Visual detection of porcine epidemic diarrhea virus by recombinase polymerase amplification combined with lateral flow dipstrip
Abstract Background Porcine epidemic diarrhea virus (PEDV) is one of the most important enteric viruses causing diarrhea in pigs. The establishment of a rapid detection method applicable in field conditions will be conducive to early detection of pathogen and implementation of relevant treatment. A...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-04-01
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| Series: | BMC Veterinary Research |
| Online Access: | https://doi.org/10.1186/s12917-022-03232-5 |
| _version_ | 1818147413471264768 |
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| author | Lei Ma Kaiqi Lian Mengjie Zhu Yajie Tang Mingliang Zhang |
| author_facet | Lei Ma Kaiqi Lian Mengjie Zhu Yajie Tang Mingliang Zhang |
| author_sort | Lei Ma |
| collection | DOAJ |
| description | Abstract Background Porcine epidemic diarrhea virus (PEDV) is one of the most important enteric viruses causing diarrhea in pigs. The establishment of a rapid detection method applicable in field conditions will be conducive to early detection of pathogen and implementation of relevant treatment. A novel nucleic acid amplification method, recombinase polymerase amplification (RPA), has been widely used for infectious disease diagnosis. Results In the present study, a reverse transcription (RT)-RPA assay combined with lateral flow dipstrip (LFD) was established for the visual detection of PEDV by targeting the N gene. The RT-RPA-LFD assay detected as low as 102 copies/µL of PEDV genomic RNA standard. Moreover, the novel RT-RPA-LFD assay did not show cross-reactivity with common swine pathogens, demonstrating high specificity. The performance of the assay for detection of clinical samples was also evaluated. A total number of 86 clinical samples were tested by RT-RPA-LFD and RT-PCR. The detection results of RT-RPA-LFD were compared with those of RT-PCR, with a coincidence rate of 96.5%. Conclusion The newly established RT-RPA-LFD assay in our study had high sensitivity and specificity, with a potential to use in resource-limited areas and countries. |
| first_indexed | 2024-12-11T12:34:51Z |
| format | Article |
| id | doaj.art-53ef148fbca24d7699cd605ef88cf4bc |
| institution | Directory Open Access Journal |
| issn | 1746-6148 |
| language | English |
| last_indexed | 2024-12-11T12:34:51Z |
| publishDate | 2022-04-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Veterinary Research |
| spelling | doaj.art-53ef148fbca24d7699cd605ef88cf4bc2022-12-22T01:07:10ZengBMCBMC Veterinary Research1746-61482022-04-011811810.1186/s12917-022-03232-5Visual detection of porcine epidemic diarrhea virus by recombinase polymerase amplification combined with lateral flow dipstripLei Ma0Kaiqi Lian1Mengjie Zhu2Yajie Tang3Mingliang Zhang4School of Biotechnology and Food Engineering, Anyang Institute of TechnologySchool of Biotechnology and Food Engineering, Anyang Institute of TechnologySchool of Biotechnology and Food Engineering, Anyang Institute of TechnologySchool of Biotechnology and Food Engineering, Anyang Institute of TechnologySchool of Biotechnology and Food Engineering, Anyang Institute of TechnologyAbstract Background Porcine epidemic diarrhea virus (PEDV) is one of the most important enteric viruses causing diarrhea in pigs. The establishment of a rapid detection method applicable in field conditions will be conducive to early detection of pathogen and implementation of relevant treatment. A novel nucleic acid amplification method, recombinase polymerase amplification (RPA), has been widely used for infectious disease diagnosis. Results In the present study, a reverse transcription (RT)-RPA assay combined with lateral flow dipstrip (LFD) was established for the visual detection of PEDV by targeting the N gene. The RT-RPA-LFD assay detected as low as 102 copies/µL of PEDV genomic RNA standard. Moreover, the novel RT-RPA-LFD assay did not show cross-reactivity with common swine pathogens, demonstrating high specificity. The performance of the assay for detection of clinical samples was also evaluated. A total number of 86 clinical samples were tested by RT-RPA-LFD and RT-PCR. The detection results of RT-RPA-LFD were compared with those of RT-PCR, with a coincidence rate of 96.5%. Conclusion The newly established RT-RPA-LFD assay in our study had high sensitivity and specificity, with a potential to use in resource-limited areas and countries.https://doi.org/10.1186/s12917-022-03232-5 |
| spellingShingle | Lei Ma Kaiqi Lian Mengjie Zhu Yajie Tang Mingliang Zhang Visual detection of porcine epidemic diarrhea virus by recombinase polymerase amplification combined with lateral flow dipstrip BMC Veterinary Research |
| title | Visual detection of porcine epidemic diarrhea virus by recombinase polymerase amplification combined with lateral flow dipstrip |
| title_full | Visual detection of porcine epidemic diarrhea virus by recombinase polymerase amplification combined with lateral flow dipstrip |
| title_fullStr | Visual detection of porcine epidemic diarrhea virus by recombinase polymerase amplification combined with lateral flow dipstrip |
| title_full_unstemmed | Visual detection of porcine epidemic diarrhea virus by recombinase polymerase amplification combined with lateral flow dipstrip |
| title_short | Visual detection of porcine epidemic diarrhea virus by recombinase polymerase amplification combined with lateral flow dipstrip |
| title_sort | visual detection of porcine epidemic diarrhea virus by recombinase polymerase amplification combined with lateral flow dipstrip |
| url | https://doi.org/10.1186/s12917-022-03232-5 |
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