MiR-204-5p inhibits proliferation, invasion and migration of thyroid papillary carcinoma K1 cells in vitro by targeting HMGB1 gene
Objective To investigate the effects of miR-204-5p on the proliferation, invasion and migration of thyroid papillary carcinoma K1 cells and its mechanism. Methods K1 cells cultured in vitro were transfected with a miR-204-5p mimics, a miR-204-5p inhibitor, or their respective negative controls (miR-...
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| Format: | Article |
| Language: | zho |
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Editorial Office of Journal of Third Military Medical University
2019-08-01
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| Series: | Di-san junyi daxue xuebao |
| Subjects: | |
| Online Access: | http://aammt.tmmu.edu.cn/Upload/rhtml/201903113.htm |
| _version_ | 1818927037953343488 |
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| author | HAO Junjian HAO Lijuan |
| author_facet | HAO Junjian HAO Lijuan |
| author_sort | HAO Junjian |
| collection | DOAJ |
| description | Objective To investigate the effects of miR-204-5p on the proliferation, invasion and migration of thyroid papillary carcinoma K1 cells and its mechanism. Methods K1 cells cultured in vitro were transfected with a miR-204-5p mimics, a miR-204-5p inhibitor, or their respective negative controls (miR-NC and anti-miR-NC), and the expression of miR-204-5p in the cells was detected using RT-PCR. The changes in cell proliferation were assessed with MTT assay, and the changes in cell invasion and migration were evaluated with Transwell assay. The targeting relationship between miR-204-5p and HMGB1 gene was tested using dual luciferase reporter gene assay, and the regulation of miR-204-5p on the expressions of HMGB1 mRNA and protein was examined using RT-PCR and Western blotting. The changes in the proliferation, invasion and migration of K1 cells were observed after transfection of the cells with siRNA-HMGB1 or a HMGB1 overexpression plasmid. Results The expression level of miR-204-5p was significantly increased in the cells transfected with miR-204-5p mimics and significantly lowered in cells transfected with miR-204-5p inhibitor as compared with their respective controls (P < 0.05). Overexpression of miR-204-5p significantly attenuated while inhibition of miR-204-5p significantly enhanced the proliferation, invasion and migration abilities of the cells in comparison with their respective controls (P < 0.05). HMGB1 was a potential target gene of miR-204-5p, and was negatively regulated by miR-204-5p. RNA interference of HMGB1 obviously inhibited K1 cell proliferation, invasion and migration, while overexpression of HMGB1 promoted the proliferation, invasion and migration of the cells. Conclusion Overexpression of miR-204-5p can inhibit the proliferation, invasion and migration of K1 cells by targeted down-regulation of HMGB1 expression. |
| first_indexed | 2024-12-20T03:06:39Z |
| format | Article |
| id | doaj.art-53dd1feac51547f5b2e718767cefc110 |
| institution | Directory Open Access Journal |
| issn | 1000-5404 |
| language | zho |
| last_indexed | 2024-12-20T03:06:39Z |
| publishDate | 2019-08-01 |
| publisher | Editorial Office of Journal of Third Military Medical University |
| record_format | Article |
| series | Di-san junyi daxue xuebao |
| spelling | doaj.art-53dd1feac51547f5b2e718767cefc1102022-12-21T19:55:35ZzhoEditorial Office of Journal of Third Military Medical UniversityDi-san junyi daxue xuebao1000-54042019-08-0141161559156510.16016/j.1000-5404.201903113MiR-204-5p inhibits proliferation, invasion and migration of thyroid papillary carcinoma K1 cells in vitro by targeting HMGB1 geneHAO Junjian0HAO Lijuan 1Department of Emergency Surgery, Qinghai Red Cross Hospital, Xining, Qinghai Province, 810000, ChinaDepartment of Emergency Surgery, Qinghai Red Cross Hospital, Xining, Qinghai Province, 810000, ChinaObjective To investigate the effects of miR-204-5p on the proliferation, invasion and migration of thyroid papillary carcinoma K1 cells and its mechanism. Methods K1 cells cultured in vitro were transfected with a miR-204-5p mimics, a miR-204-5p inhibitor, or their respective negative controls (miR-NC and anti-miR-NC), and the expression of miR-204-5p in the cells was detected using RT-PCR. The changes in cell proliferation were assessed with MTT assay, and the changes in cell invasion and migration were evaluated with Transwell assay. The targeting relationship between miR-204-5p and HMGB1 gene was tested using dual luciferase reporter gene assay, and the regulation of miR-204-5p on the expressions of HMGB1 mRNA and protein was examined using RT-PCR and Western blotting. The changes in the proliferation, invasion and migration of K1 cells were observed after transfection of the cells with siRNA-HMGB1 or a HMGB1 overexpression plasmid. Results The expression level of miR-204-5p was significantly increased in the cells transfected with miR-204-5p mimics and significantly lowered in cells transfected with miR-204-5p inhibitor as compared with their respective controls (P < 0.05). Overexpression of miR-204-5p significantly attenuated while inhibition of miR-204-5p significantly enhanced the proliferation, invasion and migration abilities of the cells in comparison with their respective controls (P < 0.05). HMGB1 was a potential target gene of miR-204-5p, and was negatively regulated by miR-204-5p. RNA interference of HMGB1 obviously inhibited K1 cell proliferation, invasion and migration, while overexpression of HMGB1 promoted the proliferation, invasion and migration of the cells. Conclusion Overexpression of miR-204-5p can inhibit the proliferation, invasion and migration of K1 cells by targeted down-regulation of HMGB1 expression.http://aammt.tmmu.edu.cn/Upload/rhtml/201903113.htmmir-204-5ppapillary thyroid cancercell proliferationinvasionmigrationhmgb1 |
| spellingShingle | HAO Junjian HAO Lijuan MiR-204-5p inhibits proliferation, invasion and migration of thyroid papillary carcinoma K1 cells in vitro by targeting HMGB1 gene Di-san junyi daxue xuebao mir-204-5p papillary thyroid cancer cell proliferation invasion migration hmgb1 |
| title | MiR-204-5p inhibits proliferation, invasion and migration of thyroid papillary carcinoma K1 cells in vitro by targeting HMGB1 gene |
| title_full | MiR-204-5p inhibits proliferation, invasion and migration of thyroid papillary carcinoma K1 cells in vitro by targeting HMGB1 gene |
| title_fullStr | MiR-204-5p inhibits proliferation, invasion and migration of thyroid papillary carcinoma K1 cells in vitro by targeting HMGB1 gene |
| title_full_unstemmed | MiR-204-5p inhibits proliferation, invasion and migration of thyroid papillary carcinoma K1 cells in vitro by targeting HMGB1 gene |
| title_short | MiR-204-5p inhibits proliferation, invasion and migration of thyroid papillary carcinoma K1 cells in vitro by targeting HMGB1 gene |
| title_sort | mir 204 5p inhibits proliferation invasion and migration of thyroid papillary carcinoma k1 cells in vitro by targeting hmgb1 gene |
| topic | mir-204-5p papillary thyroid cancer cell proliferation invasion migration hmgb1 |
| url | http://aammt.tmmu.edu.cn/Upload/rhtml/201903113.htm |
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