Metabolomics Analysis Reveals the Effect of Two Alpine Foliar Diseases on the Non-Volatile and Volatile Metabolites of Tea
Blister blight and small leaf spots are important alpine diseases that mainly attack tender tea leaves, affecting tea quality. However, there is limited information on the effect of these diseases on tea’s non-volatile and volatile metabolites. Metabolomic analysis based on UHPLC-Q-TOF/MS, HPLC and...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-04-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/12/8/1568 |
| _version_ | 1797605501920346112 |
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| author | Yuhe Wan Yuxin Han Xinyi Deng Yingjuan Chen |
| author_facet | Yuhe Wan Yuxin Han Xinyi Deng Yingjuan Chen |
| author_sort | Yuhe Wan |
| collection | DOAJ |
| description | Blister blight and small leaf spots are important alpine diseases that mainly attack tender tea leaves, affecting tea quality. However, there is limited information on the effect of these diseases on tea’s non-volatile and volatile metabolites. Metabolomic analysis based on UHPLC-Q-TOF/MS, HPLC and GC/MS was used to reveal the characteristic chemical profiles of tea leaves infected with blister blight (BB) and small leaf spots (SS). Flavonoids and monolignols were non-volatile metabolites that were enriched and significantly changed. Six main monolignols involved in phenylpropanoid biosynthesis were significantly induced in infected tea leaves. The accumulation of catechins, (−)-epigallocatechin gallate, (−)-epicatechin gallate, caffeine, amino acids and theanine were significantly decreased in both diseased tea leaves, while soluble sugar, (−)-epigallocatechin and phenol-ammonia were obviously increased. Among them, the amounts of sweet and umami-related soluble sugar, sucrose, amino acids and theanine were much higher in BB, while bitter and astringent taste-related catechins and derivatives were much higher in SS. Volatiles analysis showed that volatiles content in SS and BB was significantly decreased, and styrene was significantly induced in blister blight-infected tea leaves. The results indicate that the type and amount of volatiles were highly and differentially influenced by infection with the two alpine diseases. |
| first_indexed | 2024-03-11T05:02:00Z |
| format | Article |
| id | doaj.art-53dca8e49b5d4c67be1b047548cb5593 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-11T05:02:00Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-53dca8e49b5d4c67be1b047548cb55932023-11-17T19:13:31ZengMDPI AGFoods2304-81582023-04-01128156810.3390/foods12081568Metabolomics Analysis Reveals the Effect of Two Alpine Foliar Diseases on the Non-Volatile and Volatile Metabolites of TeaYuhe Wan0Yuxin Han1Xinyi Deng2Yingjuan Chen3Department of Tea Science, College of Food Science, Southwest University, Chongqing 400715, ChinaDepartment of Tea Science, College of Food Science, Southwest University, Chongqing 400715, ChinaDepartment of Tea Science, College of Food Science, Southwest University, Chongqing 400715, ChinaDepartment of Tea Science, College of Food Science, Southwest University, Chongqing 400715, ChinaBlister blight and small leaf spots are important alpine diseases that mainly attack tender tea leaves, affecting tea quality. However, there is limited information on the effect of these diseases on tea’s non-volatile and volatile metabolites. Metabolomic analysis based on UHPLC-Q-TOF/MS, HPLC and GC/MS was used to reveal the characteristic chemical profiles of tea leaves infected with blister blight (BB) and small leaf spots (SS). Flavonoids and monolignols were non-volatile metabolites that were enriched and significantly changed. Six main monolignols involved in phenylpropanoid biosynthesis were significantly induced in infected tea leaves. The accumulation of catechins, (−)-epigallocatechin gallate, (−)-epicatechin gallate, caffeine, amino acids and theanine were significantly decreased in both diseased tea leaves, while soluble sugar, (−)-epigallocatechin and phenol-ammonia were obviously increased. Among them, the amounts of sweet and umami-related soluble sugar, sucrose, amino acids and theanine were much higher in BB, while bitter and astringent taste-related catechins and derivatives were much higher in SS. Volatiles analysis showed that volatiles content in SS and BB was significantly decreased, and styrene was significantly induced in blister blight-infected tea leaves. The results indicate that the type and amount of volatiles were highly and differentially influenced by infection with the two alpine diseases.https://www.mdpi.com/2304-8158/12/8/1568tea diseasesmetabolomics analysisnon-volatilesvolatiles |
| spellingShingle | Yuhe Wan Yuxin Han Xinyi Deng Yingjuan Chen Metabolomics Analysis Reveals the Effect of Two Alpine Foliar Diseases on the Non-Volatile and Volatile Metabolites of Tea Foods tea diseases metabolomics analysis non-volatiles volatiles |
| title | Metabolomics Analysis Reveals the Effect of Two Alpine Foliar Diseases on the Non-Volatile and Volatile Metabolites of Tea |
| title_full | Metabolomics Analysis Reveals the Effect of Two Alpine Foliar Diseases on the Non-Volatile and Volatile Metabolites of Tea |
| title_fullStr | Metabolomics Analysis Reveals the Effect of Two Alpine Foliar Diseases on the Non-Volatile and Volatile Metabolites of Tea |
| title_full_unstemmed | Metabolomics Analysis Reveals the Effect of Two Alpine Foliar Diseases on the Non-Volatile and Volatile Metabolites of Tea |
| title_short | Metabolomics Analysis Reveals the Effect of Two Alpine Foliar Diseases on the Non-Volatile and Volatile Metabolites of Tea |
| title_sort | metabolomics analysis reveals the effect of two alpine foliar diseases on the non volatile and volatile metabolites of tea |
| topic | tea diseases metabolomics analysis non-volatiles volatiles |
| url | https://www.mdpi.com/2304-8158/12/8/1568 |
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