Diesel exhaust particle exposure in vitro alters monocyte differentiation and function.
Air pollution by diesel exhaust particles is associated with elevated mortality and increased hospital admissions in individuals with respiratory diseases such as asthma and chronic obstructive pulmonary disease. During active inflammation monocytes are recruited to the airways and can replace resid...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2012-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3517601?pdf=render |
| _version_ | 1819085414573539328 |
|---|---|
| author | Nazia Chaudhuri Hannah Jary Simon Lea Naimat Khan Katie C Piddock David H Dockrell Ken Donaldson Rodger Duffin Dave Singh Lisa C Parker Ian Sabroe |
| author_facet | Nazia Chaudhuri Hannah Jary Simon Lea Naimat Khan Katie C Piddock David H Dockrell Ken Donaldson Rodger Duffin Dave Singh Lisa C Parker Ian Sabroe |
| author_sort | Nazia Chaudhuri |
| collection | DOAJ |
| description | Air pollution by diesel exhaust particles is associated with elevated mortality and increased hospital admissions in individuals with respiratory diseases such as asthma and chronic obstructive pulmonary disease. During active inflammation monocytes are recruited to the airways and can replace resident alveolar macrophages. We therefore investigated whether chronic fourteen day exposure to low concentrations of diesel exhaust particles can alter the phenotype and function of monocytes from healthy individuals and those with chronic obstructive pulmonary disease. Monocytes were purified from the blood of healthy individuals and people with a diagnosis of chronic obstructive pulmonary disease. Monocyte-derived macrophages were generated in the presence or absence of diesel exhaust particles and their phenotypes studied through investigation of their lifespan, cytokine generation in response to Toll like receptor agonists and heat killed bacteria, and expression of surface markers. Chronic fourteen day exposure of monocyte-derived macrophages to concentrations of diesel exhaust particles >10 µg/ml caused mitochondrial and lysosomal dysfunction, and a gradual loss of cells over time both in healthy and chronic obstructive pulmonary disease individuals. Chronic exposure to lower concentrations of diesel exhaust particles impaired CXCL8 cytokine responses to lipopolysaccharide and heat killed E. coli, and this phenotype was associated with a reduction in CD14 and CD11b expression. Chronic diesel exhaust particle exposure may therefore alter both numbers and function of lung macrophages differentiating from locally recruited monocytes in the lungs of healthy people and patients with chronic obstructive pulmonary disease. |
| first_indexed | 2024-12-21T21:03:59Z |
| format | Article |
| id | doaj.art-53ce29bdf5204f6f87ad4f90c052b5aa |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-21T21:03:59Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-53ce29bdf5204f6f87ad4f90c052b5aa2022-12-21T18:50:21ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-01712e5110710.1371/journal.pone.0051107Diesel exhaust particle exposure in vitro alters monocyte differentiation and function.Nazia ChaudhuriHannah JarySimon LeaNaimat KhanKatie C PiddockDavid H DockrellKen DonaldsonRodger DuffinDave SinghLisa C ParkerIan SabroeAir pollution by diesel exhaust particles is associated with elevated mortality and increased hospital admissions in individuals with respiratory diseases such as asthma and chronic obstructive pulmonary disease. During active inflammation monocytes are recruited to the airways and can replace resident alveolar macrophages. We therefore investigated whether chronic fourteen day exposure to low concentrations of diesel exhaust particles can alter the phenotype and function of monocytes from healthy individuals and those with chronic obstructive pulmonary disease. Monocytes were purified from the blood of healthy individuals and people with a diagnosis of chronic obstructive pulmonary disease. Monocyte-derived macrophages were generated in the presence or absence of diesel exhaust particles and their phenotypes studied through investigation of their lifespan, cytokine generation in response to Toll like receptor agonists and heat killed bacteria, and expression of surface markers. Chronic fourteen day exposure of monocyte-derived macrophages to concentrations of diesel exhaust particles >10 µg/ml caused mitochondrial and lysosomal dysfunction, and a gradual loss of cells over time both in healthy and chronic obstructive pulmonary disease individuals. Chronic exposure to lower concentrations of diesel exhaust particles impaired CXCL8 cytokine responses to lipopolysaccharide and heat killed E. coli, and this phenotype was associated with a reduction in CD14 and CD11b expression. Chronic diesel exhaust particle exposure may therefore alter both numbers and function of lung macrophages differentiating from locally recruited monocytes in the lungs of healthy people and patients with chronic obstructive pulmonary disease.http://europepmc.org/articles/PMC3517601?pdf=render |
| spellingShingle | Nazia Chaudhuri Hannah Jary Simon Lea Naimat Khan Katie C Piddock David H Dockrell Ken Donaldson Rodger Duffin Dave Singh Lisa C Parker Ian Sabroe Diesel exhaust particle exposure in vitro alters monocyte differentiation and function. PLoS ONE |
| title | Diesel exhaust particle exposure in vitro alters monocyte differentiation and function. |
| title_full | Diesel exhaust particle exposure in vitro alters monocyte differentiation and function. |
| title_fullStr | Diesel exhaust particle exposure in vitro alters monocyte differentiation and function. |
| title_full_unstemmed | Diesel exhaust particle exposure in vitro alters monocyte differentiation and function. |
| title_short | Diesel exhaust particle exposure in vitro alters monocyte differentiation and function. |
| title_sort | diesel exhaust particle exposure in vitro alters monocyte differentiation and function |
| url | http://europepmc.org/articles/PMC3517601?pdf=render |
| work_keys_str_mv | AT naziachaudhuri dieselexhaustparticleexposureinvitroaltersmonocytedifferentiationandfunction AT hannahjary dieselexhaustparticleexposureinvitroaltersmonocytedifferentiationandfunction AT simonlea dieselexhaustparticleexposureinvitroaltersmonocytedifferentiationandfunction AT naimatkhan dieselexhaustparticleexposureinvitroaltersmonocytedifferentiationandfunction AT katiecpiddock dieselexhaustparticleexposureinvitroaltersmonocytedifferentiationandfunction AT davidhdockrell dieselexhaustparticleexposureinvitroaltersmonocytedifferentiationandfunction AT kendonaldson dieselexhaustparticleexposureinvitroaltersmonocytedifferentiationandfunction AT rodgerduffin dieselexhaustparticleexposureinvitroaltersmonocytedifferentiationandfunction AT davesingh dieselexhaustparticleexposureinvitroaltersmonocytedifferentiationandfunction AT lisacparker dieselexhaustparticleexposureinvitroaltersmonocytedifferentiationandfunction AT iansabroe dieselexhaustparticleexposureinvitroaltersmonocytedifferentiationandfunction |