<i>Rhizocarpon geographicum</i> Lichen Discloses a Highly Diversified Microbiota Carrying Antibiotic Resistance and Persistent Organic Pollutant Tolerance
As rock inhabitants, lichens are exposed to extreme and fluctuating abiotic conditions associated with poor sources of nutriments. These extreme conditions confer to lichens the unique ability to develop protective mechanisms. Consequently, lichen-associated microbes disclose highly versatile lifest...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-09-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/9/1859 |
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| author | Alice Miral Adam Kautsky Susete Alves-Carvalho Ludovic Cottret Anne-Yvonne Guillerm-Erckelboudt Manon Buguet Isabelle Rouaud Sylvain Tranchimand Sophie Tomasi Claudia Bartoli |
| author_facet | Alice Miral Adam Kautsky Susete Alves-Carvalho Ludovic Cottret Anne-Yvonne Guillerm-Erckelboudt Manon Buguet Isabelle Rouaud Sylvain Tranchimand Sophie Tomasi Claudia Bartoli |
| author_sort | Alice Miral |
| collection | DOAJ |
| description | As rock inhabitants, lichens are exposed to extreme and fluctuating abiotic conditions associated with poor sources of nutriments. These extreme conditions confer to lichens the unique ability to develop protective mechanisms. Consequently, lichen-associated microbes disclose highly versatile lifestyles and ecological plasticity, enabling them to withstand extreme environments. Because of their ability to grow in poor and extreme habitats, bacteria associated with lichens can tolerate a wide range of pollutants, and they are known to produce antimicrobial compounds. In addition, lichen-associated bacteria have been described to harbor ecological functions crucial for the evolution of the lichen holobiont. Nevertheless, the ecological features of lichen-associated microbes are still underestimated. To explore the untapped ecological diversity of lichen-associated bacteria, we adopted a novel culturomic approach on the crustose lichen <i>Rhizocarpon geographicum</i>. We sampled <i>R. geographicum</i> in French habitats exposed to oil spills, and we combined nine culturing methods with 16S rRNA sequencing to capture the greatest bacterial diversity. A deep functional analysis of the lichen-associated bacterial collection showed the presence of a set of bacterial strains resistant to a wide range of antibiotics and displaying tolerance to Persistent Organic Pollutants (POPs). Our study is a starting point to explore the ecological features of the lichen microbiota. |
| first_indexed | 2024-03-09T23:04:51Z |
| format | Article |
| id | doaj.art-53d815db8354496eba368e04c6ae9818 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T23:04:51Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-53d815db8354496eba368e04c6ae98182023-11-23T17:54:27ZengMDPI AGMicroorganisms2076-26072022-09-01109185910.3390/microorganisms10091859<i>Rhizocarpon geographicum</i> Lichen Discloses a Highly Diversified Microbiota Carrying Antibiotic Resistance and Persistent Organic Pollutant ToleranceAlice Miral0Adam Kautsky1Susete Alves-Carvalho2Ludovic Cottret3Anne-Yvonne Guillerm-Erckelboudt4Manon Buguet5Isabelle Rouaud6Sylvain Tranchimand7Sophie Tomasi8Claudia Bartoli9CNRS, ISCR (Institut des Sciences Chimiques de Rennes)—UMR 6226, University of Rennes 1, 35000 Rennes, FranceIGEPP, INRAE, Institut Agro, University of Rennes 1, LIPME, INRAE, 35653 Le Rheu, FranceIGEPP, INRAE, Institut Agro, University of Rennes 1, LIPME, INRAE, 35653 Le Rheu, FranceCNRS, Université de Toulouse, 31320 Castanet-Tolosan, FranceIGEPP, INRAE, Institut Agro, University of Rennes 1, LIPME, INRAE, 35653 Le Rheu, FranceCNRS, ISCR (Institut des Sciences Chimiques de Rennes)—UMR 