Anti-inflammatory effects of bamboo salt and sodium fluoride in human gingival fibroblasts–An in vitro study
Dental caries preventive agents, such as sodium fluoride (NaF) and bamboo salt (BS), are known to cause cellular growth that is characterized by morphological and gene expression changes. This study was designed to investigate the dual effect of NaF and BS on interleukin (IL)-1β-induced gingival inf...
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| Format: | Article |
| Language: | English |
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Wiley
2015-06-01
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| Series: | Kaohsiung Journal of Medical Sciences |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1607551X15000935 |
| _version_ | 1818521261230260224 |
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| author | Hye-Jin Lee Choong-Ho Choi |
| author_facet | Hye-Jin Lee Choong-Ho Choi |
| author_sort | Hye-Jin Lee |
| collection | DOAJ |
| description | Dental caries preventive agents, such as sodium fluoride (NaF) and bamboo salt (BS), are known to cause cellular growth that is characterized by morphological and gene expression changes. This study was designed to investigate the dual effect of NaF and BS on interleukin (IL)-1β-induced gingival inflammation. Under in vitro experimental conditions, exposure to a subcytotoxic dose of IL-1β enhanced human gingival fibroblast inflammation, as characterized by increased levels of inflammation-associated proteins. A combination of NaF and BS significantly protected fibroblasts from IL-1β-induced cellular deterioration. Exposure to NaF and BS induced the cell growth and no changes in viability were found with the Lactate Dehydrogenase Assay (LDH) assay at the NaF and BS concentration analyzed. Molecular analysis demonstrated that NaF and BS increased resistance to inflammation by reduction of IL-1β, IL-8, and tumor necrosis factor (TNF)-α production. In addition, NaF and BS decreased the expression of IL-1β, IL-8, and TNF-α mRNA in IL-1β-induced human gingival fibroblast cells. The study identifies a new role for NaF and BS in the IL-1β-induced inflammation of gingival fibroblasts and provides a potential target for gingival protection. |
| first_indexed | 2024-12-11T01:48:41Z |
| format | Article |
| id | doaj.art-53da6c20efb140abb26554605848a870 |
| institution | Directory Open Access Journal |
| issn | 1607-551X |
| language | English |
| last_indexed | 2024-12-11T01:48:41Z |
| publishDate | 2015-06-01 |
| publisher | Wiley |
| record_format | Article |
| series | Kaohsiung Journal of Medical Sciences |
| spelling | doaj.art-53da6c20efb140abb26554605848a8702022-12-22T01:24:49ZengWileyKaohsiung Journal of Medical Sciences1607-551X2015-06-0131630330810.1016/j.kjms.2015.03.005Anti-inflammatory effects of bamboo salt and sodium fluoride in human gingival fibroblasts–An in vitro studyHye-Jin Lee0Choong-Ho Choi1Department of Dental Hygiene, Howon University, Gunsan, Republic of KoreaDepartment of Preventive and Public Health Dentistry, Chonnam National University School of Dentistry, Gwangju, Republic of KoreaDental caries preventive agents, such as sodium fluoride (NaF) and bamboo salt (BS), are known to cause cellular growth that is characterized by morphological and gene expression changes. This study was designed to investigate the dual effect of NaF and BS on interleukin (IL)-1β-induced gingival inflammation. Under in vitro experimental conditions, exposure to a subcytotoxic dose of IL-1β enhanced human gingival fibroblast inflammation, as characterized by increased levels of inflammation-associated proteins. A combination of NaF and BS significantly protected fibroblasts from IL-1β-induced cellular deterioration. Exposure to NaF and BS induced the cell growth and no changes in viability were found with the Lactate Dehydrogenase Assay (LDH) assay at the NaF and BS concentration analyzed. Molecular analysis demonstrated that NaF and BS increased resistance to inflammation by reduction of IL-1β, IL-8, and tumor necrosis factor (TNF)-α production. In addition, NaF and BS decreased the expression of IL-1β, IL-8, and TNF-α mRNA in IL-1β-induced human gingival fibroblast cells. The study identifies a new role for NaF and BS in the IL-1β-induced inflammation of gingival fibroblasts and provides a potential target for gingival protection.http://www.sciencedirect.com/science/article/pii/S1607551X15000935Bamboo saltGingival fibroblast cellsSodium fluoride |
| spellingShingle | Hye-Jin Lee Choong-Ho Choi Anti-inflammatory effects of bamboo salt and sodium fluoride in human gingival fibroblasts–An in vitro study Kaohsiung Journal of Medical Sciences Bamboo salt Gingival fibroblast cells Sodium fluoride |
| title | Anti-inflammatory effects of bamboo salt and sodium fluoride in human gingival fibroblasts–An in vitro study |
| title_full | Anti-inflammatory effects of bamboo salt and sodium fluoride in human gingival fibroblasts–An in vitro study |
| title_fullStr | Anti-inflammatory effects of bamboo salt and sodium fluoride in human gingival fibroblasts–An in vitro study |
| title_full_unstemmed | Anti-inflammatory effects of bamboo salt and sodium fluoride in human gingival fibroblasts–An in vitro study |
| title_short | Anti-inflammatory effects of bamboo salt and sodium fluoride in human gingival fibroblasts–An in vitro study |
| title_sort | anti inflammatory effects of bamboo salt and sodium fluoride in human gingival fibroblasts an in vitro study |
| topic | Bamboo salt Gingival fibroblast cells Sodium fluoride |
| url | http://www.sciencedirect.com/science/article/pii/S1607551X15000935 |
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