QTL Mapping and Identification of Candidate Genes for Heat Tolerance at the Flowering Stage in Rice
High-temperature stress can cause serious abiotic damage that limits the yield and quality of rice. Heat tolerance (HT) during the flowering stage of rice is a key trait that can guarantee a high and stable yield under heat stress. HT is a complex trait that is regulated by multiple quantitative tra...
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Frontiers Media S.A.
2021-01-01
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| Series: | Frontiers in Genetics |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fgene.2020.621871/full |
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| author | Lei Chen Lei Chen Qiang Wang Maoyan Tang Xiaoli Zhang Yinghua Pan Xinghai Yang Guoqing Gao Ronghua Lv Wei Tao Ligeng Jiang Tianfeng Liang |
| author_facet | Lei Chen Lei Chen Qiang Wang Maoyan Tang Xiaoli Zhang Yinghua Pan Xinghai Yang Guoqing Gao Ronghua Lv Wei Tao Ligeng Jiang Tianfeng Liang |
| author_sort | Lei Chen |
| collection | DOAJ |
| description | High-temperature stress can cause serious abiotic damage that limits the yield and quality of rice. Heat tolerance (HT) during the flowering stage of rice is a key trait that can guarantee a high and stable yield under heat stress. HT is a complex trait that is regulated by multiple quantitative trait loci (QTLs); however, few underlying genes have been fine mapped and cloned. In this study, the F2:3 population derived from a cross between Huanghuazhan (HHZ), a heat-tolerant cultivar, and 9311, a heat-sensitive variety, was used to map HT QTLs during the flowering stage in rice. A new major QTL, qHTT8, controlling HT was identified on chromosome 8 using the bulked-segregant analysis (BSA)-seq method. The QTL qHTT8 was mapped into the 3,555,000–4,520,000 bp, which had a size of 0.965 Mb. The candidate region of qHTT8 on chromosome 8 contained 65 predicted genes, and 10 putative predicted genes were found to be associated with abiotic stress tolerance. Furthermore, qRT-PCR was performed to analyze the differential expression of these 10 genes between HHZ and 9311 under high temperature conditions. LOC_Os08g07010 and LOC_Os08g07440 were highly induced in HHZ compared with 9311 under heat stress. Orthologous genes of LOC_Os08g07010 and LOC_Os08g07440 in plants played a role in abiotic stress, suggesting that they may be the candidate genes of qHTT8. Generally, the results of this study will prove useful for future efforts to clone qHTT8 and breed heat-tolerant varieties of rice using marker-assisted selection. |
| first_indexed | 2024-12-14T06:00:25Z |
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| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-12-14T06:00:25Z |
| publishDate | 2021-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj.art-53ca9f9c6c714f829fb358858eff43f32022-12-21T23:14:27ZengFrontiers Media S.A.Frontiers in Genetics1664-80212021-01-011110.3389/fgene.2020.621871621871QTL Mapping and Identification of Candidate Genes for Heat Tolerance at the Flowering Stage in RiceLei Chen0Lei Chen1Qiang Wang2Maoyan Tang3Xiaoli Zhang4Yinghua Pan5Xinghai Yang6Guoqing Gao7Ronghua Lv8Wei Tao9Ligeng Jiang10Tianfeng Liang11Key Laboratory of Crop Cultivation and Farming System, College of Agriculture, Guangxi University, Nanning, ChinaGuangxi Academy of Agricultural Sciences/Guangxi Key Laboratory of Rice Genetics and Breeding, Rice Research Institute, Nanning, ChinaGuangxi Academy of Agricultural Sciences/Guangxi Key Laboratory of Rice Genetics and Breeding, Rice Research Institute, Nanning, ChinaGuangxi Academy of Agricultural Sciences/Guangxi Key Laboratory of Rice Genetics and Breeding, Rice Research Institute, Nanning, ChinaGuangxi Academy of Agricultural Sciences/Guangxi Key Laboratory of Rice Genetics and Breeding, Rice