Meristem culture for the elimination of the virus S of the potato in plants cultivated <i>in vitro</i>
The virus S of potato was detected in three varieties of scientific and productive interests during propagation in vitro of different genotypes. With the objective of obtaining plants free of virus, three sizes of meristems (0.3 to 0.2mm, 0.2 to 0.1mm, and 0.1 to 0.06mm) were studied. For the growth...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Universidad Central Marta Abreu de Las Villas
2001-04-01
|
| Series: | Biotecnología Vegetal |
| Online Access: | https://revista.ibp.co.cu/index.php/BV/article/view/77 |
| _version_ | 1818068064992755712 |
|---|---|
| author | Leyanis García-Águila Zoe Sarría Tatiana Pichardo Blanca Pérez |
| author_facet | Leyanis García-Águila Zoe Sarría Tatiana Pichardo Blanca Pérez |
| author_sort | Leyanis García-Águila |
| collection | DOAJ |
| description | The virus S of potato was detected in three varieties of scientific and productive interests during propagation in vitro of different genotypes. With the objective of obtaining plants free of virus, three sizes of meristems (0.3 to 0.2mm, 0.2 to 0.1mm, and 0.1 to 0.06mm) were studied. For the growth of the meristems a culture medium was used that contained Murashige and Skoog (1962) salts, 1 mg.l-1 thyamine, 100 mg.l-1 myoinositol, 1 mg.l-1 gibberelic acid and 1.8 mg.l-1 gelling agent. The plants regenerated from meristems and after two transfers to multiplication culture medium were diagnosed through the ELISA technique. As a result 96.6% of cleaness was obtained when the range of meristem size was from 0.1 to 0.06 mm. The regeneration percentages of plants from the meristems decreased, as the range of size was smaller.
Key words: regeneration of plants, Solanum tuberosum L, virus infection, virus free plants |
| first_indexed | 2024-12-10T15:33:39Z |
| format | Article |
| id | doaj.art-53cfd05ac0d74484b040cc8f61e6828c |
| institution | Directory Open Access Journal |
| issn | 1609-1841 2074-8647 |
| language | English |
| last_indexed | 2024-12-10T15:33:39Z |
| publishDate | 2001-04-01 |
| publisher | Universidad Central Marta Abreu de Las Villas |
| record_format | Article |
| series | Biotecnología Vegetal |
| spelling | doaj.art-53cfd05ac0d74484b040cc8f61e6828c2022-12-22T01:43:19ZengUniversidad Central Marta Abreu de Las VillasBiotecnología Vegetal1609-18412074-86472001-04-011265Meristem culture for the elimination of the virus S of the potato in plants cultivated <i>in vitro</i>Leyanis García-ÁguilaZoe SarríaTatiana PichardoBlanca PérezThe virus S of potato was detected in three varieties of scientific and productive interests during propagation in vitro of different genotypes. With the objective of obtaining plants free of virus, three sizes of meristems (0.3 to 0.2mm, 0.2 to 0.1mm, and 0.1 to 0.06mm) were studied. For the growth of the meristems a culture medium was used that contained Murashige and Skoog (1962) salts, 1 mg.l-1 thyamine, 100 mg.l-1 myoinositol, 1 mg.l-1 gibberelic acid and 1.8 mg.l-1 gelling agent. The plants regenerated from meristems and after two transfers to multiplication culture medium were diagnosed through the ELISA technique. As a result 96.6% of cleaness was obtained when the range of meristem size was from 0.1 to 0.06 mm. The regeneration percentages of plants from the meristems decreased, as the range of size was smaller. Key words: regeneration of plants, Solanum tuberosum L, virus infection, virus free plantshttps://revista.ibp.co.cu/index.php/BV/article/view/77 |
| spellingShingle | Leyanis García-Águila Zoe Sarría Tatiana Pichardo Blanca Pérez Meristem culture for the elimination of the virus S of the potato in plants cultivated <i>in vitro</i> Biotecnología Vegetal |
| title | Meristem culture for the elimination of the virus S of the potato in plants cultivated <i>in vitro</i> |
| title_full | Meristem culture for the elimination of the virus S of the potato in plants cultivated <i>in vitro</i> |
| title_fullStr | Meristem culture for the elimination of the virus S of the potato in plants cultivated <i>in vitro</i> |
| title_full_unstemmed | Meristem culture for the elimination of the virus S of the potato in plants cultivated <i>in vitro</i> |
| title_short | Meristem culture for the elimination of the virus S of the potato in plants cultivated <i>in vitro</i> |
| title_sort | meristem culture for the elimination of the virus s of the potato in plants cultivated i in vitro i |
| url | https://revista.ibp.co.cu/index.php/BV/article/view/77 |
| work_keys_str_mv | AT leyanisgarciaaguila meristemculturefortheeliminationofthevirussofthepotatoinplantscultivatediinvitroi AT zoesarria meristemculturefortheeliminationofthevirussofthepotatoinplantscultivatediinvitroi AT tatianapichardo meristemculturefortheeliminationofthevirussofthepotatoinplantscultivatediinvitroi AT blancaperez meristemculturefortheeliminationofthevirussofthepotatoinplantscultivatediinvitroi |