Comparison of methylation estimates obtained via MinION nanopore sequencing and sanger bisulfite sequencing in the TRPA1 promoter region

Comparison of methylation estimates obtained via MinION nanopore sequencing and sanger bisulfite sequencing in the TRPA1 promoter region

Abstract Background Bisulfite sequencing has long been considered the gold standard for measuring DNA methylation at single CpG resolution. However, in recent years several new approaches like nanopore sequencing have been developed due to hints for a partial error-proneness of bisulfite sequencing....

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Bibliographic Details
Main Authors: Sara Gombert, Kirsten Jahn, Hansi Pathak, Alexandra Burkert, Gunnar Schmidt, Lutz Wiehlmann, Colin Davenport, Björn Brändl, Franz-Josef Müller, Andreas Leffler, Maximilian Deest, Helge Frieling
Format: Article
Language:English
Published: BMC 2023-10-01
Series:BMC Medical Genomics
Subjects:
TRPA1
Epigenetics
Methylation
Bisulfite sequencing
Nanopore sequencing
Cas9-mediated PCR-free enrichment, guideRNAs
Online Access:https://doi.org/10.1186/s12920-023-01694-6

Internet

https://doi.org/10.1186/s12920-023-01694-6

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