Tendinosis-like changes in denervated rat Achilles tendon
Abstract Background Tendon disorders are common and lead to significant disability and pain. Our knowledge of the ‘tennis elbow’, the ‘jumpers knee’, and Achilles tendinosis has increased over the years, but changes in denervated tendons is yet to be described in detail. The aim of the present study...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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BMC
2018-11-01
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| Series: | BMC Musculoskeletal Disorders |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12891-018-2353-7 |
| _version_ | 1818837523318702080 |
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| author | Roine El-Habta Jialin Chen Jessica Pingel Ludvig J. Backman |
| author_facet | Roine El-Habta Jialin Chen Jessica Pingel Ludvig J. Backman |
| author_sort | Roine El-Habta |
| collection | DOAJ |
| description | Abstract Background Tendon disorders are common and lead to significant disability and pain. Our knowledge of the ‘tennis elbow’, the ‘jumpers knee’, and Achilles tendinosis has increased over the years, but changes in denervated tendons is yet to be described in detail. The aim of the present study was to investigate the morphological and biochemical changes in tendon tissue following two weeks of denervation using a unilateral sciatic nerve transection model in rat Achilles tendons. Methods Tendons were compared with respect to cell number, nuclear roundness, and fiber structure. The non-denervated contralateral tendon served as a control. Also, the expression of neuromodulators such as substance P and its preferred receptor neurokinin-1 receptor, NK-1R, was evaluated using real-time qRT-PCR. Results Our results showed that denervated tendons expressed morphological changes such as hypercellularity; disfigured cells; disorganization of the collagen network; increased production of type III collagen; and increased expression of NK-1R. Conclusion Taken together these data provide new insights into the histopathology of denervated tendons showing that denervation causes somewhat similar changes in the Achilles tendon as does tendinosis in rats. |
| first_indexed | 2024-12-19T03:23:51Z |
| format | Article |
| id | doaj.art-53d4fd6eb16f4876b00de932d3898740 |
| institution | Directory Open Access Journal |
| issn | 1471-2474 |
| language | English |
| last_indexed | 2024-12-19T03:23:51Z |
| publishDate | 2018-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Musculoskeletal Disorders |
| spelling | doaj.art-53d4fd6eb16f4876b00de932d38987402022-12-21T20:37:42ZengBMCBMC Musculoskeletal Disorders1471-24742018-11-011911910.1186/s12891-018-2353-7Tendinosis-like changes in denervated rat Achilles tendonRoine El-Habta0Jialin Chen1Jessica Pingel2Ludvig J. Backman3Department of Integrative Medical Biology, Section for Anatomy, Umeå UniversityDepartment of Integrative Medical Biology, Section for Anatomy, Umeå UniversityDepartment of Neuroscience, University of CopenhagenDepartment of Integrative Medical Biology, Section for Anatomy, Umeå UniversityAbstract Background Tendon disorders are common and lead to significant disability and pain. Our knowledge of the ‘tennis elbow’, the ‘jumpers knee’, and Achilles tendinosis has increased over the years, but changes in denervated tendons is yet to be described in detail. The aim of the present study was to investigate the morphological and biochemical changes in tendon tissue following two weeks of denervation using a unilateral sciatic nerve transection model in rat Achilles tendons. Methods Tendons were compared with respect to cell number, nuclear roundness, and fiber structure. The non-denervated contralateral tendon served as a control. Also, the expression of neuromodulators such as substance P and its preferred receptor neurokinin-1 receptor, NK-1R, was evaluated using real-time qRT-PCR. Results Our results showed that denervated tendons expressed morphological changes such as hypercellularity; disfigured cells; disorganization of the collagen network; increased production of type III collagen; and increased expression of NK-1R. Conclusion Taken together these data provide new insights into the histopathology of denervated tendons showing that denervation causes somewhat similar changes in the Achilles tendon as does tendinosis in rats.http://link.springer.com/article/10.1186/s12891-018-2353-7CollagenDenervationRatSubstance PTendinosis |
| spellingShingle | Roine El-Habta Jialin Chen Jessica Pingel Ludvig J. Backman Tendinosis-like changes in denervated rat Achilles tendon BMC Musculoskeletal Disorders Collagen Denervation Rat Substance P Tendinosis |
| title | Tendinosis-like changes in denervated rat Achilles tendon |
| title_full | Tendinosis-like changes in denervated rat Achilles tendon |
| title_fullStr | Tendinosis-like changes in denervated rat Achilles tendon |
| title_full_unstemmed | Tendinosis-like changes in denervated rat Achilles tendon |
| title_short | Tendinosis-like changes in denervated rat Achilles tendon |
| title_sort | tendinosis like changes in denervated rat achilles tendon |
| topic | Collagen Denervation Rat Substance P Tendinosis |
| url | http://link.springer.com/article/10.1186/s12891-018-2353-7 |
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