6226, University of Rennes 1, 35000 Rennes, FranceCNRS, ISCR (Institut des Sciences Chimiques de Rennes)—UMR 6226, University of Rennes 1, 35000 Rennes, FranceEcole Nationale Supérieure de Chimie de Rennes, CNRS, ISCR (Institut des Sciences Chimiques de Rennes)—UMR 6226, University of Rennes 1, 35000 Rennes, FranceCNRS, ISCR (Institut des Sciences Chimiques de Rennes)—UMR 6226, University of Rennes 1, 35000 Rennes, FranceIGEPP, INRAE, Institut Agro, University of Rennes 1, LIPME, INRAE, 35653 Le Rheu, FranceAs rock inhabitants, lichens are exposed to extreme and fluctuating abiotic conditions associated with poor sources of nutriments. These extreme conditions confer to lichens the unique ability to develop protective mechanisms. Consequently, lichen-associated microbes disclose highly versatile lifestyles and ecological plasticity, enabling them to withstand extreme environments. Because of their ability to grow in poor and extreme habitats, bacteria associated with lichens can tolerate a wide range of pollutants, and they are known to produce antimicrobial compounds. In addition, lichen-associated bacteria have been described to harbor ecological functions crucial for the evolution of the lichen holobiont. Nevertheless, the ecological features of lichen-associated microbes are still underestimated. To explore the untapped ecological diversity of lichen-associated bacteria, we adopted a novel culturomic approach on the crustose lichen <i>Rhizocarpon geographicum</i>. We sampled <i>R. geographicum</i> in French habitats exposed to oil spills, and we combined nine culturing methods with 16S rRNA sequencing to capture the greatest bacterial diversity. A deep functional analysis of the lichen-associated bacterial collection showed the presence of a set of bacterial strains resistant to a wide range of antibiotics and displaying tolerance to Persistent Organic Pollutants (POPs). Our study is a starting point to explore the ecological features of the lichen microbiota.https://www.mdpi.com/2076-2607/10/9/1859culturomicsholobiontantibiotic resistancelichen microbiome |
| spellingShingle | Alice Miral Adam Kautsky Susete Alves-Carvalho Ludovic Cottret Anne-Yvonne Guillerm-Erckelboudt Manon Buguet Isabelle Rouaud Sylvain Tranchimand Sophie Tomasi Claudia Bartoli <i>Rhizocarpon geographicum</i> Lichen Discloses a Highly Diversified Microbiota Carrying Antibiotic Resistance and Persistent Organic Pollutant Tolerance Microorganisms culturomics holobiont antibiotic resistance lichen microbiome |
| title | <i>Rhizocarpon geographicum</i> Lichen Discloses a Highly Diversified Microbiota Carrying Antibiotic Resistance and Persistent Organic Pollutant Tolerance |
| title_full | <i>Rhizocarpon geographicum</i> Lichen Discloses a Highly Diversified Microbiota Carrying Antibiotic Resistance and Persistent Organic Pollutant Tolerance |
| title_fullStr | <i>Rhizocarpon geographicum</i> Lichen Discloses a Highly Diversified Microbiota Carrying Antibiotic Resistance and Persistent Organic Pollutant Tolerance |
| title_full_unstemmed | <i>Rhizocarpon geographicum</i> Lichen Discloses a Highly Diversified Microbiota Carrying Antibiotic Resistance and Persistent Organic Pollutant Tolerance |
| title_short | <i>Rhizocarpon geographicum</i> Lichen Discloses a Highly Diversified Microbiota Carrying Antibiotic Resistance and Persistent Organic Pollutant Tolerance |
| title_sort | i rhizocarpon geographicum i lichen discloses a highly diversified microbiota carrying antibiotic resistance and persistent organic pollutant tolerance |
| topic | culturomics holobiont antibiotic resistance lichen microbiome |
| url | https://www.mdpi.com/2076-2607/10/9/1859 |
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