Green synthesis of iron (Fe) nanoparticles using Plumeria obtusa extract as a reducing and stabilizing agent: Antimicrobial, antioxidant and biocompatibility studies
In the current study, a green and facile route for the synthesis of iron nanoparticles (FeNPs) was adopted. The FeNPs were fabricated via a single step green route using aqueous leaves extract of Plumeria obtusa (P. obtusa) as a capping/reducing and stabilizing agents. The FeNPs were characterized b...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-05-01
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| Series: | Arabian Journal of Chemistry |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1878535222000806 |
| _version_ | 1811290529218953216 |
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| author | Shazia Perveen Raziya Nadeem Shafiq ur Rehman Nosheen Afzal Shazia Anjum Saima Noreen Rashid Saeed Mongi Amami Samiah H. Al-Mijalli Munawar Iqbal |
| author_facet | Shazia Perveen Raziya Nadeem Shafiq ur Rehman Nosheen Afzal Shazia Anjum Saima Noreen Rashid Saeed Mongi Amami Samiah H. Al-Mijalli Munawar Iqbal |
| author_sort | Shazia Perveen |
| collection | DOAJ |
| description | In the current study, a green and facile route for the synthesis of iron nanoparticles (FeNPs) was adopted. The FeNPs were fabricated via a single step green route using aqueous leaves extract of Plumeria obtusa (P. obtusa) as a capping/reducing and stabilizing agents. The FeNPs were characterized by UV/Vis (Ultraviolet/Visible), FTIR (Fourier Transform Infra-Red spectroscopy), TEM (Transmission Electron Microscopy), SEM (Scanning Electron Microscopy) and XRD (X-Ray Diffraction) techniques. The FeNPs were of spheroidal shape with average size of 50 nm. The biosynthesized FeNPs were further evaluated for their biological activities like antimicrobial, antioxidant and biocompatibility. The FeNPs displayed auspicious antimicrobial activity against bacterial (E. coli, B. subtilis) and fungal strains (A. niger) and S. commune. The test performed against red blood cells (RBCs) lysis (1.22 ± 0.02%) and macrophage (31 ± 0.09%) showed biocompatible nature of FeNPs. In vitro cytotoxicity against AU565 (82.03 ± 0.08–23.65 ± 0.065%) and HeLa (88.61 ± 0.06–33.34 ± 0.06%) cell lines showed cell viability loss in dose dependent manner (FeNPs 25–100 μg/mL). The antioxidant activities values were determined through DPPH, TRPA, NO and H2O2 assays with values 70.23 ± 0.02%, 76.65 ± 0.02 μg AAE/mg, 74.43 ± 0.04% and 67.34 ± 0.03%, respectively. Based on the bioactivities, the green synthesized FeNPs have potential for therapeutic applications. |
| first_indexed | 2024-04-13T04:14:15Z |
| format | Article |
| id | doaj.art-53db74651fe04d9182fad681cd01ccf9 |
| institution | Directory Open Access Journal |
| issn | 1878-5352 |
| language | English |
| last_indexed | 2024-04-13T04:14:15Z |
| publishDate | 2022-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Arabian Journal of Chemistry |
| spelling | doaj.art-53db74651fe04d9182fad681cd01ccf92022-12-22T03:03:01ZengElsevierArabian Journal of Chemistry1878-53522022-05-01155103764Green synthesis of iron (Fe) nanoparticles using Plumeria obtusa extract as a reducing and stabilizing agent: Antimicrobial, antioxidant and biocompatibility studiesShazia Perveen0Raziya Nadeem1Shafiq ur Rehman2Nosheen Afzal3Shazia Anjum4Saima Noreen5Rashid Saeed6Mongi Amami7Samiah H. Al-Mijalli8Munawar Iqbal9Department of Chemistry, University of Agriculture, Faisalabad 38000, PakistanDepartment of Chemistry, University of Agriculture, Faisalabad 38000, Pakistan; Corresponding authors.Department of Chemistry, University of Agriculture, Faisalabad 38000, PakistanDepartment of Biochemistry, Government College University, Faisalabad, PakistanDepartment of Chemistry, The Islamia University of Bahawalpur, 63100 Bahawalpur, PakistanDepartment of Chemistry, University of Agriculture, Faisalabad 38000, PakistanDepartment