Insoluble chitosan complex as a potential adsorbent for aflatoxin B1 in poultry feed
As a class of secondary metabolites or toxins produced by fungi, aflatoxins can poison humans and animals; among them, aflatoxin B1 (AFB1) is the most dangerous one owing to its carcinogenic and mutagenic properties that increase risks for hepatocellular carcinoma in humans; hence, adsorbents such a...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-12-01
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| Series: | Frontiers in Materials |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmats.2022.1044495/full |
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| author | Armando Delgado-Cedeño Sara Paola Hernández-Martínez Sara Paola Hernández-Martínez Yareellys Ramos-Zayas Yareellys Ramos-Zayas Alicia Guadalupe Marroquín-Cardona Gerardo Méndez-Zamora Gerardo Méndez-Zamora Moisés Armides Franco-Molina Jorge R. Kawas Jorge R. Kawas |
| author_facet | Armando Delgado-Cedeño Sara Paola Hernández-Martínez Sara Paola Hernández-Martínez Yareellys Ramos-Zayas Yareellys Ramos-Zayas Alicia Guadalupe Marroquín-Cardona Gerardo Méndez-Zamora Gerardo Méndez-Zamora Moisés Armides Franco-Molina Jorge R. Kawas Jorge R. Kawas |
| author_sort | Armando Delgado-Cedeño |
| collection | DOAJ |
| description | As a class of secondary metabolites or toxins produced by fungi, aflatoxins can poison humans and animals; among them, aflatoxin B1 (AFB1) is the most dangerous one owing to its carcinogenic and mutagenic properties that increase risks for hepatocellular carcinoma in humans; hence, adsorbents such as smectites are commonly included in poultry feed to mitigate their effects. In this study, chitosan was crosslinked with sodium dodecyl sulfate (SDS) to form an insoluble polymer complex that is stable at the relevant physiological pH levels. The characterization via Fourier transforms infrared spectroscopy revealed the interaction between the sulfate groups of the SDS and the amine group of chitosan (1,016 and 819 cm−1); this result was further confirmed by the X-ray diffraction patterns with a change in the crystalline structure of the chitosan-insoluble complex (2θ = 4.76°, 7°, and 22°). The morphology of the chitosan-insoluble complex obtained using a field emission scanning electron microscope (FE-SEM) revealed that particles were slightly porous. After characterization, the performance of the chemically modified polymer complex was evaluated as an adsorbent for AFB1 and compared with those of the unmodified chitosan, soluble chitosan complex, and commercial montmorillonite clay binder. In addition, the polymer complex was investigated as an adsorbent in an in vitro model for the poultry gastrointestinal system. Sequestration of AFB1 by a chemically modified polymer complex was 93.4%, equivalent to that of commercial montmorillonite clay (99.5%). However, these treatments also sequestered microminerals, particularly selenium and iron. This pH-stable, high-capacity adsorbent could be used in poultry feed to reduce the uptake of AFB1. |
| first_indexed | 2024-04-11T13:44:01Z |
| format | Article |
| id | doaj.art-53ef0825a5424d6bb44289975445a50b |
| institution | Directory Open Access Journal |
| issn | 2296-8016 |
| language | English |
| last_indexed | 2024-04-11T13:44:01Z |
| publishDate | 2022-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Materials |
| spelling | doaj.art-53ef0825a5424d6bb44289975445a50b2022-12-22T04:21:09ZengFrontiers Media S.A.Frontiers in Materials2296-80162022-12-01910.3389/fmats.2022.10444951044495Insoluble chitosan complex as a potential adsorbent for aflatoxin B1 in poultry feedArmando Delgado-Cedeño0Sara Paola Hernández-Martínez1Sara Paola Hernández-Martínez2Yareellys Ramos-Zayas3Yareellys Ramos-Zayas4Alicia Guadalupe Marroquín-Cardona5Gerardo Méndez-Zamora6Gerardo Méndez-Zamora7Moisés Armides Franco-Molina8Jorge R. Kawas9Jorge R. Kawas10Universidad Autónoma de Nuevo León, Posgrado Conjunto Agronomía-Veterinaria, Nuevo