Screening of Single-Stranded DNA Aptamer Specific for Florfenicol and Application in Detection of Food Safety

Screening of Single-Stranded DNA Aptamer Specific for Florfenicol and Application in Detection of Food Safety

In this work, the single-stranded DNA (ssDNA) aptamers specific to florfenicol (FF) and having a high binding affinity were prepared using the magnetic bead-based systematic evolution of ligands by the exponential enrichment technique (MB-SELEX). After 10 rounds of the MB-SELEX screening, aptamers t...

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Bibliographic Details
Main Authors: Minghui Shi, Ruobing Liu, Fuyuan Zhang, Bimal Chitrakar, Xianghong Wang
Format: Article
Language:English
Published: MDPI AG 2022-09-01
Series:Biosensors
Subjects:
florfenicol
SELEX
aptamer
AuNPs
molecular docking
Online Access:https://www.mdpi.com/2079-6374/12/9/701

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