Calix[4]arene С-956 selectively inhibits plasma membrane Са(2+),Mg(2+)-АТРase in myometrial cells
Using enzymatic assays and kinetic analysis, we demonstrated that 100 µM calix[4]arene C-956 (5,11,17,23-tetra(trifluoro)methyl-(phenylsulfonylimino) methylamino-25,27-dioctyloxy-26,28-dipropoxycalix[4]arene) had the most significant inhibitory effect on the plasma membrane Са2+,Mg2+-АТРase activity...
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| Format: | Article |
| Language: | English |
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National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry
2018-10-01
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| Series: | The Ukrainian Biochemical Journal |
| Subjects: | |
| Online Access: | http://ukrbiochemjournal.org/2018/09/calix4arene-%d1%81-956-selectively-inhibits-plasma-membrane-%d1%81%d0%b02mg2-%d0%b0%d1%82%d1%80ase-in-myometrial-cells.html |
| _version_ | 1797668764588703744 |
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| author | Т. O. Veklich O. A. Skrabak Yu. V. Nikonishyna R. V. Rodik V. I. Kalchenko S. O. Kosterin |
| author_facet | Т. O. Veklich O. A. Skrabak Yu. V. Nikonishyna R. V. Rodik V. I. Kalchenko S. O. Kosterin |
| author_sort | Т. O. Veklich |
| collection | DOAJ |
| description | Using enzymatic assays and kinetic analysis, we demonstrated that 100 µM calix[4]arene C-956 (5,11,17,23-tetra(trifluoro)methyl-(phenylsulfonylimino) methylamino-25,27-dioctyloxy-26,28-dipropoxycalix[4]arene) had the most significant inhibitory effect on the plasma membrane Са2+,Mg2+-АТРase activity compared to effects of other calix[4]arenes, and had no effect on specific activities of other membrane ATPases. Using confocal microscopy and fluorescent probe fluo-4, we observed an increase of the intracellular level of Ca2+ after application of calix[4]arene C-956 to immobilized myocytes. Analysis of the effect of calix[4]arene C-956 on the hydrodynamic diameter of myocytes demonstrated that application of calix[4]arene C-956 solution decreased this parameter by 45.5 ± 9.4% compared to control value similarly to the action of uterotonic drug oxytocin. |
| first_indexed | 2024-03-11T20:34:14Z |
| format | Article |
| id | doaj.art-53eacfd86f514a358a18b299a359ea5a |
| institution | Directory Open Access Journal |
| issn | 2409-4943 2409-4943 |
| language | English |
| last_indexed | 2024-03-11T20:34:14Z |
| publishDate | 2018-10-01 |
| publisher | National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry |
| record_format | Article |
| series | The Ukrainian Biochemical Journal |
| spelling | doaj.art-53eacfd86f514a358a18b299a359ea5a2023-10-02T07:19:19ZengNational Academy of Sciences of Ukraine, Palladin Institute of BiochemistryThe Ukrainian Biochemical Journal2409-49432409-49432018-10-01905344210.15407/ubj90.05.034Calix[4]arene С-956 selectively inhibits plasma membrane Са(2+),Mg(2+)-АТРase in myometrial cellsТ. O. Veklich0 O. A. Skrabak1 Yu. V. Nikonishyna2R. V. Rodik3V. I. Kalchenko4 S. O. Kosterin5Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;Institute of Organic Chemistry, National Academy of Sciences of Ukraine, Kyiv;Institute of Organic Chemistry, National Academy of Sciences of Ukraine, Kyiv;Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;Using enzymatic assays and kinetic analysis, we demonstrated that 100 µM calix[4]arene C-956 (5,11,17,23-tetra(trifluoro)methyl-(phenylsulfonylimino) methylamino-25,27-dioctyloxy-26,28-dipropoxycalix[4]arene) had the most significant inhibitory effect on the plasma membrane Са2+,Mg2+-АТРase activity compared to effects of other calix[4]arenes, and had no effect on specific activities of other membrane ATPases. Using confocal microscopy and fluorescent probe fluo-4, we observed an increase of the intracellular level of Ca2+ after application of calix[4]arene C-956 to immobilized myocytes. Analysis of the effect of calix[4]arene C-956 on the hydrodynamic diameter of myocytes demonstrated that application of calix[4]arene C-956 solution decreased this parameter by 45.5 ± 9.4% compared to control value similarly to the action of uterotonic drug oxytocin.http://ukrbiochemjournal.org/2018/09/calix4arene-%d1%81-956-selectively-inhibits-plasma-membrane-%d1%81%d0%b02mg2-%d0%b0%d1%82%d1%80ase-in-myometrial-cells.htmlCa(2+)-Mg(2+)-ATPasecalix[4]arenesenzymatic hydrolysis of ATPmyometriumplasma membranesmooth muscle cells |
| spellingShingle | Т. O. Veklich O. A. Skrabak Yu. V. Nikonishyna R. V. Rodik V. I. Kalchenko S. O. Kosterin Calix[4]arene С-956 selectively inhibits plasma membrane Са(2+),Mg(2+)-АТРase in myometrial cells The Ukrainian Biochemical Journal Ca(2+)-Mg(2+)-ATPase calix[4]arenes enzymatic hydrolysis of ATP myometrium plasma membrane smooth muscle cells |
| title | Calix[4]arene С-956 selectively inhibits plasma membrane Са(2+),Mg(2+)-АТРase in myometrial cells |
| title_full | Calix[4]arene С-956 selectively inhibits plasma membrane Са(2+),Mg(2+)-АТРase in myometrial cells |
| title_fullStr | Calix[4]arene С-956 selectively inhibits plasma membrane Са(2+),Mg(2+)-АТРase in myometrial cells |
| title_full_unstemmed | Calix[4]arene С-956 selectively inhibits plasma membrane Са(2+),Mg(2+)-АТРase in myometrial cells |
| title_short | Calix[4]arene С-956 selectively inhibits plasma membrane Са(2+),Mg(2+)-АТРase in myometrial cells |
| title_sort | calix 4 arene с 956 selectively inhibits plasma membrane са 2 mg 2 атрase in myometrial cells |
| topic | Ca(2+)-Mg(2+)-ATPase calix[4]arenes enzymatic hydrolysis of ATP myometrium plasma membrane smooth muscle cells |
| url | http://ukrbiochemjournal.org/2018/09/calix4arene-%d1%81-956-selectively-inhibits-plasma-membrane-%d1%81%d0%b02mg2-%d0%b0%d1%82%d1%80ase-in-myometrial-cells.html |
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