Antibiotic Resistance and RAPD-PCR Genotyping of Pseudomonas Aeruginosa Clinical Strains Isolated from Intensive Care Unit Patients
Background: Pseudomonas aeruginosa is one the most important nosocomial pathogens, especially in immunocompromised patients. Identifying the source of contamination in health centers plays an important role in the control of hospital infections. The aim of this study was to determine antibiotic susc...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Shahid Beheshti University of Medical Sciences
2021-06-01
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| Series: | Novelty in Biomedicine |
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| Online Access: | https://journals.sbmu.ac.ir/nbm/article/view/32774 |
| _version_ | 1818647901556965376 |
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| author | Mona Ghazi Hossein Goudarzi Masoud Dadashi Donya Taghizadeh Maleki Parisa Abedi Ilkhichi Mehdi Goudarzi Aghil Bahramian Abbas Yadegar |
| author_facet | Mona Ghazi Hossein Goudarzi Masoud Dadashi Donya Taghizadeh Maleki Parisa Abedi Ilkhichi Mehdi Goudarzi Aghil Bahramian Abbas Yadegar |
| author_sort | Mona Ghazi |
| collection | DOAJ |
| description | Background: Pseudomonas aeruginosa is one the most important nosocomial pathogens, especially in immunocompromised patients. Identifying the source of contamination in health centers plays an important role in the control of hospital infections. The aim of this study was to determine antibiotic susceptibility and genetic patterns of P. aeruginosa isolated from patients hospitalized in intensive care unit of Masih Daneshvari Hospital, Tehran, Iran.
Materials and Methods: Antibiotic susceptibility of the isolates was examined through 10 antibiotics recommended by Clinical and Laboratory Standards Institute (CLSI, 2018) guidelines using the Kirby-Bauer disc diffusion method. Random amplified polymorphic DNA (RAPD) analysis with the short primer of 272 was used to evaluate genetic relationship among the isolates and the results were analyzed by Gelcompar II software.
Results: Of the antibiotics used, the most sensitive was found in colistin (96.4%) and the highest resistance rates were observed in cefotaxime (94.6%), chloramphenicol (83.9%) and imipenem (71.4%). DNA fingerprinting was able to identify 12 genetic patterns by RAPD-PCR technique.
Conclusion: Antibiotic resistance in isolates of P. aeruginosa is rising and there is possibility of occurring outbreaks in the medical centers. Different sources of strains show their constant exchange via intra- and extra-hospital transmission routes. Thus, according to the data of this study, there is a serious need to control sources of infections by physicians and staff when they are working in several sectors to control and prevent the transmission of the bacterium. |
| first_indexed | 2024-12-17T01:09:54Z |
| format | Article |
| id | doaj.art-53f1ca1800084b6e99567c785246fb5e |
| institution | Directory Open Access Journal |
| issn | 2345-3907 |
| language | English |
| last_indexed | 2024-12-17T01:09:54Z |
| publishDate | 2021-06-01 |
| publisher | Shahid Beheshti University of Medical Sciences |
| record_format | Article |
| series | Novelty in Biomedicine |
| spelling | doaj.art-53f1ca1800084b6e99567c785246fb5e2022-12-21T22:09:10ZengShahid Beheshti University of Medical SciencesNovelty in Biomedicine2345-39072021-06-0192525710.22037/nbm.v9i2.3277432774Antibiotic Resistance and RAPD-PCR Genotyping of Pseudomonas Aeruginosa Clinical Strains Isolated from Intensive Care Unit PatientsMona GhaziHossein GoudarziMasoud DadashiDonya Taghizadeh MalekiParisa Abedi IlkhichiMehdi GoudarziAghil BahramianAbbas YadegarBackground: Pseudomonas aeruginosa is one the most important nosocomial pathogens, especially in immunocompromised patients. Identifying the source of contamination in health centers plays an important role in the control of hospital infections. The aim of this study was to determine antibiotic susceptibility and genetic patterns of P. aeruginosa isolated from patients hospitalized in intensive care unit of Masih Daneshvari Hospital, Tehran, Iran. Materials and Methods: Antibiotic susceptibility of the isolates was examined through 10 antibiotics recommended by Clinical and Laboratory Standards Institute (CLSI, 2018) guidelines using the Kirby-Bauer disc diffusion method. Random amplified polymorphic DNA (RAPD) analysis with the short primer of 272 was used to evaluate genetic relationship among the isolates and the results were analyzed by Gelcompar II software. Results: Of the antibiotics used, the most sensitive was found in colistin (96.4%) and the highest resistance rates were observed in cefotaxime (94.6%), chloramphenicol (83.9%) and imipenem (71.4%). DNA fingerprinting was able to identify 12 genetic patterns by RAPD-PCR technique. Conclusion: Antibiotic resistance in isolates of P. aeruginosa is rising and there is possibility of occurring outbreaks in the medical centers. Different sources of strains show their constant exchange via intra- and extra-hospital transmission routes. Thus, according to the data of this study, there is a serious need to control sources of infections by physicians and staff when they are working in several sectors to control and prevent the transmission of the bacterium.https://journals.sbmu.ac.ir/nbm/article/view/32774pseudomonas aeruginosa, multi-drug resistance, rapd-pc, icu |
| spellingShingle | Mona Ghazi Hossein Goudarzi Masoud Dadashi Donya Taghizadeh Maleki Parisa Abedi Ilkhichi Mehdi Goudarzi Aghil Bahramian Abbas Yadegar Antibiotic Resistance and RAPD-PCR Genotyping of Pseudomonas Aeruginosa Clinical Strains Isolated from Intensive Care Unit Patients Novelty in Biomedicine pseudomonas aeruginosa, multi-drug resistance, rapd-pc, icu |
| title | Antibiotic Resistance and RAPD-PCR Genotyping of Pseudomonas Aeruginosa Clinical Strains Isolated from Intensive Care Unit Patients |
| title_full | Antibiotic Resistance and RAPD-PCR Genotyping of Pseudomonas Aeruginosa Clinical Strains Isolated from Intensive Care Unit Patients |
| title_fullStr | Antibiotic Resistance and RAPD-PCR Genotyping of Pseudomonas Aeruginosa Clinical Strains Isolated from Intensive Care Unit Patients |
| title_full_unstemmed | Antibiotic Resistance and RAPD-PCR Genotyping of Pseudomonas Aeruginosa Clinical Strains Isolated from Intensive Care Unit Patients |
| title_short | Antibiotic Resistance and RAPD-PCR Genotyping of Pseudomonas Aeruginosa Clinical Strains Isolated from Intensive Care Unit Patients |
| title_sort | antibiotic resistance and rapd pcr genotyping of pseudomonas aeruginosa clinical strains isolated from intensive care unit patients |
| topic | pseudomonas aeruginosa, multi-drug resistance, rapd-pc, icu |
| url | https://journals.sbmu.ac.ir/nbm/article/view/32774 |
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