Quantification of Pseudomonas aeruginosa in multispecies biofilms using PMA-qPCR
Multispecies biofilms are an important healthcare problem and may lead to persistent infections. These infections are difficult to treat, as cells in a biofilm are highly resistant to antimicrobial agents. While increasingly being recognized as important, the properties of multispecies biofilms rema...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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PeerJ Inc.
2015-02-01
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| Series: | PeerJ |
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| Online Access: | https://peerj.com/articles/787.pdf |
| _version_ | 1827607399538622464 |
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| author | Sarah Tavernier Tom Coenye |
| author_facet | Sarah Tavernier Tom Coenye |
| author_sort | Sarah Tavernier |
| collection | DOAJ |
| description | Multispecies biofilms are an important healthcare problem and may lead to persistent infections. These infections are difficult to treat, as cells in a biofilm are highly resistant to antimicrobial agents. While increasingly being recognized as important, the properties of multispecies biofilms remain poorly studied. In order to do so, the quantification of the individual species is needed. The current cultivation-based approaches can lead to an underestimation of the actual cell number and are time-consuming. In the present study we set up a culture-independent approach based on propidium monoazide qPCR (PMA-qPCR) to quantify Pseudomonas aeruginosa in a multispecies biofilm. As a proof of concept, we explored the influence of the combined presence of Staphylococcus aureus, Streptococcus anginosus and Burkholderia cenocepacia on the antimicrobial susceptibility of P. aeruginosa using this PMA-qPCR approach. |
| first_indexed | 2024-03-09T06:53:05Z |
| format | Article |
| id | doaj.art-53d4e83cbd2e4276994a1b1d2b0c846a |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T06:53:05Z |
| publishDate | 2015-02-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
| series | PeerJ |
| spelling | doaj.art-53d4e83cbd2e4276994a1b1d2b0c846a2023-12-03T10:14:41ZengPeerJ Inc.PeerJ2167-83592015-02-013e78710.7717/peerj.787787Quantification of Pseudomonas aeruginosa in multispecies biofilms using PMA-qPCRSarah Tavernier0Tom Coenye1Laboratory of Pharmaceutical Microbiology, Ghent University, Ghent, BelgiumLaboratory of Pharmaceutical Microbiology, Ghent University, Ghent, BelgiumMultispecies biofilms are an important healthcare problem and may lead to persistent infections. These infections are difficult to treat, as cells in a biofilm are highly resistant to antimicrobial agents. While increasingly being recognized as important, the properties of multispecies biofilms remain poorly studied. In order to do so, the quantification of the individual species is needed. The current cultivation-based approaches can lead to an underestimation of the actual cell number and are time-consuming. In the present study we set up a culture-independent approach based on propidium monoazide qPCR (PMA-qPCR) to quantify Pseudomonas aeruginosa in a multispecies biofilm. As a proof of concept, we explored the influence of the combined presence of Staphylococcus aureus, Streptococcus anginosus and Burkholderia cenocepacia on the antimicrobial susceptibility of P. aeruginosa using this PMA-qPCR approach.https://peerj.com/articles/787.pdfBiofilm Pseudomonas aeruginosa ResistancePMA-qPCR |
| spellingShingle | Sarah Tavernier Tom Coenye Quantification of Pseudomonas aeruginosa in multispecies biofilms using PMA-qPCR PeerJ Biofilm Pseudomonas aeruginosa Resistance PMA-qPCR |
| title | Quantification of Pseudomonas aeruginosa in multispecies biofilms using PMA-qPCR |
| title_full | Quantification of Pseudomonas aeruginosa in multispecies biofilms using PMA-qPCR |
| title_fullStr | Quantification of Pseudomonas aeruginosa in multispecies biofilms using PMA-qPCR |
| title_full_unstemmed | Quantification of Pseudomonas aeruginosa in multispecies biofilms using PMA-qPCR |
| title_short | Quantification of Pseudomonas aeruginosa in multispecies biofilms using PMA-qPCR |
| title_sort | quantification of pseudomonas aeruginosa in multispecies biofilms using pma qpcr |
| topic | Biofilm Pseudomonas aeruginosa Resistance PMA-qPCR |
| url | https://peerj.com/articles/787.pdf |
| work_keys_str_mv | AT sarahtavernier quantificationofpseudomonasaeruginosainmultispeciesbiofilmsusingpmaqpcr AT tomcoenye quantificationofpseudomonasaeruginosainmultispeciesbiofilmsusingpmaqpcr |