomics after jellyfish collagen IL: Increased ECM/collagen and suppressed inflammation Laryngoscope Investigative Otolaryngology collagen type 0 Cymetra® hyaluronic acid injection laryngoplasty Jellagen® jellyfish collagen |
| title | Larynx proteomics after jellyfish collagen IL: Increased ECM/collagen and suppressed inflammation |
| title_full | Larynx proteomics after jellyfish collagen IL: Increased ECM/collagen and suppressed inflammation |
| title_fullStr | Larynx proteomics after jellyfish collagen IL: Increased ECM/collagen and suppressed inflammation |
| title_full_unstemmed | Larynx proteomics after jellyfish collagen IL: Increased ECM/collagen and suppressed inflammation |
| title_short | Larynx proteomics after jellyfish collagen IL: Increased ECM/collagen and suppressed inflammation |
| title_sort | larynx proteomics after jellyfish collagen il increased ecm collagen and suppressed inflammation |
| topic | collagen type 0 Cymetra® hyaluronic acid injection laryngoplasty Jellagen® jellyfish collagen |
| url | https://doi.org/10.1002/lio2.924 |
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