Resistance to Tomato yellow leaf curl virus Accumulation in the Tomato Wild Relative Solanum habrochaites Associated with the C4 Viral Protein
Tomato yellow leaf curl disease (TYLCD) is a severe threat to tomato crops worldwide and is caused by Tomato yellow leaf curl virus (TYLCV) and several other begomoviruses (genus Begomovirus, family Geminiviridae). Host plant resistance is the best TYLCD control method but limited sources of resista...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2011-07-01
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| Series: | Molecular Plant-Microbe Interactions |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI-12-10-0291 |
| _version_ | 1811287293271474176 |
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| author | Diego M. Tomás M. Carmen Cañizares Jesús Abad Rafael Fernández-Muñoz Enrique Moriones |
| author_facet | Diego M. Tomás M. Carmen Cañizares Jesús Abad Rafael Fernández-Muñoz Enrique Moriones |
| author_sort | Diego M. Tomás |
| collection | DOAJ |
| description | Tomato yellow leaf curl disease (TYLCD) is a severe threat to tomato crops worldwide and is caused by Tomato yellow leaf curl virus (TYLCV) and several other begomoviruses (genus Begomovirus, family Geminiviridae). Host plant resistance is the best TYLCD control method but limited sources of resistance are available. In this study, two Solanum habrochaites TYLCD-resistance sources, EELM-388 and EELM-889, were found after a wide germplasm screening and were further characterized. A consistent resistance to the widely distributed strain TYLCV-IL was observed when plants were inoculated by Bemisia tabaci or by agroinoculation using an infectious clone, with no symptoms or virus accumulation observed in inoculated plants. Moreover, the resistance was effective under field conditions with high TYLCD pressure. Two independent loci, one dominant and one recessive, were associated with EELM-889 resistance. The study shows these loci to be distinct from that of the resistance gene (Ty-1 gene) commonly deployed in commercial tomato cultivars. Therefore, both kinds of resistance could be combined to provide improved resistance to TYLCD. Four additional TYLCD-associated viruses were challenged, showing that the resistance always prevented symptom expression, although systemic infection could occur in some cases. By using chimeric and mutant expression constructs, the C4 protein was shown to be associated with the ability to result in effective systemic infection. |
| first_indexed | 2024-04-13T03:16:23Z |
| format | Article |
| id | doaj.art-53eb32b48b5a44539ceb564b6d7402cb |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-04-13T03:16:23Z |
| publishDate | 2011-07-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-53eb32b48b5a44539ceb564b6d7402cb2022-12-22T03:04:54ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062011-07-0124784986110.1094/MPMI-12-10-0291Resistance to Tomato yellow leaf curl virus Accumulation in the Tomato Wild Relative Solanum habrochaites Associated with the C4 Viral ProteinDiego M. TomásM. Carmen CañizaresJesús AbadRafael Fernández-MuñozEnrique MorionesTomato yellow leaf curl disease (TYLCD) is a severe threat to tomato crops worldwide and is caused by Tomato yellow leaf curl virus (TYLCV) and several other begomoviruses (genus Begomovirus, family Geminiviridae). Host plant resistance is the best TYLCD control method but limited sources of resistance are available. In this study, two Solanum habrochaites TYLCD-resistance sources, EELM-388 and EELM-889, were found after a wide germplasm screening and were further characterized. A consistent resistance to the widely distributed strain TYLCV-IL was observed when plants were inoculated by Bemisia tabaci or by agroinoculation using an infectious clone, with no symptoms or virus accumulation observed in inoculated plants. Moreover, the resistance was effective under field conditions with high TYLCD pressure. Two independent loci, one dominant and one recessive, were associated with EELM-889 resistance. The study shows these loci to be distinct from that of the resistance gene (Ty-1 gene) commonly deployed in commercial tomato cultivars. Therefore, both kinds of resistance could be combined to provide improved resistance to TYLCD. Four additional TYLCD-associated viruses were challenged, showing that the resistance always prevented symptom expression, although systemic infection could occur in some cases. By using chimeric and mutant expression constructs, the C4 protein was shown to be associated with the ability to result in effective systemic infection.https://apsjournals.apsnet.org/doi/10.1094/MPMI-12-10-0291 |
| spellingShingle | Diego M. Tomás M. Carmen Cañizares Jesús Abad Rafael Fernández-Muñoz Enrique Moriones Resistance to Tomato yellow leaf curl virus Accumulation in the Tomato Wild Relative Solanum habrochaites Associated with the C4 Viral Protein Molecular Plant-Microbe Interactions |
| title | Resistance to Tomato yellow leaf curl virus Accumulation in the Tomato Wild Relative Solanum habrochaites Associated with the C4 Viral Protein |
| title_full | Resistance to Tomato yellow leaf curl virus Accumulation in the Tomato Wild Relative Solanum habrochaites Associated with the C4 Viral Protein |
| title_fullStr | Resistance to Tomato yellow leaf curl virus Accumulation in the Tomato Wild Relative Solanum habrochaites Associated with the C4 Viral Protein |
| title_full_unstemmed | Resistance to Tomato yellow leaf curl virus Accumulation in the Tomato Wild Relative Solanum habrochaites Associated with the C4 Viral Protein |
| title_short | Resistance to Tomato yellow leaf curl virus Accumulation in the Tomato Wild Relative Solanum habrochaites Associated with the C4 Viral Protein |
| title_sort | resistance to tomato yellow leaf curl virus accumulation in the tomato wild relative solanum habrochaites associated with the c4 viral protein |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI-12-10-0291 |
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