of Environmental Sciences, University of Gujrat, Gujrat, PakistanDepartment of Chemistry College of Sciences, King Khalid University, P.O. Box 9004, Abha, Saudi Arabia; Laboratoire des matériaux et de l'environnement pour le développement durable LR18ES10, 9 Avenue Dr.Zoheir Safi, 1006 Tunis, TunisiaDepartment of Biology, College of Sciences, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi Arabia; Corresponding authors.Department of Chemistry, Division of Science and Technology, University of Education, Lahore, Pakistan; Corresponding authors.In the current study, a green and facile route for the synthesis of iron nanoparticles (FeNPs) was adopted. The FeNPs were fabricated via a single step green route using aqueous leaves extract of Plumeria obtusa (P. obtusa) as a capping/reducing and stabilizing agents. The FeNPs were characterized by UV/Vis (Ultraviolet/Visible), FTIR (Fourier Transform Infra-Red spectroscopy), TEM (Transmission Electron Microscopy), SEM (Scanning Electron Microscopy) and XRD (X-Ray Diffraction) techniques. The FeNPs were of spheroidal shape with average size of 50 nm. The biosynthesized FeNPs were further evaluated for their biological activities like antimicrobial, antioxidant and biocompatibility. The FeNPs displayed auspicious antimicrobial activity against bacterial (E. coli, B. subtilis) and fungal strains (A. niger) and S. commune. The test performed against red blood cells (RBCs) lysis (1.22 ± 0.02%) and macrophage (31 ± 0.09%) showed biocompatible nature of FeNPs. In vitro cytotoxicity against AU565 (82.03 ± 0.08–23.65 ± 0.065%) and HeLa (88.61 ± 0.06–33.34 ± 0.06%) cell lines showed cell viability loss in dose dependent manner (FeNPs 25–100 μg/mL). The antioxidant activities values were determined through DPPH, TRPA, NO and H2O2 assays with values 70.23 ± 0.02%, 76.65 ± 0.02 μg AAE/mg, 74.43 ± 0.04% and 67.34 ± 0.03%, respectively. Based on the bioactivities, the green synthesized FeNPs have potential for therapeutic applications.http://www.sciencedirect.com/science/article/pii/S1878535222000806BiosynthesisIron nanoparticlesBiocompatibilityAntioxidantCytotoxicityAntimicrobial |
| spellingShingle | Shazia Perveen Raziya Nadeem Shafiq ur Rehman Nosheen Afzal Shazia Anjum Saima Noreen Rashid Saeed Mongi Amami Samiah H. Al-Mijalli Munawar Iqbal Green synthesis of iron (Fe) nanoparticles using Plumeria obtusa extract as a reducing and stabilizing agent: Antimicrobial, antioxidant and biocompatibility studies Arabian Journal of Chemistry Biosynthesis Iron nanoparticles Biocompatibility Antioxidant Cytotoxicity Antimicrobial |
| title | Green synthesis of iron (Fe) nanoparticles using Plumeria obtusa extract as a reducing and stabilizing agent: Antimicrobial, antioxidant and biocompatibility studies |
| title_full | Green synthesis of iron (Fe) nanoparticles using Plumeria obtusa extract as a reducing and stabilizing agent: Antimicrobial, antioxidant and biocompatibility studies |
| title_fullStr | Green synthesis of iron (Fe) nanoparticles using Plumeria obtusa extract as a reducing and stabilizing agent: Antimicrobial, antioxidant and biocompatibility studies |
| title_full_unstemmed | Green synthesis of iron (Fe) nanoparticles using Plumeria obtusa extract as a reducing and stabilizing agent: Antimicrobial, antioxidant and biocompatibility studies |
| title_short | Green synthesis of iron (Fe) nanoparticles using Plumeria obtusa extract as a reducing and stabilizing agent: Antimicrobial, antioxidant and biocompatibility studies |
| title_sort | green synthesis of iron fe nanoparticles using plumeria obtusa extract as a reducing and stabilizing agent antimicrobial antioxidant and biocompatibility studies |
| topic | Biosynthesis Iron nanoparticles Biocompatibility Antioxidant Cytotoxicity Antimicrobial |
| url | http://www.sciencedirect.com/science/article/pii/S1878535222000806 |
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