Identification of Differentially Expressed MicroRNAs and Their Potential Target Genes in Adipose Tissue from Pigs with Highly Divergent Backfat Thickness
Fatty traits are very important in pig production. However, the role of microRNAs (miRNAs) in fat deposition is not clearly understood. In this study, we compared adipose miRNAs from three full-sibling pairs of female Landrace pigs, with high and low backfat thickness, to investigate the associated...
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| Format: | Article |
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MDPI AG
2020-04-01
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| Online Access: | https://www.mdpi.com/2076-2615/10/4/624 |
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| author | Kai Xing Xitong Zhao Yibing Liu Fengxia Zhang Zhen Tan Xiaolong Qi Xiangguo Wang Hemin Ni Yong Guo Xihui Sheng Chuduan Wang |
| author_facet | Kai Xing Xitong Zhao Yibing Liu Fengxia Zhang Zhen Tan Xiaolong Qi Xiangguo Wang Hemin Ni Yong Guo Xihui Sheng Chuduan Wang |
| author_sort | Kai Xing |
| collection | DOAJ |
| description | Fatty traits are very important in pig production. However, the role of microRNAs (miRNAs) in fat deposition is not clearly understood. In this study, we compared adipose miRNAs from three full-sibling pairs of female Landrace pigs, with high and low backfat thickness, to investigate the associated regulatory network. We obtained an average of 17.29 million raw reads from six libraries, 62.27% of which mapped to the pig reference genome. A total of 318 pig miRNAs were detected among the samples. Among them, 18 miRNAs were differentially expressed (<i>p</i>-value < 0.05, |log<sub>2</sub>fold change| ≥ 1) between the high and low backfat groups; 6 were up-regulated and 12 were down-regulated. Functional enrichment of the predicted target genes of the differentially expressed miRNAs, indicated that these miRNAs were involved mainly in lipid and carbohydrate metabolism, and glycan biosynthesis and metabolism. Comprehensive analysis of the mRNA and miRNA transcriptomes revealed possible regulatory relationships for fat deposition. Negatively correlated mRNA–miRNA pairs included miR-137–<i>PPARGC1A</i>, miR-141–<i>FASN</i>, and miR-122-5p–<i>PKM</i>, indicating these interactions may be key regulators of fat deposition. Our findings provide important insights into miRNA expression patterns in the backfat tissue of pig and new insights into the regulatory mechanisms of fat deposition in pig. |
| first_indexed | 2024-03-10T20:39:47Z |
| format | Article |
| id | doaj.art-53d0a20320214330bad7703e2e615940 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T20:39:47Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-53d0a20320214330bad7703e2e6159402023-11-19T20:45:45ZengMDPI AGAnimals2076-26152020-04-0110462410.3390/ani10040624Identification of Differentially Expressed MicroRNAs and Their Potential Target Genes in Adipose Tissue from Pigs with Highly Divergent Backfat ThicknessKai Xing0Xitong Zhao1Yibing Liu2Fengxia Zhang3Zhen Tan4Xiaolong Qi5Xiangguo Wang6Hemin Ni7Yong Guo8Xihui Sheng9Chuduan Wang10Animal Science and Technology College, Beijing University of Agriculture, Beijing 102206, ChinaKey Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture, National Engineering laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, ChinaKey Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture, National Engineering laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, ChinaKey Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture, National Engineering laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, ChinaCollege of Animal Science and Technology, College of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, ChinaAnimal Science and Technology College, Beijing University of Agriculture, Beijing 102206, ChinaAnimal Science and Technology College, Beijing University of Agriculture, Beijing 102206, ChinaAnimal Science and Technology College, Beijing University of Agriculture, Beijing 102206, ChinaAnimal Science and Technology College, Beijing University of Agriculture, Beijing 102206, ChinaAnimal Science and Technology College, Beijing University of Agriculture, Beijing 102206, ChinaKey Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture, National Engineering laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, ChinaFatty traits are very important in pig production. However, the role of microRNAs (miRNAs) in fat deposition is not clearly understood. In this study, we compared adipose miRNAs from three full-sibling pairs of female Landrace pigs, with high and low backfat thickness, to investigate the associated regulatory network. We obtained an average of 17.29 million raw reads from six libraries, 62.27% of which mapped to the pig reference genome. A total of 318 pig miRNAs were detected among the samples. Among them, 18 miRNAs were differentially expressed (<i>p</i>-value < 0.05, |log<sub>2</sub>fold change| ≥ 1) between the high and low backfat groups; 6 were up-regulated and 12 were down-regulated. Functional enrichment of the predicted target genes of the differentially expressed miRNAs, indicated that these miRNAs were involved mainly in lipid and carbohydrate metabolism, and glycan biosynthesis and metabolism. Comprehensive analysis of the mRNA and miRNA transcriptomes revealed possible regulatory relationships for fat deposition. Negatively correlated mRNA–miRNA pairs included miR-137–<i>PPARGC1A</i>, miR-141–<i>FASN</i>, and miR-122-5p–<i>PKM</i>, indicating these interactions may be key regulators of fat deposition. Our findings provide important insights into miRNA expression patterns in the backfat tissue of pig and new insights into the regulatory mechanisms of fat deposition in pig.https://www.mdpi.com/2076-2615/10/4/624pigmiRNAfat depositionregulatory network |
| spellingShingle | Kai Xing Xitong Zhao Yibing Liu Fengxia Zhang Zhen Tan Xiaolong Qi Xiangguo Wang Hemin Ni Yong Guo Xihui Sheng Chuduan Wang Identification of Differentially Expressed MicroRNAs and Their Potential Target Genes in Adipose Tissue from Pigs with Highly Divergent Backfat Thickness Animals pig miRNA fat deposition regulatory network |
| title | Identification of Differentially Expressed MicroRNAs and Their Potential Target Genes in Adipose Tissue from Pigs with Highly Divergent Backfat Thickness |
| title_full | Identification of Differentially Expressed MicroRNAs and Their Potential Target Genes in Adipose Tissue from Pigs with Highly Divergent Backfat Thickness |
| title_fullStr | Identification of Differentially Expressed MicroRNAs and Their Potential Target Genes in Adipose Tissue from Pigs with Highly Divergent Backfat Thickness |
| title_full_unstemmed | Identification of Differentially Expressed MicroRNAs and Their Potential Target Genes in Adipose Tissue from Pigs with Highly Divergent Backfat Thickness |
| title_short | Identification of Differentially Expressed MicroRNAs and Their Potential Target Genes in Adipose Tissue from Pigs with Highly Divergent Backfat Thickness |
| title_sort | identification of differentially expressed micrornas and their potential target genes in adipose tissue from pigs with highly divergent backfat thickness |
| topic | pig miRNA fat deposition regulatory network |
| url | https://www.mdpi.com/2076-2615/10/4/624 |
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