Genetic diversity analysis in the section Caulorrhizae (genus Arachis) using microsatellite markers
Diversity in 26 microsatellite loci from section Caulorrhizae germplasm was evaluated by using 33 accessions of A. pintoi Krapov. & W.C. Gregory and ten accessions of Arachis repens Handro. Twenty loci proved to be polymorphic and a total of 196 alleles were detected with an average of 9.8 allel...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Genética
2010-01-01
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| Series: | Genetics and Molecular Biology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100019 |
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| author | Darío A. Palmieri Marcelo D. Bechara Rogério A. Curi Jomar P. Monteiro Sérgio E.S. Valente Marcos A. Gimenes Catalina R. Lopes |
| author_facet | Darío A. Palmieri Marcelo D. Bechara Rogério A. Curi Jomar P. Monteiro Sérgio E.S. Valente Marcos A. Gimenes Catalina R. Lopes |
| author_sort | Darío A. Palmieri |
| collection | DOAJ |
| description | Diversity in 26 microsatellite loci from section Caulorrhizae germplasm was evaluated by using 33 accessions of A. pintoi Krapov. & W.C. Gregory and ten accessions of Arachis repens Handro. Twenty loci proved to be polymorphic and a total of 196 alleles were detected with an average of 9.8 alleles per locus. The variability found in those loci was greater than the variability found using morphological characters, seed storage proteins and RAPD markers previously used in this germplasm. The high potential of these markers to detect species-specific alleles and discriminate among accessions was demonstrated. The set of microsatellite primer pairs developed by our group for A. pintoi are useful molecular tools for evaluating Section Caulorrhizae germplasm, as well as that of species belonging to other Arachis sections. |
| first_indexed | 2024-04-13T08:48:46Z |
| format | Article |
| id | doaj.art-53cf333fef2c46778ca327a45bcde0e8 |
| institution | Directory Open Access Journal |
| issn | 1415-4757 1678-4685 |
| language | English |
| last_indexed | 2024-04-13T08:48:46Z |
| publishDate | 2010-01-01 |
| publisher | Sociedade Brasileira de Genética |
| record_format | Article |
| series | Genetics and Molecular Biology |
| spelling | doaj.art-53cf333fef2c46778ca327a45bcde0e82022-12-22T02:53:35ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1415-47571678-46852010-01-01331109118Genetic diversity analysis in the section Caulorrhizae (genus Arachis) using microsatellite markersDarío A. PalmieriMarcelo D. BecharaRogério A. CuriJomar P. MonteiroSérgio E.S. ValenteMarcos A. GimenesCatalina R. LopesDiversity in 26 microsatellite loci from section Caulorrhizae germplasm was evaluated by using 33 accessions of A. pintoi Krapov. & W.C. Gregory and ten accessions of Arachis repens Handro. Twenty loci proved to be polymorphic and a total of 196 alleles were detected with an average of 9.8 alleles per locus. The variability found in those loci was greater than the variability found using morphological characters, seed storage proteins and RAPD markers previously used in this germplasm. The high potential of these markers to detect species-specific alleles and discriminate among accessions was demonstrated. The set of microsatellite primer pairs developed by our group for A. pintoi are useful molecular tools for evaluating Section Caulorrhizae germplasm, as well as that of species belonging to other Arachis sections.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100019Arachisgenetic diversitygermplasmmicrosatellitesmolecular markers |
| spellingShingle | Darío A. Palmieri Marcelo D. Bechara Rogério A. Curi Jomar P. Monteiro Sérgio E.S. Valente Marcos A. Gimenes Catalina R. Lopes Genetic diversity analysis in the section Caulorrhizae (genus Arachis) using microsatellite markers Genetics and Molecular Biology Arachis genetic diversity germplasm microsatellites molecular markers |
| title | Genetic diversity analysis in the section Caulorrhizae (genus Arachis) using microsatellite markers |
| title_full | Genetic diversity analysis in the section Caulorrhizae (genus Arachis) using microsatellite markers |
| title_fullStr | Genetic diversity analysis in the section Caulorrhizae (genus Arachis) using microsatellite markers |
| title_full_unstemmed | Genetic diversity analysis in the section Caulorrhizae (genus Arachis) using microsatellite markers |
| title_short | Genetic diversity analysis in the section Caulorrhizae (genus Arachis) using microsatellite markers |
| title_sort | genetic diversity analysis in the section caulorrhizae genus arachis using microsatellite markers |
| topic | Arachis genetic diversity germplasm microsatellites molecular markers |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100019 |
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