Research Institute, Nanning, ChinaGuangxi Academy of Agricultural Sciences/Guangxi Key Laboratory of Rice Genetics and Breeding, Rice Research Institute, Nanning, ChinaGuangxi Academy of Agricultural Sciences/Guangxi Key Laboratory of Rice Genetics and Breeding, Rice Research Institute, Nanning, ChinaGuangxi Academy of Agricultural Sciences/Guangxi Key Laboratory of Rice Genetics and Breeding, Rice Research Institute, Nanning, ChinaGuangxi Academy of Agricultural Sciences/Guangxi Key Laboratory of Rice Genetics and Breeding, Rice Research Institute, Nanning, ChinaGuangxi Academy of Agricultural Sciences/Guangxi Key Laboratory of Rice Genetics and Breeding, Rice Research Institute, Nanning, ChinaKey Laboratory of Crop Cultivation and Farming System, College of Agriculture, Guangxi University, Nanning, ChinaGuangxi Academy of Agricultural Sciences/Guangxi Key Laboratory of Rice Genetics and Breeding, Rice Research Institute, Nanning, ChinaHigh-temperature stress can cause serious abiotic damage that limits the yield and quality of rice. Heat tolerance (HT) during the flowering stage of rice is a key trait that can guarantee a high and stable yield under heat stress. HT is a complex trait that is regulated by multiple quantitative trait loci (QTLs); however, few underlying genes have been fine mapped and cloned. In this study, the F2:3 population derived from a cross between Huanghuazhan (HHZ), a heat-tolerant cultivar, and 9311, a heat-sensitive variety, was used to map HT QTLs during the flowering stage in rice. A new major QTL, qHTT8, controlling HT was identified on chromosome 8 using the bulked-segregant analysis (BSA)-seq method. The QTL qHTT8 was mapped into the 3,555,000–4,520,000 bp, which had a size of 0.965 Mb. The candidate region of qHTT8 on chromosome 8 contained 65 predicted genes, and 10 putative predicted genes were found to be associated with abiotic stress tolerance. Furthermore, qRT-PCR was performed to analyze the differential expression of these 10 genes between HHZ and 9311 under high temperature conditions. LOC_Os08g07010 and LOC_Os08g07440 were highly induced in HHZ compared with 9311 under heat stress. Orthologous genes of LOC_Os08g07010 and LOC_Os08g07440 in plants played a role in abiotic stress, suggesting that they may be the candidate genes of qHTT8. Generally, the results of this study will prove useful for future efforts to clone qHTT8 and breed heat-tolerant varieties of rice using marker-assisted selection.https://www.frontiersin.org/articles/10.3389/fgene.2020.621871/fullriceflowering stageheat toleranceQTLBSA-seqcandidate gene |
| spellingShingle | Lei Chen Lei Chen Qiang Wang Maoyan Tang Xiaoli Zhang Yinghua Pan Xinghai Yang Guoqing Gao Ronghua Lv Wei Tao Ligeng Jiang Tianfeng Liang QTL Mapping and Identification of Candidate Genes for Heat Tolerance at the Flowering Stage in Rice Frontiers in Genetics rice flowering stage heat tolerance QTL BSA-seq candidate gene |
| title | QTL Mapping and Identification of Candidate Genes for Heat Tolerance at the Flowering Stage in Rice |
| title_full | QTL Mapping and Identification of Candidate Genes for Heat Tolerance at the Flowering Stage in Rice |
| title_fullStr | QTL Mapping and Identification of Candidate Genes for Heat Tolerance at the Flowering Stage in Rice |
| title_full_unstemmed | QTL Mapping and Identification of Candidate Genes for Heat Tolerance at the Flowering Stage in Rice |
| title_short | QTL Mapping and Identification of Candidate Genes for Heat Tolerance at the Flowering Stage in Rice |
| title_sort | qtl mapping and identification of candidate genes for heat tolerance at the flowering stage in rice |
| topic | rice flowering stage heat tolerance QTL BSA-seq candidate gene |
| url | https://www.frontiersin.org/articles/10.3389/fgene.2020.621871/full |
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