To study the relationship between cadmium, zinc and mtDNA copy number in North Indian patients suffering from prostate cancer: A case control study
Objective: To examine the variation of cadmium (Cd), zinc (Zn) and mitochondrial DNA (mtDNA) copy number in prostate cancer (PCa) patients and their age match controls and correlations with clinicopathological parameters. Subjects and methods: This study was conducted between January 2012 and Januar...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2017-06-01
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| Series: | African Journal of Urology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1110570416300856 |
| _version_ | 1819179455266947072 |
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| author | A. Abhishek V. Singh R.J. Sinha N.G. Ansari M.K.J. Siddiqe M. Verma M. Kumar |
| author_facet | A. Abhishek V. Singh R.J. Sinha N.G. Ansari M.K.J. Siddiqe M. Verma M. Kumar |
| author_sort | A. Abhishek |
| collection | DOAJ |
| description | Objective: To examine the variation of cadmium (Cd), zinc (Zn) and mitochondrial DNA (mtDNA) copy number in prostate cancer (PCa) patients and their age match controls and correlations with clinicopathological parameters.
Subjects and methods: This study was conducted between January 2012 and January 2015. Blood Cd and Zn level was measured by inductively coupled plasma spectrophotometer. The variation of mitochondrial copy number in blood was measured by real-time PCR. We scrutinized the correlation of Cd, Zn and mtDNA with clinicopathological parameters like prostate specific antigen (PSA) and [Gleason] score.
Results: Mean Cd level (μg/L ± SD) was significantly higher (3.89 ± 1.49) while Zn level was low (85 ± 10.4) in PCa patients comparison to control patients (2.92 ± 1.23; 116 ± 19.1). A wide distribution of variance and higher mtDNA copy numbers were found in PCa patients [Range (fold difference) 1968–39,245 (17.4)] as compared to controls [10,178–332,243.9], (p value: 0.036). Clinicopathological analysis showed that Cd, Zn and mtDNA DNA copy numbers were significantly associated with increased [Gleason] score but not with serum PSA level (Zn: r = −0.68; Cd: r = 0.64; mtDNA: r = 0.84) (p < 0.05).
Conclusion: Cd, Zn and mtDNA copy number significantly correlate with [Gleason] score which suggests that the former may serve as marker for therapeutic guidance. |
| first_indexed | 2024-12-22T21:58:43Z |
| format | Article |
| id | doaj.art-53eafe0f41e04598a910e07ec5b7014b |
| institution | Directory Open Access Journal |
| issn | 1110-5704 |
| language | English |
| last_indexed | 2024-12-22T21:58:43Z |
| publishDate | 2017-06-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | African Journal of Urology |
| spelling | doaj.art-53eafe0f41e04598a910e07ec5b7014b2022-12-21T18:11:11ZengSpringerOpenAfrican Journal of Urology1110-57042017-06-0123212613210.1016/j.afju.2016.05.011To study the relationship between cadmium, zinc and mtDNA copy number in North Indian patients suffering from prostate cancer: A case control studyA. Abhishek0V. Singh1R.J. Sinha2N.G. Ansari3M.K.J. Siddiqe4M. Verma5M. Kumar6Department of Urology, King George's Medical University, Lucknow 226003, Uttar Pradesh, IndiaDepartment of Urology, King George's Medical University, Lucknow 226003, Uttar Pradesh, IndiaDepartment of Urology, King George's Medical University, Lucknow 226003, Uttar Pradesh, IndiaMetal Analysis Laboratory, CSIR-Indian Institute of Toxicology Research, Lucknow 226001, IndiaCouncil of Science and Technology, Vigyan Bhawan, 9 Nabiullah Road, Lucknow 226018, Uttar Pradesh, IndiaImmunobiology Division, (CSIR)-Indian Institute of Toxicology Research, Post Box No. 80, M.G. Marg, Lucknow 226001, Uttar Pradesh, IndiaAnimal Facility, (CSIR)-Indian Institute of Toxicology Research, Post Box No. 80, M.G. Marg, Lucknow 226001, Uttar Pradesh, IndiaObjective: To examine the variation of cadmium (Cd), zinc (Zn) and mitochondrial DNA (mtDNA) copy number in prostate cancer (PCa) patients and their age match controls and correlations with clinicopathological parameters. Subjects and methods: This study was conducted between January 2012 and January 2015. Blood Cd and Zn level was measured by inductively coupled plasma spectrophotometer. The variation of mitochondrial copy number in blood was measured by real-time PCR. We scrutinized the correlation of Cd, Zn and mtDNA with clinicopathological parameters like prostate specific antigen (PSA) and [Gleason] score. Results: Mean Cd level (μg/L ± SD) was significantly higher (3.89 ± 1.49) while Zn level was low (85 ± 10.4) in PCa patients comparison to control patients (2.92 ± 1.23; 116 ± 19.1). A wide distribution of variance and higher mtDNA copy numbers were found in PCa patients [Range (fold difference) 1968–39,245 (17.4)] as compared to controls [10,178–332,243.9], (p value: 0.036). Clinicopathological analysis showed that Cd, Zn and mtDNA DNA copy numbers were significantly associated with increased [Gleason] score but not with serum PSA level (Zn: r = −0.68; Cd: r = 0.64; mtDNA: r = 0.84) (p < 0.05). Conclusion: Cd, Zn and mtDNA copy number significantly correlate with [Gleason] score which suggests that the former may serve as marker for therapeutic guidance.http://www.sciencedirect.com/science/article/pii/S1110570416300856Mitochondrial DNA copy numberCadmiumZincProstate cancerRT-PCRGleason score |
| spellingShingle | A. Abhishek V. Singh R.J. Sinha N.G. Ansari M.K.J. Siddiqe M. Verma M. Kumar To study the relationship between cadmium, zinc and mtDNA copy number in North Indian patients suffering from prostate cancer: A case control study African Journal of Urology Mitochondrial DNA copy number Cadmium Zinc Prostate cancer RT-PCR Gleason score |
| title | To study the relationship between cadmium, zinc and mtDNA copy number in North Indian patients suffering from prostate cancer: A case control study |
| title_full | To study the relationship between cadmium, zinc and mtDNA copy number in North Indian patients suffering from prostate cancer: A case control study |
| title_fullStr | To study the relationship between cadmium, zinc and mtDNA copy number in North Indian patients suffering from prostate cancer: A case control study |
| title_full_unstemmed | To study the relationship between cadmium, zinc and mtDNA copy number in North Indian patients suffering from prostate cancer: A case control study |
| title_short | To study the relationship between cadmium, zinc and mtDNA copy number in North Indian patients suffering from prostate cancer: A case control study |
| title_sort | to study the relationship between cadmium zinc and mtdna copy number in north indian patients suffering from prostate cancer a case control study |
| topic | Mitochondrial DNA copy number Cadmium Zinc Prostate cancer RT-PCR Gleason score |
| url | http://www.sciencedirect.com/science/article/pii/S1110570416300856 |
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