The toxigenic potential of Fusarium poae originated from wheat
Eleven isolates of F. poae, originated from wheat grain at 9 locations mainly in Vojvodina, were encompassed by the present study. The greatest number of samples was collected in 2005, in which the climatic conditions favoured a more intensive occurrence of Fusarium ear blight of wheat. In order to...
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| Format: | Article |
| Language: | English |
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Matica srpska
2007-01-01
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| Series: | Zbornik Matice Srpske za Prirodne Nauke |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0352-4906/2007/0352-49060713113B.pdf |
| _version_ | 1818864875308318720 |
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| author | Bočarov-Stančić Aleksandra S. Lević Jelena T. Stanković Slavica Ž. Krnjaja Vesna S. Kovačević Tamara M. Tančić Sonja L. |
| author_facet | Bočarov-Stančić Aleksandra S. Lević Jelena T. Stanković Slavica Ž. Krnjaja Vesna S. Kovačević Tamara M. Tančić Sonja L. |
| author_sort | Bočarov-Stančić Aleksandra S. |
| collection | DOAJ |
| description | Eleven isolates of F. poae, originated from wheat grain at 9 locations mainly in Vojvodina, were encompassed by the present study. The greatest number of samples was collected in 2005, in which the climatic conditions favoured a more intensive occurrence of Fusarium ear blight of wheat. In order to determine toxicological potential of this species, cultures of the selected isolates were grown in liquid media (GPY and SPY) on a rotary shaker (180 revolutions min-1), at room temperature (21-26°C) for three days. Crude toxins were isolated from liquid culture filtrates of isolates by the use of ethyl acetate, while quantification of mycotoxins was done by the thin layer chromatography method. A liquid culture of the isolate GZ-LES (F. graminearum) was used as a control for the evaluation of the zearalenone biosynthesis potential. On the other hand, the liquid culture of the isolate KF-38/1 (F. sporotrichioides) was used as a control for both type-A trichothecenes (T-2 toxin and diacetoxyscirpenol - DAS). The obtained results show that F. poae, in contrast to F. graminearum, has no potential for the zearalenone biosynthesis. The presence of DAS was determined only in one isolate of F. poae (MRIZP-666), and in the control isolate of F. sporotrichioides (KF-38/1/R), that were grown in the GPY liquid medium. The T-2 toxin was detected in the isolate MRIZP-666, grown in both media, and in the isolates MRIZP-37 and MRIZP-860, cultured in the GPY and SPY liquid medium, respectively. The control culture KF-38/1/R (F. sporotrichioides) produced the T-2 toxin at the concentration of 4,000 μg L-1. According to the gained information, it can be concluded that the potential of F. poae for the type-A trichothecene biosynthesis was low, as the concentration of DAS or T-2 toxin did not exceed 80 μg L-1 or 240 μg L-1, respectively. |
| first_indexed | 2024-12-19T10:38:36Z |
| format | Article |
| id | doaj.art-53e062aee266412f9997f632e250b0e1 |
| institution | Directory Open Access Journal |
| issn | 0352-4906 |
| language | English |
| last_indexed | 2024-12-19T10:38:36Z |
| publishDate | 2007-01-01 |
| publisher | Matica srpska |
| record_format | Article |
| series | Zbornik Matice Srpske za Prirodne Nauke |
| spelling | doaj.art-53e062aee266412f9997f632e250b0e12022-12-21T20:25:32ZengMatica srpskaZbornik Matice Srpske za Prirodne Nauke0352-49062007-01-01200711311312310.2298/ZMSPN0713113BThe toxigenic potential of Fusarium poae originated from wheatBočarov-Stančić Aleksandra S.Lević Jelena T.Stanković Slavica Ž.Krnjaja Vesna S.Kovačević Tamara M.Tančić Sonja L.Eleven isolates of F. poae, originated from wheat grain at 9 locations mainly in Vojvodina, were encompassed by the present study. The greatest number of samples was collected in 2005, in which the climatic conditions favoured a more intensive occurrence of Fusarium ear blight of wheat. In order to determine toxicological potential of this species, cultures of the selected isolates were grown in liquid media (GPY and SPY) on a rotary shaker (180 revolutions min-1), at room temperature (21-26°C) for three days. Crude toxins were isolated from liquid culture filtrates of isolates by the use of ethyl acetate, while quantification of mycotoxins was done by the thin layer chromatography method. A liquid culture of the isolate GZ-LES (F. graminearum) was used as a control for the evaluation of the zearalenone biosynthesis potential. On the other hand, the liquid culture of the isolate KF-38/1 (F. sporotrichioides) was used as a control for both type-A trichothecenes (T-2 toxin and diacetoxyscirpenol - DAS). The obtained results show that F. poae, in contrast to F. graminearum, has no potential for the zearalenone biosynthesis. The presence of DAS was determined only in one isolate of F. poae (MRIZP-666), and in the control isolate of F. sporotrichioides (KF-38/1/R), that were grown in the GPY liquid medium. The T-2 toxin was detected in the isolate MRIZP-666, grown in both media, and in the isolates MRIZP-37 and MRIZP-860, cultured in the GPY and SPY liquid medium, respectively. The control culture KF-38/1/R (F. sporotrichioides) produced the T-2 toxin at the concentration of 4,000 μg L-1. According to the gained information, it can be concluded that the potential of F. poae for the type-A trichothecene biosynthesis was low, as the concentration of DAS or T-2 toxin did not exceed 80 μg L-1 or 240 μg L-1, respectively.http://www.doiserbia.nb.rs/img/doi/0352-4906/2007/0352-49060713113B.pdfdiacetoxyscirpenolF. poaein vitro biosynthesisT-2 toxinwheat |
| spellingShingle | Bočarov-Stančić Aleksandra S. Lević Jelena T. Stanković Slavica Ž. Krnjaja Vesna S. Kovačević Tamara M. Tančić Sonja L. The toxigenic potential of Fusarium poae originated from wheat Zbornik Matice Srpske za Prirodne Nauke diacetoxyscirpenol F. poae in vitro biosynthesis T-2 toxin wheat |
| title | The toxigenic potential of Fusarium poae originated from wheat |
| title_full | The toxigenic potential of Fusarium poae originated from wheat |
| title_fullStr | The toxigenic potential of Fusarium poae originated from wheat |
| title_full_unstemmed | The toxigenic potential of Fusarium poae originated from wheat |
| title_short | The toxigenic potential of Fusarium poae originated from wheat |
| title_sort | toxigenic potential of fusarium poae originated from wheat |
| topic | diacetoxyscirpenol F. poae in vitro biosynthesis T-2 toxin wheat |
| url | http://www.doiserbia.nb.rs/img/doi/0352-4906/2007/0352-49060713113B.pdf |
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