A new oligonucleotide microarray for detection of pathogenic and non-pathogenic Legionella spp.
Legionella pneumophila has been recognized as the major cause of legionellosis since the discovery of the deadly disease. Legionella spp. other than L. pneumophila were later found to be responsible to many non-pneumophila infections. The non-L. pneumophila infections are likely under-detected becau...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4254607?pdf=render |
| _version_ | 1818409422284652544 |
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| author | Boyang Cao Xiangqian Liu Xiang Yu Min Chen Lu Feng Lei Wang |
| author_facet | Boyang Cao Xiangqian Liu Xiang Yu Min Chen Lu Feng Lei Wang |
| author_sort | Boyang Cao |
| collection | DOAJ |
| description | Legionella pneumophila has been recognized as the major cause of legionellosis since the discovery of the deadly disease. Legionella spp. other than L. pneumophila were later found to be responsible to many non-pneumophila infections. The non-L. pneumophila infections are likely under-detected because of a lack of effective diagnosis. In this report, we have sequenced the 16S-23S rRNA gene internal transcribed spacer (ITS) of 10 Legionella species and subspecies, including L. anisa, L. bozemanii, L. dumoffii, L. fairfieldensis, L. gormanii, L. jordanis, L. maceachernii, L. micdadei, L. pneumophila subspp. fraseri and L. pneumophila subspp. pasculleii, and developed a rapid oligonucleotide microarray detection technique accordingly to identify 12 most common Legionella spp., which consist of 11 pathogenic species of L. anisa, L. bozemanii, L. dumoffii, L. gormanii, L. jordanis, L. longbeachae, L. maceachernii, L. micdadei, and L. pneumophila (including subspp. pneumophila, subspp. fraseri, and subspp. pasculleii) and one non-pathogenic species, L. fairfieldensis. Twenty-nine probes that reproducibly detected multiple Legionella species with high specificity were included in the array. A total of 52 strains, including 30 target pathogens and 22 non-target bacteria, were used to verify the oligonucleotide microarray assay. The sensitivity of the detection was at 1.0 ng with genomic DNA or 13 CFU/100 mL with Legionella cultures. The microarray detected seven samples of air conditioner-condensed water with 100% accuracy, validating the technique as a promising method for applications in basic microbiology, clinical diagnosis, food safety, and epidemiological surveillance. The phylogenetic study based on the ITS has also revealed that the non-pathogenic L. fairfieldensis is the closest to L. pneumophila than the nine other pathogenic Legionella spp. |
| first_indexed | 2024-12-14T09:59:22Z |
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| id | doaj.art-53d3b1af4e864ebfbd2cd68ddc7d5faa |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-14T09:59:22Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-53d3b1af4e864ebfbd2cd68ddc7d5faa2022-12-21T23:07:18ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-01912e11386310.1371/journal.pone.0113863A new oligonucleotide microarray for detection of pathogenic and non-pathogenic Legionella spp.Boyang CaoXiangqian LiuXiang YuMin ChenLu FengLei WangLegionella pneumophila has been recognized as the major cause of legionellosis since the discovery of the deadly disease. Legionella spp. other than L. pneumophila were later found to be responsible to many non-pneumophila infections. The non-L. pneumophila infections are likely under-detected because of a lack of effective diagnosis. In this report, we have sequenced the 16S-23S rRNA gene internal transcribed spacer (ITS) of 10 Legionella species and subspecies, including L. anisa, L. bozemanii, L. dumoffii, L. fairfieldensis, L. gormanii, L. jordanis, L. maceachernii, L. micdadei, L. pneumophila subspp. fraseri and L. pneumophila subspp. pasculleii, and developed a rapid oligonucleotide microarray detection technique accordingly to identify 12 most common Legionella spp., which consist of 11 pathogenic species of L. anisa, L. bozemanii, L. dumoffii, L. gormanii, L. jordanis, L. longbeachae, L. maceachernii, L. micdadei, and L. pneumophila (including subspp. pneumophila, subspp. fraseri, and subspp. pasculleii) and one non-pathogenic species, L. fairfieldensis. Twenty-nine probes that reproducibly detected multiple Legionella species with high specificity were included in the array. A total of 52 strains, including 30 target pathogens and 22 non-target bacteria, were used to verify the oligonucleotide microarray assay. The sensitivity of the detection was at 1.0 ng with genomic DNA or 13 CFU/100 mL with Legionella cultures. The microarray detected seven samples of air conditioner-condensed water with 100% accuracy, validating the technique as a promising method for applications in basic microbiology, clinical diagnosis, food safety, and epidemiological surveillance. The phylogenetic study based on the ITS has also revealed that the non-pathogenic L. fairfieldensis is the closest to L. pneumophila than the nine other pathogenic Legionella spp.http://europepmc.org/articles/PMC4254607?pdf=render |
| spellingShingle | Boyang Cao Xiangqian Liu Xiang Yu Min Chen Lu Feng Lei Wang A new oligonucleotide microarray for detection of pathogenic and non-pathogenic Legionella spp. PLoS ONE |
| title | A new oligonucleotide microarray for detection of pathogenic and non-pathogenic Legionella spp. |
| title_full | A new oligonucleotide microarray for detection of pathogenic and non-pathogenic Legionella spp. |
| title_fullStr | A new oligonucleotide microarray for detection of pathogenic and non-pathogenic Legionella spp. |
| title_full_unstemmed | A new oligonucleotide microarray for detection of pathogenic and non-pathogenic Legionella spp. |
| title_short | A new oligonucleotide microarray for detection of pathogenic and non-pathogenic Legionella spp. |
| title_sort | new oligonucleotide microarray for detection of pathogenic and non pathogenic legionella spp |
| url | http://europepmc.org/articles/PMC4254607?pdf=render |
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