Protein Extract of Tobacco Expressing <i>Solanum torvum</i> PP5-Encoding Gene Inhibits <i>Verticillium dahliae</i> Proliferation
Verticillium wilt, a soilborne disease caused by <i>Verticillium dahliae</i> (<i>V. dahliae</i>), can severely affect the yields of Solanaceae crops. In a previous study, it was observed in <i>Solanum torvum</i> (<i>S. torvum</i>) that protein phosphat...
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MDPI AG
2022-03-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/8/3/252 |
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| author | Min Chen Chao Xie Qing Yang Wenjiao Zhu Man Wang |
| author_facet | Min Chen Chao Xie Qing Yang Wenjiao Zhu Man Wang |
| author_sort | Min Chen |
| collection | DOAJ |
| description | Verticillium wilt, a soilborne disease caused by <i>Verticillium dahliae</i> (<i>V. dahliae</i>), can severely affect the yields of Solanaceae crops. In a previous study, it was observed in <i>Solanum torvum</i> (<i>S. torvum</i>) that protein phosphatase 5 (PP5) was induced by <i>V. dahliae</i> infection. To elucidate the function of PP5 more clearly, this study cloned an <i>StPP5</i> cDNA from <i>S. torvum</i> by PCR. The cDNA contained an ORF of 1458 bp long encoding a putative protein of 485 amino acid residues with a predicted molecular mass of 54.63 kDa and a theoretical isoelectric point of 5.66. StPP5 protein contained a conserved PP domain and showed high similarity to other homologous members of the PP5 family from various plant species. The expression of <i>StPP5</i> gene was upregulated after <i>V.</i> infection and reached its maximum value at 24 h in leaves. In order to clarify the role of <i>StPP5</i>, four transgenic tobacco plants expressing <i>StPP5</i> were generated through <i>Agrobacterium</i>-mediated transformation and identified by PCR. In vitro culture assay showed that the growth of <i>V. dahliae</i> in PDA medium containing proteins extracted from the leaves of transgenic tobacco line P6 was inhibited, whose inhibition rate was 55.1%, higher than the non-transgenic control. These results indicated that StPP5 might be involved in plant defense against <i>V. dahliae</i> infection. |
| first_indexed | 2024-03-09T19:45:18Z |
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| last_indexed | 2024-03-09T19:45:18Z |
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| spelling | doaj.art-53c8f65b50e348fead1469ff1c97d23e2023-11-24T01:26:03ZengMDPI AGHorticulturae2311-75242022-03-018325210.3390/horticulturae8030252Protein Extract of Tobacco Expressing <i>Solanum torvum</i> PP5-Encoding Gene Inhibits <i>Verticillium dahliae</i> ProliferationMin Chen0Chao Xie1Qing Yang2Wenjiao Zhu3Man Wang4College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Life Sciences, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Life Sciences, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Life Sciences, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Life Sciences, Nanjing Agricultural University, Nanjing 210095, ChinaVerticillium wilt, a soilborne disease caused by <i>Verticillium dahliae</i> (<i>V. dahliae</i>), can severely affect the yields of Solanaceae crops. In a previous study, it was observed in <i>Solanum torvum</i> (<i>S. torvum</i>) that protein phosphatase 5 (PP5) was induced by <i>V. dahliae</i> infection. To elucidate the function of PP5 more clearly, this study cloned an <i>StPP5</i> cDNA from <i>S. torvum</i> by PCR. The cDNA contained an ORF of 1458 bp long encoding a putative protein of 485 amino acid residues with a predicted molecular mass of 54.63 kDa and a theoretical isoelectric point of 5.66. StPP5 protein contained a conserved PP domain and showed high similarity to other homologous members of the PP5 family from various plant species. The expression of <i>StPP5</i> gene was upregulated after <i>V.</i> infection and reached its maximum value at 24 h in leaves. In order to clarify the role of <i>StPP5</i>, four transgenic tobacco plants expressing <i>StPP5</i> were generated through <i>Agrobacterium</i>-mediated transformation and identified by PCR. In vitro culture assay showed that the growth of <i>V. dahliae</i> in PDA medium containing proteins extracted from the leaves of transgenic tobacco line P6 was inhibited, whose inhibition rate was 55.1%, higher than the non-transgenic control. These results indicated that StPP5 might be involved in plant defense against <i>V. dahliae</i> infection.https://www.mdpi.com/2311-7524/8/3/252<i>Solanum torvum</i><i>StPP5</i>gene cloningfunction analysis |
| spellingShingle | Min Chen Chao Xie Qing Yang Wenjiao Zhu Man Wang Protein Extract of Tobacco Expressing <i>Solanum torvum</i> PP5-Encoding Gene Inhibits <i>Verticillium dahliae</i> Proliferation Horticulturae <i>Solanum torvum</i> <i>StPP5</i> gene cloning function analysis |
| title | Protein Extract of Tobacco Expressing <i>Solanum torvum</i> PP5-Encoding Gene Inhibits <i>Verticillium dahliae</i> Proliferation |
| title_full | Protein Extract of Tobacco Expressing <i>Solanum torvum</i> PP5-Encoding Gene Inhibits <i>Verticillium dahliae</i> Proliferation |
| title_fullStr | Protein Extract of Tobacco Expressing <i>Solanum torvum</i> PP5-Encoding Gene Inhibits <i>Verticillium dahliae</i> Proliferation |
| title_full_unstemmed | Protein Extract of Tobacco Expressing <i>Solanum torvum</i> PP5-Encoding Gene Inhibits <i>Verticillium dahliae</i> Proliferation |
| title_short | Protein Extract of Tobacco Expressing <i>Solanum torvum</i> PP5-Encoding Gene Inhibits <i>Verticillium dahliae</i> Proliferation |
| title_sort | protein extract of tobacco expressing i solanum torvum i pp5 encoding gene inhibits i verticillium dahliae i proliferation |
| topic | <i>Solanum torvum</i> <i>StPP5</i> gene cloning function analysis |
| url | https://www.mdpi.com/2311-7524/8/3/252 |
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