Detection of virulence genes among Staphylococcus saprophyticus isolated from women with urinary tract infections: first report from Iran
Abstract Objective The purpose of the present study was to investigate the biofilm production, and the presence of virulence genes and biochemical characteristics among the S. saprophyticus clinical isolates. A total of 35 clinical isolates of S. saprophyticus were collected from patients referred t...
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| Format: | Article |
| Language: | English |
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BMC
2023-09-01
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| Series: | BMC Research Notes |
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| Online Access: | https://doi.org/10.1186/s13104-023-06481-1 |
| _version_ | 1797578386453823488 |
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| author | Maryam Rafiee Ezzat Allah Ghaemi |
| author_facet | Maryam Rafiee Ezzat Allah Ghaemi |
| author_sort | Maryam Rafiee |
| collection | DOAJ |
| description | Abstract Objective The purpose of the present study was to investigate the biofilm production, and the presence of virulence genes and biochemical characteristics among the S. saprophyticus clinical isolates. A total of 35 clinical isolates of S. saprophyticus were collected from patients referred to several hospitals. By the crystal violet staining method, the capability of biofilm formation was performed. The genes associated with surface of S. saprophyticus were investigated by the PCR-sequencing techniques. Hemagglutination and lipase activity assays were also performed. Results The results of crystal violet staining assay showed that 32 isolates (91%) form biofilm. Moreover, seven (20%), 13 (37%), and 12(34%) isolates were categorized as weak, moderate, and strong biofilm producers, respectively. virulence genes including UafA, Aas and Ssp had an overall prevalence of 88%, 91% and 80%, respectively. None of the isolates exhibited lipolytic activities. Regarding hemagglutination properties, only 11 (31%) isolates demonstrated hemagglutination of sheep erythrocytes. The results of this study indicate a high prevalence of UafA and Aas genes that can enhance the pathogenicity of S. saprophyticus, and Identification and better understanding of the functions of these genes can be used for therapeutic purposes. Maybe in the future we will be switch to anti-adhesion therapy because of drug resistance. |
| first_indexed | 2024-03-10T22:21:13Z |
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| id | doaj.art-53e6f91c013a41059f4afbdf81c06369 |
| institution | Directory Open Access Journal |
| issn | 1756-0500 |
| language | English |
| last_indexed | 2024-03-10T22:21:13Z |
| publishDate | 2023-09-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Research Notes |
| spelling | doaj.art-53e6f91c013a41059f4afbdf81c063692023-11-19T12:15:52ZengBMCBMC Research Notes1756-05002023-09-011611610.1186/s13104-023-06481-1Detection of virulence genes among Staphylococcus saprophyticus isolated from women with urinary tract infections: first report from IranMaryam Rafiee0Ezzat Allah Ghaemi1Laboratory Sciences Research Center, Golestan University of Medical SciencesLaboratory Sciences Research Center, Golestan University of Medical SciencesAbstract Objective The purpose of the present study was to investigate the biofilm production, and the presence of virulence genes and biochemical characteristics among the S. saprophyticus clinical isolates. A total of 35 clinical isolates of S. saprophyticus were collected from patients referred to several hospitals. By the crystal violet staining method, the capability of biofilm formation was performed. The genes associated with surface of S. saprophyticus were investigated by the PCR-sequencing techniques. Hemagglutination and lipase activity assays were also performed. Results The results of crystal violet staining assay showed that 32 isolates (91%) form biofilm. Moreover, seven (20%), 13 (37%), and 12(34%) isolates were categorized as weak, moderate, and strong biofilm producers, respectively. virulence genes including UafA, Aas and Ssp had an overall prevalence of 88%, 91% and 80%, respectively. None of the isolates exhibited lipolytic activities. Regarding hemagglutination properties, only 11 (31%) isolates demonstrated hemagglutination of sheep erythrocytes. The results of this study indicate a high prevalence of UafA and Aas genes that can enhance the pathogenicity of S. saprophyticus, and Identification and better understanding of the functions of these genes can be used for therapeutic purposes. Maybe in the future we will be switch to anti-adhesion therapy because of drug resistance.https://doi.org/10.1186/s13104-023-06481-1Staphylococcus saprophyticusUrinary tract infectionsBiofilmVirulencePolymerase chain reaction |
| spellingShingle | Maryam Rafiee Ezzat Allah Ghaemi Detection of virulence genes among Staphylococcus saprophyticus isolated from women with urinary tract infections: first report from Iran BMC Research Notes Staphylococcus saprophyticus Urinary tract infections Biofilm Virulence Polymerase chain reaction |
| title | Detection of virulence genes among Staphylococcus saprophyticus isolated from women with urinary tract infections: first report from Iran |
| title_full | Detection of virulence genes among Staphylococcus saprophyticus isolated from women with urinary tract infections: first report from Iran |
| title_fullStr | Detection of virulence genes among Staphylococcus saprophyticus isolated from women with urinary tract infections: first report from Iran |
| title_full_unstemmed | Detection of virulence genes among Staphylococcus saprophyticus isolated from women with urinary tract infections: first report from Iran |
| title_short | Detection of virulence genes among Staphylococcus saprophyticus isolated from women with urinary tract infections: first report from Iran |
| title_sort | detection of virulence genes among staphylococcus saprophyticus isolated from women with urinary tract infections first report from iran |
| topic | Staphylococcus saprophyticus Urinary tract infections Biofilm Virulence Polymerase chain reaction |
| url | https://doi.org/10.1186/s13104-023-06481-1 |
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