Multi-walled carbon nanotubes-induced alterations in microRNA let-7 and its targets activate a protection mechanism by conferring a developmental timing control
Abstract Background Multi-walled carbon nanotubes (MWCNTs) have been produced and applied for diverse purposes. MWCNTs can potentially cause the adverse effects on organisms. MicroRNA let-7 and its targets of HBL-1 and LIN-41 play a central role in regulating developmental timing by acting as a deve...
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| Format: | Article |
| Language: | English |
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BMC
2017-07-01
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| Series: | Particle and Fibre Toxicology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12989-017-0208-2 |
| _version_ | 1818133954887155712 |
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| author | Li Zhao Hanxiao Wan Qizhan Liu Dayong Wang |
| author_facet | Li Zhao Hanxiao Wan Qizhan Liu Dayong Wang |
| author_sort | Li Zhao |
| collection | DOAJ |
| description | Abstract Background Multi-walled carbon nanotubes (MWCNTs) have been produced and applied for diverse purposes. MWCNTs can potentially cause the adverse effects on organisms. MicroRNA let-7 and its targets of HBL-1 and LIN-41 play a central role in regulating developmental timing by acting as a developmental switch. The sequence of let-7 and the underlying mechanisms for let-7 in the control of developmental timing are conserved among different species. In this study, we investigated the potential effect of MWCNTs exposure on the molecular basis for developmental timing mediated by let-7 and its targets of HBL-1 and LIN-41. Results MWCNTs exposure decreased let-7 expression, and increased expressions of hbl-1 and lin-41. let-7 mutant was resistant to MWCNTs toxicity, whereas hbl-1 or lin-41 mutant was susceptible to MWCNTs toxicity. hbl-1 or lin-41 mutant suppressed the resistance of let-7 mutant to MWCNTs toxicity. HBL-1 acted upstream of TIR-1, an adaptor protein, and SYM-1, a protein containing leucine-rich repeats, and ALG-1 and ALG-2, two RDE-1 proteins related to RNA interference (RNAi) pathway, acted upstream of LIN-41 to regulate MWCNTs toxicity. Moreover, we identified a feedback loop between let-7 and its targets of HBL-1 and LIN-41 in the regulation of MWCNTs toxicity. The observed increase in let-7::GFP expression in MWCNTs exposed nematodes with mutation of hbl-1 or lin-41 implied that a feedback mechanism may exist to strengthen the function of let-7 suppression in protecting the animals from MWCNTs toxicity. Conclusions Our results demonstrate the protection function of let-7 suppression for animals from MWCNTs toxicity by conferring a robust developmental timing control. Our results highlight the importance of molecular basis for developmental timing in influencing MWCNTs toxicity. |
| first_indexed | 2024-12-11T09:00:56Z |
| format | Article |
| id | doaj.art-53d6e3e00a4040d4b52b77aa22d03f8e |
| institution | Directory Open Access Journal |
| issn | 1743-8977 |
| language | English |
| last_indexed | 2024-12-11T09:00:56Z |
| publishDate | 2017-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Particle and Fibre Toxicology |
| spelling | doaj.art-53d6e3e00a4040d4b52b77aa22d03f8e2022-12-22T01:13:46ZengBMCParticle and Fibre Toxicology1743-89772017-07-0114111110.1186/s12989-017-0208-2Multi-walled carbon nanotubes-induced alterations in microRNA let-7 and its targets activate a protection mechanism by conferring a developmental timing controlLi Zhao0Hanxiao Wan1Qizhan Liu2Dayong Wang3Key Laboratory of Environmental Medicine Engineering in Ministry of Education, Medical School, Southeast UniversityKey Laboratory of Environmental Medicine Engineering in Ministry of Education, Medical School, Southeast UniversitySchool of Public Health, Nanjing Medical UniversityKey Laboratory of Environmental Medicine Engineering in Ministry of Education, Medical School, Southeast UniversityAbstract Background Multi-walled carbon nanotubes (MWCNTs) have been produced and applied for diverse purposes. MWCNTs can potentially cause the adverse effects on organisms. MicroRNA let-7 and its targets of HBL-1 and LIN-41 play a central role in regulating developmental timing by acting as a developmental switch. The sequence of let-7 and the underlying mechanisms for let-7 in the control of developmental timing are conserved among different species. In this study, we investigated the potential effect of MWCNTs exposure on the molecular basis for developmental timing mediated by let-7 and its targets of HBL-1 and LIN-41. Results MWCNTs exposure decreased let-7 expression, and increased expressions of hbl-1 and lin-41. let-7 mutant was resistant to MWCNTs toxicity, whereas hbl-1 or lin-41 mutant was susceptible to MWCNTs toxicity. hbl-1 or lin-41 mutant suppressed the resistance of let-7 mutant to MWCNTs toxicity. HBL-1 acted upstream of TIR-1, an adaptor protein, and SYM-1, a protein containing leucine-rich repeats, and ALG-1 and ALG-2, two RDE-1 proteins related to RNA interference (RNAi) pathway, acted upstream of LIN-41 to regulate MWCNTs toxicity. Moreover, we identified a feedback loop between let-7 and its targets of HBL-1 and LIN-41 in the regulation of MWCNTs toxicity. The observed increase in let-7::GFP expression in MWCNTs exposed nematodes with mutation of hbl-1 or lin-41 implied that a feedback mechanism may exist to strengthen the function of let-7 suppression in protecting the animals from MWCNTs toxicity. Conclusions Our results demonstrate the protection function of let-7 suppression for animals from MWCNTs toxicity by conferring a robust developmental timing control. Our results highlight the importance of molecular basis for developmental timing in influencing MWCNTs toxicity.http://link.springer.com/article/10.1186/s12989-017-0208-2let-7Multi-walled carbon nanotubesDevelopmental timingNanotoxicityCaenorhabditis elegans |
| spellingShingle | Li Zhao Hanxiao Wan Qizhan Liu Dayong Wang Multi-walled carbon nanotubes-induced alterations in microRNA let-7 and its targets activate a protection mechanism by conferring a developmental timing control Particle and Fibre Toxicology let-7 Multi-walled carbon nanotubes Developmental timing Nanotoxicity Caenorhabditis elegans |
| title | Multi-walled carbon nanotubes-induced alterations in microRNA let-7 and its targets activate a protection mechanism by conferring a developmental timing control |
| title_full | Multi-walled carbon nanotubes-induced alterations in microRNA let-7 and its targets activate a protection mechanism by conferring a developmental timing control |
| title_fullStr | Multi-walled carbon nanotubes-induced alterations in microRNA let-7 and its targets activate a protection mechanism by conferring a developmental timing control |
| title_full_unstemmed | Multi-walled carbon nanotubes-induced alterations in microRNA let-7 and its targets activate a protection mechanism by conferring a developmental timing control |
| title_short | Multi-walled carbon nanotubes-induced alterations in microRNA let-7 and its targets activate a protection mechanism by conferring a developmental timing control |
| title_sort | multi walled carbon nanotubes induced alterations in microrna let 7 and its targets activate a protection mechanism by conferring a developmental timing control |
| topic | let-7 Multi-walled carbon nanotubes Developmental timing Nanotoxicity Caenorhabditis elegans |
| url | http://link.springer.com/article/10.1186/s12989-017-0208-2 |
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