Genetic variability in different growth forms of Dendrocalamus strictus: Deogun revisited
Abstract Background Bamboo is among the important plants that help shape the socio-economic fabric of rural India. It provides employment, sustains business ventures, has medicinal applications and even helps in carbon sequestration. Out of 125 indigenous species, Dendrocalamus strictus (Roxb.) Nees...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Scion
2017-11-01
|
| Series: | New Zealand Journal of Forestry Science |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s40490-017-0104-4 |
| _version_ | 1818936118834364416 |
|---|---|
| author | Solomon Das Y. P. Singh Yogesh K. Negi P. C. Shrivastav |
| author_facet | Solomon Das Y. P. Singh Yogesh K. Negi P. C. Shrivastav |
| author_sort | Solomon Das |
| collection | DOAJ |
| description | Abstract Background Bamboo is among the important plants that help shape the socio-economic fabric of rural India. It provides employment, sustains business ventures, has medicinal applications and even helps in carbon sequestration. Out of 125 indigenous species, Dendrocalamus strictus (Roxb.) Nees occupies 53% of the area of bamboo in the country. Moreover, D. strictus may be used in afforestation of wastelands and rural development programmes due to its adaptability in wider landscapes. Dendrocalamus strictus has different growth forms based on edaphic factors and climatic conditions. DNA profiling was used to analyse the genetic diversity among the different growth forms of D. strictus present in three different locations of Uttarakhand. Methods The study area includes three locations, first, reserve forest of the Forest Research Institute (FRI), Dehradun; second, Shivpuri near Byasi, Rishikesh; and third, Chiriapur range (Haridwar district). A standard method was used to isolate DNA from young leaves from ten clumps of each growth form. Ten RAPD primers were screened for polymorphism from A and N operon primers and a standard PCR protocol was followed to amplify and visualise DNA bands. The data matrix was analysed and interpreted using statistical software and methods. Results The cluster analysis, genetic structure parameters, moderate coefficient of gene differentiation and low gene flow value all indicated that these growth forms are genetically dissimilar and that geographic separation as well as physiological/flowering barriers has influenced these variations. These genetically different growth forms can be called ecotypes. Conclusions Such a study has not been attempted previously with bamboo and will help inform the conservation of the genetic pool of bamboo ecotypes. Seeds of these ecotypes are monocarpic in nature, which means that bamboo plants flower once in their lifetime, so they must be collected and multiplied (as plantations) in their respective habitats. |
| first_indexed | 2024-12-20T05:30:59Z |
| format | Article |
| id | doaj.art-53d6376e31e141c0929b5f03fc60a404 |
| institution | Directory Open Access Journal |
| issn | 1179-5395 |
| language | English |
| last_indexed | 2024-12-20T05:30:59Z |
| publishDate | 2017-11-01 |
| publisher | Scion |
| record_format | Article |
| series | New Zealand Journal of Forestry Science |
| spelling | doaj.art-53d6376e31e141c0929b5f03fc60a4042022-12-21T19:51:44ZengScionNew Zealand Journal of Forestry Science1179-53952017-11-0147111210.1186/s40490-017-0104-4Genetic variability in different growth forms of Dendrocalamus strictus: Deogun revisitedSolomon Das0Y. P. Singh1Yogesh K. Negi2P. C. Shrivastav3Vimix Solutions Private LimitedDepartment of Forest Pathology, Forest Research InstituteCollege of Forestry, Uttarakhand University of Horticulture and ForestryDepartment of Soil Science, College of Agriculture, G. B. Pant University of Agriculture and TechnologyAbstract Background Bamboo is among the important plants that help shape the socio-economic fabric of rural India. It provides employment, sustains business ventures, has medicinal applications and even helps in carbon sequestration. Out of 125 indigenous species, Dendrocalamus strictus (Roxb.) Nees occupies 53% of the area of bamboo in the country. Moreover, D. strictus may be used in afforestation of wastelands and rural development programmes due to its adaptability in wider landscapes. Dendrocalamus strictus has different growth forms based on edaphic factors and climatic conditions. DNA profiling was used to analyse the genetic diversity among the different growth forms of D. strictus present in three different locations of Uttarakhand. Methods The study area includes three locations, first, reserve forest of the Forest Research Institute (FRI), Dehradun; second, Shivpuri near Byasi, Rishikesh; and third, Chiriapur range (Haridwar district). A standard method was used to isolate DNA from young leaves from ten clumps of each growth form. Ten RAPD primers were screened for polymorphism from A and N operon primers and a standard PCR protocol was followed to amplify and visualise DNA bands. The data matrix was analysed and interpreted using statistical software and methods. Results The cluster analysis, genetic structure parameters, moderate coefficient of gene differentiation and low gene flow value all indicated that these growth forms are genetically dissimilar and that geographic separation as well as physiological/flowering barriers has influenced these variations. These genetically different growth forms can be called ecotypes. Conclusions Such a study has not been attempted previously with bamboo and will help inform the conservation of the genetic pool of bamboo ecotypes. Seeds of these ecotypes are monocarpic in nature, which means that bamboo plants flower once in their lifetime, so they must be collected and multiplied (as plantations) in their respective habitats.http://link.springer.com/article/10.1186/s40490-017-0104-4BambooEcotypesRAPDVariability |
| spellingShingle | Solomon Das Y. P. Singh Yogesh K. Negi P. C. Shrivastav Genetic variability in different growth forms of Dendrocalamus strictus: Deogun revisited New Zealand Journal of Forestry Science Bamboo Ecotypes RAPD Variability |
| title | Genetic variability in different growth forms of Dendrocalamus strictus: Deogun revisited |
| title_full | Genetic variability in different growth forms of Dendrocalamus strictus: Deogun revisited |
| title_fullStr | Genetic variability in different growth forms of Dendrocalamus strictus: Deogun revisited |
| title_full_unstemmed | Genetic variability in different growth forms of Dendrocalamus strictus: Deogun revisited |
| title_short | Genetic variability in different growth forms of Dendrocalamus strictus: Deogun revisited |
| title_sort | genetic variability in different growth forms of dendrocalamus strictus deogun revisited |
| topic | Bamboo Ecotypes RAPD Variability |
| url | http://link.springer.com/article/10.1186/s40490-017-0104-4 |
| work_keys_str_mv | AT solomondas geneticvariabilityindifferentgrowthformsofdendrocalamusstrictusdeogunrevisited AT ypsingh geneticvariabilityindifferentgrowthformsofdendrocalamusstrictusdeogunrevisited AT yogeshknegi geneticvariabilityindifferentgrowthformsofdendrocalamusstrictusdeogunrevisited AT pcshrivastav geneticvariabilityindifferentgrowthformsofdendrocalamusstrictusdeogunrevisited |