Transcriptomic Analysis of Yunwu Tribute Tea Leaves under Cold Stress
Background: Cold stress usually occurs in winter and is one of the most significant environmental factors restricting the growth of the tea plant as well as its geographical distribution. Objective: It is necessary to identify the physiological and molecular mechanisms of plants under cold stress so...
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MDPI AG
2023-01-01
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| Series: | Current Issues in Molecular Biology |
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| Online Access: | https://www.mdpi.com/1467-3045/45/1/47 |
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| author | Ying Wang Cheng Wan Leijia Li Zhun Xiang Jihong Wang Yan Li Degang Zhao |
| author_facet | Ying Wang Cheng Wan Leijia Li Zhun Xiang Jihong Wang Yan Li Degang Zhao |
| author_sort | Ying Wang |
| collection | DOAJ |
| description | Background: Cold stress usually occurs in winter and is one of the most significant environmental factors restricting the growth of the tea plant as well as its geographical distribution. Objective: It is necessary to identify the physiological and molecular mechanisms of plants under cold stress so that cold-tolerant crop varieties can be cultivated to limit production losses. At the same time, this would allow the crop planting area to be expanded, hence improving the economic benefits. Methods: In this study, the transcriptome data of Yunwu Tribute Tea under cold conditions were obtained using the Illumina HiSeq platform. By analyzing changes in transcriptome data associated with the antioxidant enzyme system, plant hormone signal transduction, proline and tyrosine metabolism pathways, and transcription factors, the molecular mechanisms involved in Yunwu Tribute Tea under cold stress were investigated. Results: In this study, Illumina HiSeq technology was applied to investigate the cold-tolerance mechanism. For this purpose, cDNA libraries were obtained from two groups of samples, namely the cold-treated group (DW) and the control group (CK). A total of 185,973 unigenes were produced from 511,987 assembled transcripts; among these, 16,020 differentially expressed genes (DEGs) (corrected <i>p</i>-value < 0.01, |log2(fold change)| >3), including 9606 up-regulated and 6414 down-regulated genes, were obtained. Moreover, the antioxidant enzyme system, plant hormone signal transduction, proline and tyrosine metabolism pathways, and transcription factors were analyzed; based on these results, a series of candidate genes related to cold stress were screened out and discussed. The physiological indexes related to the low-temperature response were tested, along with five DEGs which were validated by quantitative real-time PCR. Conclusions: Differential gene expression analysis has confirmed that substantial cold-responsive genes are related to the antioxidant enzyme system, plant hormone signal transduction, proline metabolism pathway, tyrosine metabolism pathway, and transcription factors. |
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| spelling | doaj.art-53cef997215f421f9ffbf372dc2be6192023-11-30T21:45:39ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452023-01-0145169972010.3390/cimb45010047Transcriptomic Analysis of Yunwu Tribute Tea Leaves under Cold StressYing Wang0Cheng Wan1Leijia Li2Zhun Xiang3Jihong Wang4Yan Li5Degang Zhao6Guizhou Province Institute of Biology, Guizhou Academy of Sciences, Guiyang 550009, ChinaGuizhou Province Institute of Biology, Guizhou Academy of Sciences, Guiyang 550009, ChinaGuizhou Province Institute of Biology, Guizhou Academy of Sciences, Guiyang 550009, ChinaGuizhou Province Institute of Biology, Guizhou Academy of Sciences, Guiyang 550009, ChinaGuizhou Province Institute of Biology, Guizhou Academy of Sciences, Guiyang 550009, ChinaThe Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), Guizhou University, Guiyang 550025, ChinaThe Application Center for Plant Conservation Technology, Guizhou Academy of Agricultural Sciences, Guiyang 550006, ChinaBackground: Cold stress usually occurs in winter and is one of the most significant environmental factors restricting the growth of the tea plant as well as its geographical distribution. Objective: It is necessary to identify the physiological and molecular mechanisms of plants under cold stress so that cold-tolerant crop varieties can be cultivated to limit production losses. At the same time, this would allow the crop planting area to be expanded, hence improving the economic benefits. Methods: In this study, the transcriptome data of Yunwu Tribute Tea under cold conditions were obtained using the Illumina HiSeq platform. By analyzing changes in transcriptome data associated with the antioxidant enzyme system, plant hormone signal transduction, proline and tyrosine metabolism pathways, and transcription factors, the molecular mechanisms involved in Yunwu Tribute Tea under cold stress were investigated. Results: In this study, Illumina HiSeq technology was applied to investigate the cold-tolerance mechanism. For this purpose, cDNA libraries were obtained from two groups of samples, namely the cold-treated group (DW) and the control group (CK). A total of 185,973 unigenes were produced from 511,987 assembled transcripts; among these, 16,020 differentially expressed genes (DEGs) (corrected <i>p</i>-value < 0.01, |log2(fold change)| >3), including 9606 up-regulated and 6414 down-regulated genes, were obtained. Moreover, the antioxidant enzyme system, plant hormone signal transduction, proline and tyrosine metabolism pathways, and transcription factors were analyzed; based on these results, a series of candidate genes related to cold stress were screened out and discussed. The physiological indexes related to the low-temperature response were tested, along with five DEGs which were validated by quantitative real-time PCR. Conclusions: Differential gene expression analysis has confirmed that substantial cold-responsive genes are related to the antioxidant enzyme system, plant hormone signal transduction, proline metabolism pathway, tyrosine metabolism pathway, and transcription factors.https://www.mdpi.com/1467-3045/45/1/47transcriptomecold stressmetabolic pathways<i>Camellia sinensis</i> (L.) Kuntze var. <i>niaowangensis</i> Q. H. Chen |
| spellingShingle | Ying Wang Cheng Wan Leijia Li Zhun Xiang Jihong Wang Yan Li Degang Zhao Transcriptomic Analysis of Yunwu Tribute Tea Leaves under Cold Stress Current Issues in Molecular Biology transcriptome cold stress metabolic pathways <i>Camellia sinensis</i> (L.) Kuntze var. <i>niaowangensis</i> Q. H. Chen |
| title | Transcriptomic Analysis of Yunwu Tribute Tea Leaves under Cold Stress |
| title_full | Transcriptomic Analysis of Yunwu Tribute Tea Leaves under Cold Stress |
| title_fullStr | Transcriptomic Analysis of Yunwu Tribute Tea Leaves under Cold Stress |
| title_full_unstemmed | Transcriptomic Analysis of Yunwu Tribute Tea Leaves under Cold Stress |
| title_short | Transcriptomic Analysis of Yunwu Tribute Tea Leaves under Cold Stress |
| title_sort | transcriptomic analysis of yunwu tribute tea leaves under cold stress |
| topic | transcriptome cold stress metabolic pathways <i>Camellia sinensis</i> (L.) Kuntze var. <i>niaowangensis</i> Q. H. Chen |
| url | https://www.mdpi.com/1467-3045/45/1/47 |
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