León, MéxicoUniversidad Autónoma de Nuevo León, Facultad de Agronomía, Nuevo León, MéxicoMNA de México, Nuevo León, MéxicoUniversidad Autónoma de Nuevo León, Posgrado Conjunto Agronomía-Veterinaria, Nuevo León, MéxicoMNA de México, Nuevo León, MéxicoUniversidad Autónoma de Nuevo León, Posgrado Conjunto Agronomía-Veterinaria, Nuevo León, MéxicoUniversidad Autónoma de Nuevo León, Posgrado Conjunto Agronomía-Veterinaria, Nuevo León, MéxicoUniversidad Autónoma de Nuevo León, Facultad de Agronomía, Nuevo León, MéxicoUniversidad Autónoma de Nuevo León, Facultad de Biología, Nuevo León, MéxicoUniversidad Autónoma de Nuevo León, Posgrado Conjunto Agronomía-Veterinaria, Nuevo León, MéxicoMNA de México, Nuevo León, MéxicoAs a class of secondary metabolites or toxins produced by fungi, aflatoxins can poison humans and animals; among them, aflatoxin B1 (AFB1) is the most dangerous one owing to its carcinogenic and mutagenic properties that increase risks for hepatocellular carcinoma in humans; hence, adsorbents such as smectites are commonly included in poultry feed to mitigate their effects. In this study, chitosan was crosslinked with sodium dodecyl sulfate (SDS) to form an insoluble polymer complex that is stable at the relevant physiological pH levels. The characterization via Fourier transforms infrared spectroscopy revealed the interaction between the sulfate groups of the SDS and the amine group of chitosan (1,016 and 819 cm−1); this result was further confirmed by the X-ray diffraction patterns with a change in the crystalline structure of the chitosan-insoluble complex (2θ = 4.76°, 7°, and 22°). The morphology of the chitosan-insoluble complex obtained using a field emission scanning electron microscope (FE-SEM) revealed that particles were slightly porous. After characterization, the performance of the chemically modified polymer complex was evaluated as an adsorbent for AFB1 and compared with those of the unmodified chitosan, soluble chitosan complex, and commercial montmorillonite clay binder. In addition, the polymer complex was investigated as an adsorbent in an in vitro model for the poultry gastrointestinal system. Sequestration of AFB1 by a chemically modified polymer complex was 93.4%, equivalent to that of commercial montmorillonite clay (99.5%). However, these treatments also sequestered microminerals, particularly selenium and iron. This pH-stable, high-capacity adsorbent could be used in poultry feed to reduce the uptake of AFB1.https://www.frontiersin.org/articles/10.3389/fmats.2022.1044495/fullchitosanpolymerscrosslinkingsodium dodecyl sulfateaflatoxin B1in vitro poultry gastrointestinal model |
| spellingShingle | Armando Delgado-Cedeño Sara Paola Hernández-Martínez Sara Paola Hernández-Martínez Yareellys Ramos-Zayas Yareellys Ramos-Zayas Alicia Guadalupe Marroquín-Cardona Gerardo Méndez-Zamora Gerardo Méndez-Zamora Moisés Armides Franco-Molina Jorge R. Kawas Jorge R. Kawas Insoluble chitosan complex as a potential adsorbent for aflatoxin B1 in poultry feed Frontiers in Materials chitosan polymers crosslinking sodium dodecyl sulfate aflatoxin B1 in vitro poultry gastrointestinal model |
| title | Insoluble chitosan complex as a potential adsorbent for aflatoxin B1 in poultry feed |
| title_full | Insoluble chitosan complex as a potential adsorbent for aflatoxin B1 in poultry feed |
| title_fullStr | Insoluble chitosan complex as a potential adsorbent for aflatoxin B1 in poultry feed |
| title_full_unstemmed | Insoluble chitosan complex as a potential adsorbent for aflatoxin B1 in poultry feed |
| title_short | Insoluble chitosan complex as a potential adsorbent for aflatoxin B1 in poultry feed |
| title_sort | insoluble chitosan complex as a potential adsorbent for aflatoxin b1 in poultry feed |
| topic | chitosan polymers crosslinking sodium dodecyl sulfate aflatoxin B1 in vitro poultry gastrointestinal model |
| url | https://www.frontiersin.org/articles/10.3389/fmats.2022.1044495/full |
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